Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those plasmids from their bacterial cells is a vital step in figuring out strain-specific virulence traits and to assemble strains helpful for transformation. Right here, we developed two highly effective instruments to enhance this course of. We first established a reporter system to detect the presence and absence of Ti/Ri plasmids in cells by utilizing an acetosyringone (AS)-inducible promoter of the Ti2 small RNA and luxAB from Vibrio harveyi. This technique distinguished a Ti/Ri plasmid-free cell colony amongst plasmid-harboring cell colonies by inflicting the latter colonies to emit gentle in response to AS.

We then constructed new “Ti/Ri eviction plasmids,” every of which carries a repABC from certainly one of 4 Ti/Ri plasmids that belonged to incRh1, incRh2, incRh3, and incRh4 teams within the suicidal plasmid pK18mobsacB and in a broad-host-range pBBR1 vector. Introduction of the brand new eviction plasmids into Agrobacterium cells harboring the corresponding Ti/Ri plasmids led to Ti/Ri plasmid-free cells in each incRh group. The Ti/Ri eviction was simpler by plasmids with the pBBR1 spine than by these with the pK18mobsacB spine. Moreover, the extremely steady cryptic plasmid pAtC58 in A. tumefaciens C58 was successfully evicted by the introduction of a pBBR1 vector containing the repABC of pAtC58. These outcomes point out that the set of pBBR1-repABC plasmids is a strong device for the removing of steady rhizobial plasmids.

Evaluation of UV spectrophotometry for dedication of plasmid DNA focus in vector preparations for human gene remedy merchandise.

The European Pharmacopoeia (Ph. Eur.) normal chapter 5.14. Gene switch medicinal merchandise for human use suggests the usage of absorbance measurements at 260 nm to find out the DNA focus of plasmid vectors used for the preparation of gene remedy merchandise for human use. A global collaborative examine was organised by the European Directorate for the High quality of Medicines & HealthCare (EDQM) to verify the suitability of UV spectrophotometry for the quantification of plasmid vectors utilized in gene remedy (GT).

Three Official Drugs Management Laboratories (OMCLs of the European OMCL Community) and members of the OMCL Working Group for GT merchandise took half within the examine, during which varied kinds of spectrophotometers have been assessed utilizing widespread take a look at samples. Outcomes of the examine demonstrated that UV spectrophotometry may be thought-about appropriate for the quantification of plasmid DNA in GT merchandise whatever the instrument used.

All formulations confirmed low vital micelle focus (CMC) values meaning extremely steady in serum containing medium. Polymeric micelles have been additionally evaluated for his or her stability within the presence of serum and nuclease in addition to cytotoxicity and transfection effectivity. All our outcomes proved that our novel polymeric micellar system ready by PPP60 block copolymer provide to be an environment friendly promising provider for gene supply functions. Furthermore, these findings contribute to design and improvement of novel gene vectors with tunable and performance options and in addition to cut back the cytotoxicity of PEI by partial hydrolysis of PEtOx an alternate synthesis methodology to provide linear PEI.

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Plasmid-Templated Management of DNA-Cyclodextrin Nanoparticle Morphology by Molecular Vector Design for Efficient Gene Supply.

Engineering self-assembled superstructures by complexation of plasmid DNA (pDNA) and single-isomer nanometric measurement macromolecules (molecular nanoparticles) is a promising technique for gene supply. Notably, the performance and general structure of the vector may be exactly molded on the atomic stage by chemical tailoring, thereby enabling unprecedented alternatives for construction/self-assembling/pDNA supply relationship research. The experimental findings are in settlement with a shift from chelate to cross-linking interactions on going from primary-face- to secondary-face-linked CD dimers, the pDNA accomplice appearing as an energetic payload and as a template.

Past this notion, by judiciously preorganizing the purposeful parts in cyclodextrin (CD)-based molecular nanoparticles by covalent dimerization, right here we display that the morphology of the ensuing nanocomplexes (CDplexes) may be tuned, from spherical to ellipsoidal, rod-type, or worm-like nanoparticles, which makes it doable to achieve understanding of their shape-dependent transfection properties. Most curiously, the transfection effectivity in numerous cells was proven to be in a different way impacted by modifications of the CDplex morphology, which has led to the identification of an optimum prototype for tissue-selective DNA supply to the spleen in vivo.

Persistence of plasmid-mediated expression of transgenes in human mesenchymal stem cells relies upon totally on CpG ranges of each vector and transgene.

Gene remedy and cell modification for medical functions utilizing plasmid vectors are thought-about to be a protected and promising technique. One of many main issues with plasmid vector-based constructs is a speedy decline of transgene expression in cells in vitro and in vivo. An vital position of CpG motifs or bacterial vector spine in expression silencing has been recommended. The pMBR2 vector with CpG-free luciferase insert demonstrated the very best persistence of expression, whereas the wild-type luciferase insert containing 97 CpG motifs demonstrated decrease expression upkeep in the identical vector.

