Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those plasmids from their bacterial cells is a vital step in figuring out strain-specific virulence traits and to assemble strains helpful for transformation. Right here, we developed two highly effective instruments to enhance this course of. We first established a reporter system to detect the presence and absence of Ti/Ri plasmids in cells by utilizing an acetosyringone (AS)-inducible promoter of the Ti2 small RNA and luxAB from Vibrio harveyi. This technique distinguished a Ti/Ri plasmid-free cell colony amongst plasmid-harboring cell colonies by inflicting the latter colonies to emit gentle in response to AS.
We then constructed new “Ti/Ri eviction plasmids,” every of which carries a repABC from certainly one of 4 Ti/Ri plasmids that belonged to incRh1, incRh2, incRh3, and incRh4 teams within the suicidal plasmid pK18mobsacB and in a broad-host-range pBBR1 vector. Introduction of the brand new eviction plasmids into Agrobacterium cells harboring the corresponding Ti/Ri plasmids led to Ti/Ri plasmid-free cells in each incRh group. The Ti/Ri eviction was simpler by plasmids with the pBBR1 spine than by these with the pK18mobsacB spine. Moreover, the extremely steady cryptic plasmid pAtC58 in A. tumefaciens C58 was successfully evicted by the introduction of a pBBR1 vector containing the repABC of pAtC58. These outcomes point out that the set of pBBR1-repABC plasmids is a strong device for the removing of steady rhizobial plasmids.
Evaluation of UV spectrophotometry for dedication of plasmid DNA focus in vector preparations for human gene remedy merchandise.
The European Pharmacopoeia (Ph. Eur.) normal chapter 5.14. Gene switch medicinal merchandise for human use suggests the usage of absorbance measurements at 260 nm to find out the DNA focus of plasmid vectors used for the preparation of gene remedy merchandise for human use. A global collaborative examine was organised by the European Directorate for the High quality of Medicines & HealthCare (EDQM) to verify the suitability of UV spectrophotometry for the quantification of plasmid vectors utilized in gene remedy (GT).
Three Official Drugs Management Laboratories (OMCLs of the European OMCL Community) and members of the OMCL Working Group for GT merchandise took half within the examine, during which varied kinds of spectrophotometers have been assessed utilizing widespread take a look at samples. Outcomes of the examine demonstrated that UV spectrophotometry may be thought-about appropriate for the quantification of plasmid DNA in GT merchandise whatever the instrument used.
All formulations confirmed low vital micelle focus (CMC) values meaning extremely steady in serum containing medium. Polymeric micelles have been additionally evaluated for his or her stability within the presence of serum and nuclease in addition to cytotoxicity and transfection effectivity. All our outcomes proved that our novel polymeric micellar system ready by PPP60 block copolymer provide to be an environment friendly promising provider for gene supply functions. Furthermore, these findings contribute to design and improvement of novel gene vectors with tunable and performance options and in addition to cut back the cytotoxicity of PEI by partial hydrolysis of PEtOx an alternate synthesis methodology to provide linear PEI.
Plasmid-Templated Management of DNA-Cyclodextrin Nanoparticle Morphology by Molecular Vector Design for Efficient Gene Supply.
Engineering self-assembled superstructures by complexation of plasmid DNA (pDNA) and single-isomer nanometric measurement macromolecules (molecular nanoparticles) is a promising technique for gene supply. Notably, the performance and general structure of the vector may be exactly molded on the atomic stage by chemical tailoring, thereby enabling unprecedented alternatives for construction/self-assembling/pDNA supply relationship research. The experimental findings are in settlement with a shift from chelate to cross-linking interactions on going from primary-face- to secondary-face-linked CD dimers, the pDNA accomplice appearing as an energetic payload and as a template.
Past this notion, by judiciously preorganizing the purposeful parts in cyclodextrin (CD)-based molecular nanoparticles by covalent dimerization, right here we display that the morphology of the ensuing nanocomplexes (CDplexes) may be tuned, from spherical to ellipsoidal, rod-type, or worm-like nanoparticles, which makes it doable to achieve understanding of their shape-dependent transfection properties. Most curiously, the transfection effectivity in numerous cells was proven to be in a different way impacted by modifications of the CDplex morphology, which has led to the identification of an optimum prototype for tissue-selective DNA supply to the spleen in vivo.
Persistence of plasmid-mediated expression of transgenes in human mesenchymal stem cells relies upon totally on CpG ranges of each vector and transgene.
