Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells.

arf1 antibody, Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells.
Cell-matrix adhesion regulates membrane trafficking controlling anchorage-dependent signaling. Whereas a dynamic Golgi advanced can contribute to this pathway, its regulation by adhesion stays unclear. Right here we report that lack of adhesion dramatically disorganized the Golgi in mouse and human fibroblast cells. Golgi integrity is restored quickly upon integrin-mediated re-adhesion to FN and is disrupted by integrin blocking antibody.
In suspended cells, the cis, cis-medial and trans-Golgi networks differentially disorganize alongside the microtubule community however present no overlap with the ER, making this disorganization distinct from identified Golgi fragmentation. This pathway is regulated by an adhesion-dependent discount and restoration of Arf1 activation. Constitutively lively Arf1 disrupts this regulation and prevents Golgi disorganization because of lack of adhesion.
Adhesion-dependent Arf1 activation regulates its binding to the microtubule minus-end motor protein dynein to regulate Golgi reorganization, which is blocked by ciliobrevin. Adhesion-dependent Golgi group controls its operate, regulating cell floor glycosylation because of lack of adhesion, which is blocked by constitutively lively Arf1. This research, therefore, recognized integrin-dependent cell-matrix adhesion to be a novel regulator of Arf1 activation, controlling Golgi group and performance in anchorage-dependent cells. This text has an related First Individual interview with the primary writer of the paper.

Evaluation of Membrane Protein Topology within the Plant Secretory Pathway.

Topology of membrane proteins offers vital info for the understanding of protein operate and intermolecular associations. Combine membrane proteins are typically transported from endoplasmic reticulum (ER) to Golgi and downstream compartments within the plant secretory pathway. Right here, we describe a easy methodology to check membrane protein topology alongside the plant secretory pathway by transiently coexpressing a fluorescent protein (XFP)-tagged membrane protein and an ER export inhibitor protein, ARF1 (T31N), in tobacco BY-2 protoplast.
By fractionation, microsome isolation, and trypsin digestion, membrane protein topology may very well be simply detected by both direct confocal microscopy imaging or western-blot evaluation utilizing particular XFP antibodies. An analogous technique in figuring out membrane protein topology may very well be extensively adopted and utilized to protein evaluation in a broad vary of eukaryotic methods, together with yeast cells and mammalian cells.

Mixed concentrating on of Arf1 and Ras potentiates anticancer exercise for prostate most cancers therapeutics.

Though main enhancements have been made in surgical administration, chemotherapeutic, and radiotherapeutic of prostate most cancers, many prostate cancers stay refractory to therapy with customary brokers.
Subsequently, the identification of latest molecular targets in most cancers development and growth of novel therapeutic methods to focus on them are very needed for reaching higher survival for sufferers with prostate most cancers. Activation of small GTPases reminiscent of Ras and Arf1 is a crucial element of the signaling pathways for a lot of the receptors proven to be upregulated in superior prostate most cancers.

Which Approach In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.

Bacterial Sec7-domain-containing proteins (RalF) are identified solely from species of Legionella and Rickettsia, which have facultative and obligate intracellular existence, respectively. L. pneumophila RalF, a kind IV secretion system (T4SS) effector, is a guanine nucleotide trade issue (GEF) of ADP-ribosylation elements (Arfs), activating and recruiting host Arf1 to the Legionella-containing vacuole.
In distinction, earlier in vitro research confirmed R. prowazekii (Typhus Group) RalF is a practical Arf-GEF that localizes to the host plasma membrane and interacts with the actin cytoskeleton through a novel C-terminal area. As RalF is differentially encoded throughout Rickettsia species (e.g., pseudogenized in all Noticed Fever Group species), it could operate in lineage-specific biology and pathogenicity.
Herein, we display RalF of R. typhi (Typhus Group) interacts with the Rickettsia T4SS coupling protein (RvhD4) through its proximal C-terminal sequence. RalF is expressed early throughout an infection, with its inactivation through antibody blocking considerably decreasing R. typhi host cell invasion.
arf1 antibody, Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells.
For R. typhi and R. felis (Transitional Group), RalF ectopic expression revealed subcellular localization with the host plasma membrane and actin cytoskeleton. Remarkably, R. bellii (Ancestral Group) RalF confirmed perinuclear localization paying homage to ectopically expressed Legionella RalF, for which it shares a number of structural options. For R. typhi, RalF co-localization with Arf6 and PI(4,5)P2 at entry foci on the host plasma membrane was decided to be crucial for invasion.
Thus, we suggest recruitment of PI(4,5)P2 at entry foci, mediated by RalF activation of Arf6, initiates actin transforming and finally facilitates bacterial invasion. Collectively, our characterization of RalF as an invasin means that, regardless of carrying an identical Arf-GEF unknown from different micro organism, completely different intracellular existence throughout Rickettsia and Legionella species have pushed divergent roles for RalF throughout an infection. Moreover, our identification of lineage-specific Arf-GEF utilization throughout some rickettsial species illustrates completely different pathogenicity elements that outline numerous brokers of rickettsial ailments.

Hematopoietic stem/progenitor cells immediately contribute to arteriosclerotic development through integrin β2.

