Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

Development of a Plasmid Shuttle Vector System for…

The obligate intracellular bacterium Chlamydia psittaci is a recognized avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to important mortality if inadequately recognized and handled.…
[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

[Maintenance of Plasmid Expression in vivo Depends Primarily…

Plasmid-mediated gene remedy, being a protected and comparatively cheap therapeutic technique, is stricken by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. We have now earlier constructed…
Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Synthetic control of plasmid replication enables target- and…

Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to…
Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Effective removal of a range of Ti/Ri plasmids…

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those…