Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those plasmids from their bacterial cells is a vital step in figuring out strain-specific virulence traits and to assemble strains helpful for transformation. Right here, we developed two highly effective instruments to enhance this course of. We first established a reporter system to detect the presence and absence of Ti/Ri plasmids in cells by utilizing an acetosyringone (AS)-inducible promoter of the Ti2 small RNA and luxAB from Vibrio harveyi. This technique distinguished a Ti/Ri plasmid-free cell colony amongst plasmid-harboring cell colonies by inflicting the latter colonies to emit gentle in response to AS.

We then constructed new “Ti/Ri eviction plasmids,” every of which carries a repABC from certainly one of 4 Ti/Ri plasmids that belonged to incRh1, incRh2, incRh3, and incRh4 teams within the suicidal plasmid pK18mobsacB and in a broad-host-range pBBR1 vector. Introduction of the brand new eviction plasmids into Agrobacterium cells harboring the corresponding Ti/Ri plasmids led to Ti/Ri plasmid-free cells in each incRh group. The Ti/Ri eviction was simpler by plasmids with the pBBR1 spine than by these with the pK18mobsacB spine. Moreover, the extremely steady cryptic plasmid pAtC58 in A. tumefaciens C58 was successfully evicted by the introduction of a pBBR1 vector containing the repABC of pAtC58. These outcomes point out that the set of pBBR1-repABC plasmids is a strong device for the removing of steady rhizobial plasmids.

Evaluation of UV spectrophotometry for dedication of plasmid DNA focus in vector preparations for human gene remedy merchandise.

The European Pharmacopoeia (Ph. Eur.) normal chapter 5.14. Gene switch medicinal merchandise for human use suggests the usage of absorbance measurements at 260 nm to find out the DNA focus of plasmid vectors used for the preparation of gene remedy merchandise for human use. A global collaborative examine was organised by the European Directorate for the High quality of Medicines & HealthCare (EDQM) to verify the suitability of UV spectrophotometry for the quantification of plasmid vectors utilized in gene remedy (GT).

Three Official Drugs Management Laboratories (OMCLs of the European OMCL Community) and members of the OMCL Working Group for GT merchandise took half within the examine, during which varied kinds of spectrophotometers have been assessed utilizing widespread take a look at samples. Outcomes of the examine demonstrated that UV spectrophotometry may be thought-about appropriate for the quantification of plasmid DNA in GT merchandise whatever the instrument used.

All formulations confirmed low vital micelle focus (CMC) values meaning extremely steady in serum containing medium. Polymeric micelles have been additionally evaluated for his or her stability within the presence of serum and nuclease in addition to cytotoxicity and transfection effectivity. All our outcomes proved that our novel polymeric micellar system ready by PPP60 block copolymer provide to be an environment friendly promising provider for gene supply functions. Furthermore, these findings contribute to design and improvement of novel gene vectors with tunable and performance options and in addition to cut back the cytotoxicity of PEI by partial hydrolysis of PEtOx an alternate synthesis methodology to provide linear PEI.

Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.

Plasmid-Templated Management of DNA-Cyclodextrin Nanoparticle Morphology by Molecular Vector Design for Efficient Gene Supply.

Engineering self-assembled superstructures by complexation of plasmid DNA (pDNA) and single-isomer nanometric measurement macromolecules (molecular nanoparticles) is a promising technique for gene supply. Notably, the performance and general structure of the vector may be exactly molded on the atomic stage by chemical tailoring, thereby enabling unprecedented alternatives for construction/self-assembling/pDNA supply relationship research. The experimental findings are in settlement with a shift from chelate to cross-linking interactions on going from primary-face- to secondary-face-linked CD dimers, the pDNA accomplice appearing as an energetic payload and as a template.

Past this notion, by judiciously preorganizing the purposeful parts in cyclodextrin (CD)-based molecular nanoparticles by covalent dimerization, right here we display that the morphology of the ensuing nanocomplexes (CDplexes) may be tuned, from spherical to ellipsoidal, rod-type, or worm-like nanoparticles, which makes it doable to achieve understanding of their shape-dependent transfection properties. Most curiously, the transfection effectivity in numerous cells was proven to be in a different way impacted by modifications of the CDplex morphology, which has led to the identification of an optimum prototype for tissue-selective DNA supply to the spleen in vivo.

Persistence of plasmid-mediated expression of transgenes in human mesenchymal stem cells relies upon totally on CpG ranges of each vector and transgene.

Gene remedy and cell modification for medical functions utilizing plasmid vectors are thought-about to be a protected and promising technique. One of many main issues with plasmid vector-based constructs is a speedy decline of transgene expression in cells in vitro and in vivo. An vital position of CpG motifs or bacterial vector spine in expression silencing has been recommended. The pMBR2 vector with CpG-free luciferase insert demonstrated the very best persistence of expression, whereas the wild-type luciferase insert containing 97 CpG motifs demonstrated decrease expression upkeep in the identical vector.