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

W2601-5 NULL
EUR 0

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 113

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 257

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 257

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 646

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 224
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 856
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 224
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 856
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

ig SYBR Green qPCR 2X Master Mix - 500 Reactions

3356 1/EA
EUR 288

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 221

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 646

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 221

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 646

miRNA Universal SYBR® qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 138

miRNA Universal SYBR® qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 242

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 646

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 1ml
EUR 280

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 2x1ml
EUR 482

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 5x1ml
EUR 828

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 10x1ml
EUR 1360

HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
EUR 209.5
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 96.4
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

RT Master Mix for qPCR (gDNA digester plus)

HY-K0511 1 mL (100 rxns)
EUR 315

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 2x1ML
EUR 204
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 5x1ML
EUR 386
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3116
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)

31020 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)

31020-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)

31020-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

SYBR Green I

B1747-25
EUR 1164

SYBR Green I

B1747-5
EUR 387

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)

31041-1 5x1mL
EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)

31041-20mL 2x10mL
EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)

31041-T 1mL
EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Green qPCR SuperMix

20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

EZSolution? SYBR Green I

B1748-1
EUR 620

EZSolution? SYBR Green I

B1748-500
EUR 430

Jade? Master Mix

M1105-500
EUR 441

Taqman Master Mix

M1121-500
EUR 441

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)

31003-T 1ML
EUR 123
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)

31020-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Green Taq Mix

P131-01 5 ml
EUR 119

Green Taq Mix

P131-02 15 ml
EUR 150

Green Taq Mix

P131-03 50 ml
EUR 248

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)

31014 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)

31014-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

31014-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)

31015-1 5x1mL
EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)

31015-2 50x1mL
EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)

31042-1 5x1mL
EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)

31042-20mL 2x10mL
EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)

31042-T 1mL
EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)

9-31045
  • EUR 114.00
  • EUR 482.00
  • EUR 266.00
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)

9-31046
  • EUR 114.00
  • EUR 482.00
  • EUR 266.00
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190L 500 rcs (12,5 ml)
EUR 523

2 × Taq Master Mix

P111-01 5 ml
EUR 117

2 × Taq Master Mix

P111-02 15 ml
EUR 146

2 × Taq Master Mix

P111-03 50 ml
EUR 236

Accuris Taq Master Mix

PR1001-1000 1 PC
EUR 300.26
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Master Mix

PR1001-200 1 PC
EUR 121.62
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Master Mix

PR1001-S 1 PC
EUR 69.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Taqman Master Mix-iCycler

M1122-500
EUR 441

Taqman Master Mix-ROX

M1124-500
EUR 441

Taqman Master Mix-Multiplex

M1125-500
EUR 441

amfiSure PCR Master Mix

P0311-010 2x50 rxns
EUR 126

amfiSure PCR Master Mix

P0311-025 5X50 rxns
EUR 165

amfiSure PCR Master Mix

P0311-050 10X50 rxns
EUR 219

amfiSure PCR Master Mix

P0311-100 10x1ml
EUR 286

amfiSure PCR Master Mix

P0311-125 15X50 rxns
EUR 278

amfiSure PCR Master Mix

P0311-200 20x1ml
EUR 469

amfiSure PCR Master Mix

P0311-250 50x50 rxns
EUR 752

amfiSure PCR Master Mix

P0311-500 100x50 rxns
EUR 1258

amfiXpand PCR Master Mix

P0331-010 2X50 rxns
EUR 151

amfiXpand PCR Master Mix

P0331-025 5X50 rxns
EUR 273

amfiXpand PCR Master Mix

P0331-050 10X50 rxns
EUR 448

amfiXpand PCR Master Mix

P0331-250 50x50 rxns
EUR 1873

2 × AceTaq Master Mix

P411-01 1 ml
EUR 119

2 × AceTaq Master Mix

P411-02 5 ml
EUR 158

2 × AceTaq Master Mix

P411-03 15 ml
EUR 262

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)

31014-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)

31015 2x1mL
EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)

31015-T 1mL
EUR 134
Description: Minimum order quantity: 1 unit of 1mL

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials

S150L 500 rcs (12,5 ml)
EUR 523

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials

S200 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials

S200L 500 rcs (12,5 ml)
EUR 612

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

amfiSure Prime PCR Master Mix

P1311-025 5X50 rxns
EUR 161

amfiSure Prime PCR Master Mix

P1311-050 10X50 rxns
EUR 219

amfiSure Prime PCR Master Mix

P1311-125 15X50 rxns
EUR 273

amfiSure Prime PCR Master Mix

P1311-250 50x50 rxns
EUR 739

amfiSure Prime PCR Master Mix

P1311-500 100x50 rxns
EUR 1238

2 × Taq Plus Master Mix

P211-01 5 ml
EUR 134

2 × Taq Plus Master Mix

P211-02 15 ml
EUR 193

2 × Taq Plus Master Mix

P211-03 50 ml
EUR 389

Accuris Taq Plus Master Mix

PR1001-TP-1000 1 PC
EUR 827.41
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Plus Master Mix

