Recurrence after liver resection for hepatocellular carcinoma (HCC) is a serious medical drawback, and prognostic markers for recurrence are urgently required. For 390 HCC instances, segmented linear regression evaluation with two segments was carried out, and the interval for the early and late recurrence teams was partitioned on the crosspoint (676 days). We investigated whether or not gene expression in non-tumorous tissues of remnant liver from 39 hepatitis C virus-positive HCC instances could also be related to early recurrence of this illness.
By microarray evaluation, 21 genes have been recognized as candidate recurrence-associated genes. Additional gene expression evaluation was carried out, and the localization and expression of the gene merchandise of those candidate genes have been immunohistochemically evaluated. Low expression of the GBP1 gene and excessive expression of the TSC22D3 gene have been considerably (each P=0.04) related to the chance of early recurrence.
Via backward step-wise multivariate logistic regression evaluation for the 21 candidate genes, excessive expression of GBP1 lowered [odds ratio (OR)=0.20; 95% confidence interval (CI) 0.06-0.73, P=0.02] and excessive expression of TSC22D3 elevated the chance of early recurrence (OR=19.6; 95% CI 1.14-337.2; P=0.04).
Immunohistochemical evaluation revealed that hepatocytes confirmed sturdy membranous expression for GBP1 within the late recurrence group, however weak membranous expression for GBP1 within the early recurrence group. TSC22D3 was ceaselessly expressed in lymphocytes and in a couple of hepatocytes in tissues of the early recurrence group.
Our observations counsel that the mix of the excessive expression of the TSC22D3 gene and low expression of the GBP1 gene within the non-tumorous tissue of the remnant liver is considerably related to early recurrence after surgical resection of HCC.