Gene remedy and cell modification for medical functions utilizing plasmid vectors are thought-about to be a protected and promising technique. One of many main issues with plasmid vector-based constructs is a speedy decline of transgene expression in cells in vitro and in vivo. An vital position of CpG motifs or bacterial vector spine in expression silencing has been recommended. The pMBR2 vector with CpG-free luciferase insert demonstrated the very best persistence of expression, whereas the wild-type luciferase insert containing 97 CpG motifs demonstrated decrease expression upkeep in the identical vector.
) SYBR Green qPCR Master Mix (No ROX) |
|
C0006-1mg |
TargetMol Chemicals |
1mg |
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|
Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
|
C0006-50mg |
TargetMol Chemicals |
50mg |
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|
Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
|
C0006-5mg |
TargetMol Chemicals |
5mg |
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|
Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
|
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (Low ROX) |
|
C0008-10mg |
TargetMol Chemicals |
10mg |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
C0008-1g |
TargetMol Chemicals |
1g |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
C0008-1mg |
TargetMol Chemicals |
1mg |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
C0008-50mg |
TargetMol Chemicals |
50mg |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
C0008-5mg |
TargetMol Chemicals |
5mg |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (High ROX) |
|
C0007-10mg |
TargetMol Chemicals |
10mg |
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|
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
C0007-1g |
TargetMol Chemicals |
1g |
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|
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
C0007-1mg |
TargetMol Chemicals |
1mg |
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|
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
C0007-50mg |
TargetMol Chemicals |
50mg |
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|
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
C0007-5mg |
TargetMol Chemicals |
5mg |
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|
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
) 2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21702 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) 2x SYBR Green qPCR Master Mix (High ROX) |
|
B21402 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
|
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
 AceQ qPCR SYBR® Green Master Mix |
|
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
|
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
 Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
|
THK0221 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
|
THK0222 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0223 |
Westburg |
each |
EUR 3324.5 |
 Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
|
THK0241 |
Westburg |
each |
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 Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
|
THK0242 |
Westburg |
each |
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 Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0243 |
Westburg |
each |
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 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
|
THK0251 |
Westburg |
each |
EUR 232.17 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
|
THK0252 |
Westburg |
each |
EUR 1046.4 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
|
THK0253 |
Westburg |
each |
Ask for price |
) BioEasy Master Mix (SYBR Green) |
|
TRI-B25L1 |
TRI Biotech |
200T |
EUR 403.2 |
 SYBR Green cRNA PCR Master Mix |
|
SGM002 |
Signosis |
2 ml |
EUR 174 |
 EnoGene Green qPCR Master Mix |
|
E31M001 |
EnoGene |
500 reaction |
EUR 75 |
 ChamQ SYBR qPCR Master Mix |
|
Q311-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 Apex qPCR GREEN Master Mix |
|
42-116PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, Without ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-117PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Without ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-118PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 338.88 |
|
Description: 2X, Low ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-119PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Low ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-120PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, High ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-121PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 3095.57 |
|
Description: 2X, High ROX, 50ml |
) BioEasy Master Mix (SYBR Green, No ROX) |
|
TRI-B25L1D |
TRI Biotech |
200T |
EUR 403.2 |
) BioEasy Master Mix (SYBR Green, low ROX) |
|
TRI-B25L1C |
TRI Biotech |
200T |
EUR 403.2 |
) BioEasy Master Mix (SYBR Green, high ROX) |
|
TRI-B25L1B |
TRI Biotech |
200T |
EUR 403.2 |
 ChamQ SYBR Color qPCR Master Mix |
|
Q411-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 AceQ Universal SYBR qPCR Master Mix |
|
Q511-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 miRNA Universal SYBR qPCR Master Mix |
|
MQ101-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01-125rxns |
