Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3, and incRh4. Removing of those plasmids from their bacterial cells is a vital step in figuring out strain-specific virulence traits and to assemble strains helpful for transformation. Right here, we developed two highly effective instruments to enhance this course of. We first established a reporter system to detect the presence and absence of Ti/Ri plasmids in cells by utilizing an acetosyringone (AS)-inducible promoter of the Ti2 small RNA and luxAB from Vibrio harveyi. This technique distinguished a Ti/Ri plasmid-free cell colony amongst plasmid-harboring cell colonies by inflicting the latter colonies to emit gentle in response to AS.
We then constructed new “Ti/Ri eviction plasmids,” every of which carries a repABC from certainly one of 4 Ti/Ri plasmids that belonged to incRh1, incRh2, incRh3, and incRh4 teams within the suicidal plasmid pK18mobsacB and in a broad-host-range pBBR1 vector. Introduction of the brand new eviction plasmids into Agrobacterium cells harboring the corresponding Ti/Ri plasmids led to Ti/Ri plasmid-free cells in each incRh group. The Ti/Ri eviction was simpler by plasmids with the pBBR1 spine than by these with the pK18mobsacB spine. Moreover, the extremely steady cryptic plasmid pAtC58 in A. tumefaciens C58 was successfully evicted by the introduction of a pBBR1 vector containing the repABC of pAtC58. These outcomes point out that the set of pBBR1-repABC plasmids is a strong device for the removing of steady rhizobial plasmids.
Evaluation of UV spectrophotometry for dedication of plasmid DNA focus in vector preparations for human gene remedy merchandise.
The European Pharmacopoeia (Ph. Eur.) normal chapter 5.14. Gene switch medicinal merchandise for human use suggests the usage of absorbance measurements at 260 nm to find out the DNA focus of plasmid vectors used for the preparation of gene remedy merchandise for human use. A global collaborative examine was organised by the European Directorate for the High quality of Medicines & HealthCare (EDQM) to verify the suitability of UV spectrophotometry for the quantification of plasmid vectors utilized in gene remedy (GT).
Three Official Drugs Management Laboratories (OMCLs of the European OMCL Community) and members of the OMCL Working Group for GT merchandise took half within the examine, during which varied kinds of spectrophotometers have been assessed utilizing widespread take a look at samples. Outcomes of the examine demonstrated that UV spectrophotometry may be thought-about appropriate for the quantification of plasmid DNA in GT merchandise whatever the instrument used.
All formulations confirmed low vital micelle focus (CMC) values meaning extremely steady in serum containing medium. Polymeric micelles have been additionally evaluated for his or her stability within the presence of serum and nuclease in addition to cytotoxicity and transfection effectivity. All our outcomes proved that our novel polymeric micellar system ready by PPP60 block copolymer provide to be an environment friendly promising provider for gene supply functions. Furthermore, these findings contribute to design and improvement of novel gene vectors with tunable and performance options and in addition to cut back the cytotoxicity of PEI by partial hydrolysis of PEtOx an alternate synthesis methodology to provide linear PEI.

Plasmid-Templated Management of DNA-Cyclodextrin Nanoparticle Morphology by Molecular Vector Design for Efficient Gene Supply.
Engineering self-assembled superstructures by complexation of plasmid DNA (pDNA) and single-isomer nanometric measurement macromolecules (molecular nanoparticles) is a promising technique for gene supply. Notably, the performance and general structure of the vector may be exactly molded on the atomic stage by chemical tailoring, thereby enabling unprecedented alternatives for construction/self-assembling/pDNA supply relationship research. The experimental findings are in settlement with a shift from chelate to cross-linking interactions on going from primary-face- to secondary-face-linked CD dimers, the pDNA accomplice appearing as an energetic payload and as a template.
Past this notion, by judiciously preorganizing the purposeful parts in cyclodextrin (CD)-based molecular nanoparticles by covalent dimerization, right here we display that the morphology of the ensuing nanocomplexes (CDplexes) may be tuned, from spherical to ellipsoidal, rod-type, or worm-like nanoparticles, which makes it doable to achieve understanding of their shape-dependent transfection properties. Most curiously, the transfection effectivity in numerous cells was proven to be in a different way impacted by modifications of the CDplex morphology, which has led to the identification of an optimum prototype for tissue-selective DNA supply to the spleen in vivo.