SYBR Green qPCR Master Mix (No ROX)

C0006-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)

C0008-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)

C0007-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-25 25ml
EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-5 5ml
EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-50 50ml
EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions

3354 200
EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-25 25ml
EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-5 5ml
EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-50 50ml
EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 634

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 152.72

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

miRNA Universal SYBR qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 38.9

miRNA Universal SYBR qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 145

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 543.5

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 161

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 725

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns

QLMM10 each Ask for price

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 634

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns

QLMM17 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns

QLMM02 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns

QLMM07 each Ask for price

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 634

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL
EUR 328.5

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL
EUR 76.5

ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns

QLMM12-R each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns

QLMM17-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns

QLMM02-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns

QLMM07-R each Ask for price

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns

QLMM12-LR each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM17-LR each Ask for price

BrightCycle Universal SYBR Green qPCR Mix with UDG

RK21219 5mL
EUR 475

ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns

QLMM02-LR each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM07-LR each Ask for price

PCR Master mix (SYBR)

abx460031-1096tests 10 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-596tests 5 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-96tests 96 tests
EUR 337.5

Genious 2X SYBR Green Fast qPCR Mix (No ROX)

RK21205 1 ml
EUR 328.5

qPCR Master Mix

abx460014-1096tests 10 × 96 tests Ask for price

qPCR Master Mix

abx460014-596tests 5 × 96 tests Ask for price

qPCR Master Mix

abx460014-96tests 96 tests
EUR 337.5

1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen

Z-PPLUS-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)

RK21206 1 ml
EUR 328.5

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)

RK21207 1 ml
EUR 93

1ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-2ML 2ml
EUR 161
Description: Precision®FAST

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 1ml
EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 2x1ml
EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 5x1ml
EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 10x1ml
EUR 1632

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I, 150rxns

QLMM14 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II, 150rxns

QLMM18 each Ask for price

Maximo Dry-Master Mix (Lyophilized qPCR Mastermix with Sybrgreen)

S295RS 192 rcs (20µl, in plates or tubes)
EUR 171

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I, 150rxns

QLMM04 each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II, 150rxns

QLMM08 each Ask for price

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 349.2

1ml PrecisionPLUS qPCR Master Mix

Z-PPLUS-1ML 1ml
EUR 81
Description: Precision®PLUS

1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield)

W141 200 rxn
EUR 199

Apex qPCR Probe Master Mix

42-116P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR Probe Master Mix

42-117P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR Probe Master Mix

42-118P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Low ROX, 5ml

Apex qPCR Probe Master Mix

42-119P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR Probe Master Mix

42-120P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR Probe Master Mix

42-121P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, High ROX, 50ml

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 120.55

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 543.5

ViPrimePLUS Taq qPCR Master Mix, 150rxns

QLMM11 each Ask for price

HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
EUR 251.4

HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 115.68

ViPrimePLUS AtTaq qPCR Master Mix, 150rxns

QLMM01 each Ask for price

1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility)

W142 200 rxn
EUR 199

1ml Precision FAST qPCR Master Mix

Z-PFAST-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix

Z-PFAST-2ML 2ml
EUR 161
Description: Precision®FAST

PanGreen™ Universal SYBR® Green Mastermix

QSD01-0100 100 rxns
EUR 20

PanGreen™ Universal SYBR Green Mastermix UNG

QSD02-0100 100 rxns
EUR 22

1ml PrecisionPLUS CL qPCR Master Mix

Z-PPLUS-CL-1ML 1ml
EUR 81
Description: Precision®PLUS

1ml PrecisionPLUS LR qPCR Master Mix

Z-PPLUS-LR-1ML 1ml
EUR 81
Description: Precision®PLUS

ViPrimePLUS Lyophilized qPCR Master Mix, 150rxns

QLMM05 each Ask for price

Entrans 2X qPCR Probe Master Mix

RK21208 40 RXN
EUR 119

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns

QLMM14-R each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns

QLMM18-R each Ask for price

1ml Precision FAST CL qPCR Master Mix

Z-PFAST-CL-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST CL qPCR Master Mix

Z-PFAST-CL-2ML 2ml
EUR 161
Description: Precision®FAST

1ml Precision FAST LR qPCR Master Mix

Z-PFAST-LR-1ML 1ml
EUR 90
Description: Precision®FAST

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with ROX, 150rxns

QLMM04-R each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with ROX, 150rxns

QLMM08-R each Ask for price

Green-2-Go qPCR Mastermix (500X20ul Rxn)

QPCR004-S 4X1.25ml
EUR 303.25

1ml PrecisionPLUS CL & SY qPCR Master Mix

Z-PPLUS-CL-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

1ml PrecisionPLUS iC & SY qPCR Master Mix

Z-PPLUS-iC-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

1ml PrecisionPLUS LR & SY qPCR Master Mix

Z-PPLUS-LR-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

1ml PrecisionPLUS MX & SY qPCR Master Mix

Z-PPLUS-MX-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

1ml PrecisionPLUS R & SY qPCR Master Mix

Z-PPLUS-R-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

EZGreen 2x qPCR Master Mix, 40Rxns-50ul

A4157-1ML40Rxns 1ML (40Rxns)
EUR 55
Description: Biotechnology

1ml PrecisionPLUS qPCR Master Mix with ROX

Z-PPLUS-R-1ML 1ml
EUR 81
Description: Precision®PLUS

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns

QLMM14-LR each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns

QLMM18-LR each Ask for price

ABScript III RT Master Mix for qPCR

RK20428 10RXN
EUR 82.5

ABScript Neo RT Master Mix for qPCR

RK20432 100RXN
EUR 17.5

Entrans 2X qPCR Probe Master Mix V2

RK21212 50RXN
EUR 67.5

2 ml Precision FAST LR qPCR Master Mix

Z-PFAST-LR-2ML 2ml
EUR 161
Description: Precision®FAST

Compared, the identical inserts within the CpG-replete pCDNA3 vector demonstrated considerably decrease expression ranges and solely a minimal persistence of expression. β-galactosidase and enhanced inexperienced fluorescent protein genes inserted into pMBR2 vector additionally demonstrated increased expression ranges and higher upkeep in comparison with the identical genes in pCDNA3 vector.

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