Vazyme |
125 rxns |
EUR 40.28 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 145 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02-500rxns |
Vazyme |
500 rxns |
EUR 150.15 |
 ChamQ Universal SYBR qPCR Master Mix |
|
Q711-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 HotStart™ 2X Green qPCR Master Mix |
|
K1070-25 |
ApexBio |
25ml |
EUR 392 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-5 |
ApexBio |
5ml |
EUR 124 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-50 |
ApexBio |
50ml |
EUR 768 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
 2×SYBR Green qPCR Mix |
|
E37F021-10x5mL |
EnoGene |
10×5mL |
EUR 1360 |
|
Description: DsDNA, ssDNA, RNA quantitative detection kit |
2×SYBR Green qPCR Mix |
|
E37F021-5mL |
EnoGene |
5mL |
EUR 227 |
|
Description: Fluorescent quantitative PCR kits |
2×SYBR Green qPCR Mix |
|
MBS8579840-200Tests |
MyBiosource |
200Tests |
EUR 285 |
2×SYBR Green qPCR Mix |
|
MBS8579840-50Tests |
MyBiosource |
50Tests |
EUR 180 |
2×SYBR Green qPCR Mix |
|
MBS8579840-5x200Tests |
MyBiosource |
5x200Tests |
EUR 1230 |
2×SYBR Green qPCR Mix |
|
MBS8579840-5x50Tests |
MyBiosource |
5x50Tests |
EUR 770 |
) ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 161 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-02-500rxns |
Vazyme |
500 rxns |
EUR 166.72 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 725 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 750.74 |
 miRNA SYBR Green qPCR Mix |
|
4308A |
Unibiotest |
100 Tests*20μL |
EUR 80 |
miRNA SYBR Green qPCR Mix |
|
4308B |
Unibiotest |
200 Tests*20μL |
EUR 150 |
miRNA SYBR Green qPCR Mix |
|
4308C |
Unibiotest |
500 Tests*20μL |
EUR 300 |
, 100rxns) ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
|
QLMM10 |
Vivantis |
each |
Ask for price |
 HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
 HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-25 |
ApexBio |
25ml |
EUR 392 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-5 |
ApexBio |
5ml |
EUR 124 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-50 |
ApexBio |
50ml |
EUR 752 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
) ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
) ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
) ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
 ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
|
QLMM12 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
|
QLMM17 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
|
QLMM02 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns |
|
QLMM07 |
Vivantis |
each |
Ask for price |
) ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
) ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 ABsolute SYBR Green QPCR Mix 800 rxns |
|
AB-1158/B |
Westburg |
each |
Ask for price |
 ABsolute SYBR Green QPCR Mix 200 rxns |
|
AB-1159 |
Westburg |
each |
EUR 529.74 |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17272 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17273 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17274 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17275 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
) 2×SYBR Green qPCR Mix(including ROX) |
|
E37F022-10x5mL |
EnoGene |
10×5mL |
EUR 1360 |
|
Description: Fluorescent quantitative PCR kits |
2×SYBR Green qPCR Mix(including ROX) |
|
E37F022-5mL |
EnoGene |
5mL |
EUR 227 |
|
Description: Fluorescent quantitative PCR kits |
) 2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-200Tests |
MyBiosource |
200Tests |
EUR 285 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-50Tests |
MyBiosource |
50Tests |
EUR 180 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-5x200Tests |
MyBiosource |
5x200Tests |
EUR 1230 |
2×SYBR Green qPCR Mix (including ROX) |
|
MBS8579841-5x50Tests |
MyBiosource |
5x50Tests |
EUR 770 |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17276 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17277 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 Genious 2X SYBR Green Fast qPCR Mix |
|
RK21204 |
Abclonal |
1 mL |
EUR 76.3 |
|
|
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17278 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17279 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17280 |
AAT Bioquest |
1 mL |
EUR 203 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17281 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 2X Universal SYBR Green Fast qPCR Mix |
|
MBS9141672-25mL |
MyBiosource |
25mL |
EUR 755 |
2X Universal SYBR Green Fast qPCR Mix |
|
MBS9141672-5mL |
MyBiosource |
5mL |
EUR 255 |
 2X Universal SYBR Green Fast qPCR Mix |
|
RK21203 |
Abclonal |
1 mL |
EUR 21.26 |
|
|
) 2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302A |
Unibiotest |
1 mL |
EUR 80 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302B |
Unibiotest |
5*1 mL |
EUR 250 |
2 x SYBR Green qPCR Mix(Antibody，ROX) |
|
4302C |
Unibiotest |
50*1 mL |
EUR 1400 |
Compared, the identical inserts within the CpG-replete pCDNA3 vector demonstrated considerably decrease expression ranges and solely a minimal persistence of expression. β-galactosidase and enhanced inexperienced fluorescent protein genes inserted into pMBR2 vector additionally demonstrated increased expression ranges and higher upkeep in comparison with the identical genes in pCDNA3 vector.
