[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]

Plasmid-mediated gene remedy, being a protected and comparatively cheap therapeutic technique, is stricken by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors. We have now earlier constructed a low-CpG pMBR2 plasmid vector supporting extended expression of transgenes in mesenchymal stem cells in vitro. Lengthy-term expression from the […]