PR1001-TP-200 1 PC
EUR 242.84
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Plus Master Mix

PR1001-TP-S 1 PC
EUR 69.79
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Taqman Master Mix-Low ROX

M1123-500
EUR 441

2 × Rapid Taq Master Mix

P222-01 5 ml (5×1ml)
EUR 120

2 × Rapid Taq Master Mix

P222-02 15 ml (15×1ml)
EUR 153

2 × Rapid Taq Master Mix

P222-03 50 ml (50 x 1 ml)
EUR 257

2 × Rapid Taq Master Mix

P222-04 50 ml (10×5ml)
EUR 257

amfiSure Advanced PCR Master Mix

P2311-025 5X50 rxns
EUR 165

amfiSure Advanced PCR Master Mix

P2311-050 10X50 rxns
EUR 223

amfiSure Advanced PCR Master Mix

P2311-125 15X50 rxns
EUR 282

amfiSure Advanced PCR Master Mix

P2311-250 50x50 rxns
EUR 752

amfiSure Advanced PCR Master Mix

P2311-500 100x50 rxns
EUR 1279

2 × Vazyme LAmp Master Mix

P311-01 1 ml
EUR 137

2 × Vazyme LAmp Master Mix

P311-02 5 ml
EUR 212

2 × Vazyme LAmp Master Mix

P311-03 15 ml
EUR 401

2 × Phanta Max Master Mix

P515-01 1 ml
EUR 145

2 × Phanta Max Master Mix

P515-02 5 ml
EUR 269

2 × Phanta Max Master Mix

P515-03 15 ml
EUR 575

AceQ U+ Probe Master Mix

Q113-02 500 rxn (20 μl/rxn)
EUR 267

AceQ U+ Probe Master Mix

Q113-03 2500 rxn (20 μl/rxn)
EUR 842

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

Seamless cloning Master Mix (Kit)

B632219 40RXN, 40prep
EUR 266.63
  • Product category: Molecular Biology Kits/Cloning/Cloning Kits (Flexible)

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials

S160 100 rcs (2,5 ml)
EUR 159

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials

S160L 500 rcs (12,5 ml)
EUR 612

SYBR Green I Nucleic Acid Gel Stain

HY-K1004 100 μL
EUR 113

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

SensiFAST SYBR Lo-ROX Mix, 50 mL

BIO-94050 5000 rxn Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL

BIO-98050 5000 rxn Ask for price

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

HotTaq Probe qPCR Mix (no ROX)

BT11002 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)

BT11102 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials

S210 100 rcs (2,5 ml)
EUR 138

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials

S210L 500 rcs (12,5 ml)
EUR 523

ExoELISA-ULTRA Complete Kit (CD63 detection)

EXEL-ULTRA-CD63-1 96 reactions
EUR 713
  • Category: Exosomes

ExoELISA-ULTRA Complete Kit (CD81 detection)

EXEL-ULTRA-CD81-1 96 reactions
EUR 713
  • Category: Exosomes

2× EpiArt HS Taq Master Mix

EM201-01 1ml
EUR 145

2× EpiArt HS Taq Master Mix

EM201-02 5 ml (5×1ml)
EUR 303

2× EpiArt HS Taq Master Mix

EM201-03 15 ml (15×1ml)
EUR 642

Fast Probe Master Mix (200 rxn)

31005 2x1ML
EUR 234
Description: Minimum order quantity: 1 unit of 2x1ML

Fast Probe Master Mix (500 rxn)

31005-1 5x1ML
EUR 466
Description: Minimum order quantity: 1 unit of 5x1ML

Fast Probe Master Mix (5000 rxn)

31005-2 50x1ML
EUR 3871
Description: Minimum order quantity: 1 unit of 50x1ML

Pfu 2x Master Mix - 200 Reactions

3326 1/EA
EUR 300

2 × Taq Master Mix (Dye Plus)

P112-01 5 ml
EUR 117

2 × Taq Master Mix (Dye Plus)

P112-02 15 ml
EUR 146

2 × Taq Master Mix (Dye Plus)

P112-03 50 ml
EUR 236

Accuris Hot Start Taq Master Mix

PR1001-HS-1000 1 PC
EUR 490.87
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Hot Start Taq Master Mix

PR1001-HS-200 1 PC
EUR 169.47
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Master Mix Red Dye

PR1001-R-1000 1 PC
EUR 300.26
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris Taq Master Mix Red Dye

PR1001-R-200 1 PC
EUR 121.62
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Compared, the identical inserts within the CpG-replete pCDNA3 vector demonstrated considerably decrease expression ranges and solely a minimal persistence of expression. β-galactosidase and enhanced inexperienced fluorescent protein genes inserted into pMBR2 vector additionally demonstrated increased expression ranges and higher upkeep in comparison with the identical genes in pCDNA3 vector.

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