Current research described the affiliation between hematopoietic stem/progenitor cell (HSPC) enlargement within the bone marrow (BM), leukocytosis within the peripheral blood, and accelerated atherosclerosis. We hypothesized that circulating HSPC could house to infected vessels, the place they may contribute to irritation and neointima formation.
We demonstrated that Lin(-) Sca-1(+) cKit(+) (LSK cells) in BM and peripheral blood of LDLr(-/-) mice on excessive fats eating regimen expressed considerably extra integrin β2 , which was answerable for LSK cell adhesion and migration towards ICAM-1 in vitro, and homing to injured arteries in vivo, all of which have been blocked with an anti-CD18 blocking antibody. When homed LSK cells have been remoted from ligated artery and injected to irradiated recipients, they resulted in BM reconstitution.
Injection of CD18(+/+) LSK cells to immunodeficient Balb/C Rag2(-) ɣC(-/-) recipients resulted in additional extreme irritation and strengthened neointima formation within the ligated carotid artery, in comparison with mice injected with PBS and CD18(-/-) LSK cells. Hypercholesterolemia stimulated ERK phosphorylation (pERK) in LSK cells of LDLr(-/-) mice in vivo.

ARF1 antibody

70R-12842 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal ARF1 antibody

ARF1 antibody

70R-13531 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal ARF1 antibody

ARF1 antibody

70R-15789 50 ul
EUR 435
Description: Rabbit polyclonal ARF1 antibody

ARF1 antibody

10R-1775 100 ul
EUR 322
Description: Mouse monoclonal ARF1 antibody

ARF1 antibody

10R-1783 100 ul
EUR 322
Description: Mouse monoclonal ARF1 antibody

ARF1 antibody

10-2473 250 ug
EUR 492
Description: Mouse monoclonal ARF1 antibody

ARF1 Antibody

49120-100ul 100ul
EUR 333

ARF1 Antibody

49120-50ul 50ul
EUR 239

ARF1 Antibody

1-CSB-PA001988GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity Purified
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

ARF1 Antibody

1-CSB-PA957575
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

ARF1 Antibody

DF7650 200ul
EUR 304
Description: ARF1 Antibody detects endogenous levels of total ARF1.

ARF1 antibody

70R-8783 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal ARF1 antibody

ARF1 antibody

70R-5876 50 ug
EUR 467
Description: Rabbit polyclonal ARF1 antibody raised against the middle region of ARF1

ARF1 Antibody

1-CSB-PA00929A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

ARF1 Antibody

ABD7650 100 ug
EUR 438

ARF1 Antibody

ABD8122 100 ug
EUR 438

ARF1 Conjugated Antibody

C49120 100ul
EUR 397

ARF1 Conjugated Antibody

C31145 100ul
EUR 397

anti- ARF1 antibody

FNab00528 100µg
EUR 505.25
  • Immunogen: ADP-ribosylation factor 1
  • Uniprot ID: P84077
  • Gene ID: 375
  • Research Area: Metabolism
Description: Antibody raised against ARF1

anti- ARF1 antibody

FNab00529 100µg
EUR 548.75
  • Immunogen: ADP-ribosylation factor 1
  • Uniprot ID: P84077
  • Gene ID: 375
  • Research Area: Metabolism
Description: Antibody raised against ARF1

Anti-ARF1 antibody

PAab00528 100 ug
EUR 355

Anti-ARF1 antibody

PAab00529 100 ug
EUR 386

Anti-ARF1 antibody

STJ11100853 100 µl
EUR 413
Description: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Anti-ARF1 antibody

STJ113570 100 µl
EUR 277
Description: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Anti-ARF1 antibody

STJ118785 100 µl
EUR 277

Anti-ARF1 Antibody

STJ500153 100 µg
EUR 515

Anti-ARF1 antibody

STJ22666 100 µl
EUR 277
Description: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Arf1/ Rat Arf1 ELISA Kit

ELI-11213r 96 Tests
EUR 886

ARF1 siRNA

20-abx900400
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

ARF1 siRNA

20-abx907921
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

ARF1 siRNA

20-abx907922
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

anti-ARF1

YF-PA23239 50 ul
EUR 334
Description: Mouse polyclonal to ARF1

Polyclonal ARF1 Antibody (Center)

APR15007G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ARF1 (Center). This antibody is tested and proven to work in the following applications:

ARF1 Antibody, HRP conjugated

1-CSB-PA00929B0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ARF1 Antibody, FITC conjugated

1-CSB-PA00929C0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ARF1 Antibody, Biotin conjugated

1-CSB-PA00929D0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ARF1. Recognizes ARF1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Anti-ARF1 Antibody BIOTIN

STJ500154 100 µg
EUR 586

Anti-ARF1 Antibody FITC

STJ500155 100 µg
EUR 586

ARF1 Rabbit pAb

A16345-100ul 100 ul
EUR 308

ARF1 Rabbit pAb

A16345-200ul 200 ul
EUR 459

ARF1 Rabbit pAb

A16345-20ul 20 ul
EUR 183

ARF1 Rabbit pAb

A16345-50ul 50 ul
EUR 223

ARF1 Blocking Peptide

33R-4444 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ARF1 antibody, catalog no. 70R-8783

ARF1 Blocking Peptide

33R-6371 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ARF1 antibody, catalog no. 70R-5876
Blockade of pERK diminished ARF1 expression, resulting in decreased integrin β2 operate on HSPC. As well as, integrin β2 operate may very well be regulated through ERK-independent LRP1 pathway. Integrin β2 expression on HSPC is regulated by hypercholesterolemia, particularly LDL, in pERK-dependent and -independent manners, resulting in elevated homing and localization of HSPC to injured arteries, which is very correlated with arteriosclerosis.

Leave a Reply

Your email address will not be published. Required fields are marked *

Related Post

Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury

Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injuryAdministration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury

The results of cyclophosphamide on 5-hydroxytryptamine (5-HT) synthesis within the intestinal tissue of rats have been investigated. Rats acquired 120 mg/kg cyclophosphamide intraperitoneally as a single administration, and kaolin and