Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

The obligate intracellular bacterium Chlamydia psittaci is a recognized avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to important mortality if inadequately recognized and handled. Though intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been troublesome to show host-pathogen interactions in C. […]

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Plasmid-mediated gene remedy, being a protected and comparatively cheap therapeutic technique, is stricken by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. We have now earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the […]

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to several types of stress. Genome enhancing of P. putida largely depends on homologous recombination occasions, assisted by helper plasmid-based expression of genes encoding […]

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those plasmids from their bacterial cells is a vital step in figuring out strain-specific virulence traits and to assemble strains helpful […]

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Methylflash Methylated DNA 5-mc Quantification Kit (colorimetric)

Product description The MethylFlash ™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of buffers and reagents optimized to colourimetrically quantify the global methylation status of DNA by specifically measuring 5-methylcytosine (5-mC) levels in a simplified format. , “one-step” reaction similar to ELISA. This allows global DNA methylation to be directly measured […]

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Human Anti KLH IgG

Product name Keyhole Limpet Hemocyanin (KLH) Antibody (IgG), ELISA Kit Full Product Name Keyhole Limpet Hemocyanin Antibody (KLH), IgG ELISA Kit Product synonym names Keyhole Limpet Hemocyanin (KLH) Antibody (IgG) ELISA Kit; Keyhole Limpet Hemocyanin (KLH) Antibody (IgG) Species reactivity: Human Specificity This assay has high sensitivity and excellent specificity for the detection of human […]

Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

Sirius Red/Fast Green Collagen Staining Kit

Product description Item Name: Fast Sirius Red / Green Collagen Staining Kit Biozol catalogue number: CHX-9046 Supplier Catalog Number: 9046 Alternate Catalog Number: CHX-9046 Manufacturer: Chondrex Category: Kits / Assays Product properties Range: Collagen: 3 µg / section Other proteins: 50 µg / section Storage: RT Introduction Sirius Red specifically binds to the [Gly-X-Y] n […]

Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917

HemogloBind

Removal and Capture of Hemoglobin HemogloBind ™ Has a high degree of specificity for haemoglobin binding up to 10 mg / ml Applications in blood substitutes, enzyme recovery and analytical interference Eliminates haemoglobin of any species, including humans, sheep, cattle, goats, etc. Eliminates haemoglobin from organs, tissues. Removal of haemoglobin from red blood cell lysate […]