Persistence of plasmid-mediated expression of transgenes in human mesenchymal stem cells relies upon totally on CpG ranges of each vector and transgene.
Gene remedy and cell modification for medical functions utilizing plasmid vectors are thought-about to be a protected and promising technique. One of many main issues with plasmid vector-based constructs is a speedy decline of transgene expression in cells in vitro and in vivo. An vital position of CpG motifs or bacterial vector spine in expression silencing has been recommended. The pMBR2 vector with CpG-free luciferase insert demonstrated the very best persistence of expression, whereas the wild-type luciferase insert containing 97 CpG motifs demonstrated decrease expression upkeep in the identical vector.
2x SYBR Green qPCR Master Mix (Low ROX) |
B21702 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21402 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
AceQ qPCR SYBR® Green Master Mix |
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ SYBR qPCR Master Mix |
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix |
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
ChamQ SYBR Color qPCR Master Mix |
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix |
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 128.8 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 580 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 290.4 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 897 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 224 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
31020-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
31020-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Green-2-Go qPCR Mastermix (500X20ul Rxn) |
QPCR004-S |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
31003-2 |
Biotium |
50x1ML |
EUR 3739.2 |
Description: Minimum order quantity: 1 unit of 50x1ML |
Fast EvaGreen Master Mix for qPCR(200 rxn) |
31003 |
Biotium |
2x1ML |
EUR 244.8 |
Description: Minimum order quantity: 1 unit of 2x1ML |
Fast EvaGreen Master Mix for qPCR (500 rxn) |
31003-1 |
Biotium |
5x1ML |
EUR 463.2 |
Description: Minimum order quantity: 1 unit of 5x1ML |
Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
31041-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
RT Master Mix for qPCR (gDNA digester plus) |
HY-K0511 |
MedChemExpress |
1 mL (100 rxns) |
EUR 378 |
Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
QPCR003-iC |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
31041-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
31041-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
QPCR006-NODYE |
Bio Basic |
4X1.25ml |
EUR 326.22 |
|
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
QPCR002-L |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17282-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17283-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17286-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17287-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
31020-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
QPCR005-NODYE |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
31014-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
31014-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
31015-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
31015-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
S190 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
S190L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
31003-T |
Biotium |
1ML |
EUR 147.6 |
Description: Minimum order quantity: 1 unit of 1ML |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
31014-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
31015-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
S200 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
S200L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
9-31045 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
31042-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
31042-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
31042-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
9-31046 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
31014 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
S150 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
S150L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
CRISPY100A-1 |
SBI |
200 rxn |
EUR 412 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
S160 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
S160L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
31015 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
S210L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
SYBR Green I |
B1747-25 |
Biovision |
each |
EUR 1396.8 |
SYBR Green I |
B1747-5 |
Biovision |
each |
EUR 464.4 |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374L |
MiTeGen |
10 x 1,25 ml |
EUR 818 |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374S |
MiTeGen |
2 x 1,25 ml |
EUR 205 |
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S175L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-376L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-376S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNG lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-377L |
MiTeGen |
10 x 1,25 ml |
EUR 982 |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-377S |
MiTeGen |
2 x 1,25 ml |
EUR 246 |
Description: qPCR SybrMaster UNG highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
S215L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
S220L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
S225L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
CytoTraceâ„¢ Ultra Green |
21800-1mg |
AAT Bioquest |
1 mg |
EUR 278 |
|
Description: CMFDA (5-chloromethylfluorescein diacetate) has been used as a prominent cell tracker in the research community for a long time. |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
S170L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
S185 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials |
S185L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
RA910A-1 |
SBI |
20 profiles |
EUR 1329.6 |
|
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-001 |
GenDepot |
1ml |
EUR 175.2 |
amfiSure qGreen Q-PCR Master Mix(2X), No Rox |
Q5600-002 |
GenDepot |
2x1ml |
EUR 248.4 |
Compared, the identical inserts within the CpG-replete pCDNA3 vector demonstrated considerably decrease expression ranges and solely a minimal persistence of expression. β-galactosidase and enhanced inexperienced fluorescent protein genes inserted into pMBR2 vector additionally demonstrated increased expression ranges and higher upkeep in comparison with the identical genes in pCDNA3 vector.