Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917

Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an vital goal for the event of therapeutic engineered micro organism, benefiting from the wealth of data of E. coli biology and ease of manipulation. Bacterial artificial biology tasks generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nonetheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or therapy.

EcN natively accommodates plasmids pMUT1 and pMUT2, which haven’t any identified operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable useful materials into the extracellular house.

We then show that the engineered plasmids have been maintained in micro organism because the engineered micro organism move by the mouse GI tract with out choice, and that the secretion system stays useful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the speedy improvement of therapeutic EcN micro organism.

Amplification of EBNA-1 by a single-plasmid vector-based gene amplification system in HEK293 cells as an environment friendly transient gene expression system

Our earlier work confirmed that there’s a limitation in the usage of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification programs in dhfr-non-deficient HEK293 cells, as endogenous dhfr might intervene with the amplification course of. Within the current examine, we efficiently generated Epstein-Barr virus nuclear antigen-1 (EBNA-1)-amplified HEK293 cells in a dhfr-non-deficient HEK293 cell background utilizing a single-plasmid vector-based gene amplification system with shRNA concentrating on the three’-UTR of endogenous dhfr. The introduction of this shRNA effectively downregulated the expression of endogenous dhfr within the HEK293 cells with out affecting exogenous dhfr expression.

The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this examine have been larger than these obtained from EBNA-1-amplified clones that have been generated utilizing the traditional amplification in our earlier examine. In step with earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (q_p)-enhancing impact, slightly than by bettering cell progress or transfection effectivity.

As well as, the N-glycan profiles within the Fc-fusion protein produced utilizing this transient gene expression (TGE) system weren’t affected by EBNA-1 amplification. These outcomes point out the potential utility of EBNA-1-amplified mammalian cells, developed utilizing a single-plasmid vector-based gene amplification system, for environment friendly protein manufacturing.

Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917

Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA

The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and subsequently are largely demanded within the biopharmaceutical manufacturing. Nonetheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing price. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this examine, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.

Plasmids DNA with totally different proportion of SC, open-circular, and linearized varieties have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.

Nonetheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This examine demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, at the least underneath our manufacturing course of.

Cloning in Plasmid Vectors: Blunt-Finish Cloning

This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “right” ligation merchandise when cloning blunt-ended goal fragments, the 2 parts of DNA within the ligation response should be current at an applicable ratio.

Genorise® Universal DNA Mini Kit 100
GR101076-100	Genorise Scientific	100	EUR 357

Genorise® Universal DNA Mini Kit 200
GR101077	Genorise Scientific	200	EUR 567

Genorise® Universal DNA Mini Kit 500
GR101077-500	Genorise Scientific	500	EUR 1272

Methylamp Universal Methylated DNA Kit
P-1011	EpiGentek	Ask for price Ask for price Ask for price	20 Modifications 10 Modifications 20 Modifications

Allprep DNA/RNA/miRNA Universal Kit
2148A	Unibiotest	50 Tests	EUR 500

Allprep DNA/RNA/miRNA Universal Kit
2148B	Unibiotest	4*50 Tests	EUR 1800

QPCR Kit DNA Universal f Mmix - EACH
MOL6608	Scientific Laboratory Supplies	EACH	EUR 1581.9

QPCR Kit DNA Universal m Mmix - EACH
MOL6612	Scientific Laboratory Supplies	EACH	EUR 1581.9

VAHTS Universal DNA Library Prep Kit for MGI
NDM607-01	Vazyme	24 rxn	EUR 879.6

VAHTS Universal DNA Library Prep Kit for MGI
NDM607-02	Vazyme	96 rxn	EUR 2563.2

Universal Solution for DNA isolation (GeDI)
E3760-01	EURx	25ml	EUR 30.52

Universal Solution for DNA isolation (GeDI)
E3760-02	EURx	100ml	EUR 95.92

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-01-24rxns	Vazyme	24 rxns	EUR 304.66

VAHTS Universal DNA library Prep Kit for MGI V3
NDM607-02-96rxns	Vazyme	96 rxns	EUR 1139.05

VAHTS Universal DNA library Prep Kit for MGI V4
NDM610-01	Vazyme	24 rxns	EUR 235.2

VAHTS Universal DNA library Prep Kit for MGI V4
NDM610-02	Vazyme	96 rxns	EUR 880

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit
E3597-01	EURx	25preps	EUR 81.75
Description: Kit for isolation of total DNA/RNA/Protein from the same biological sample(bacteria, tissue, plant, yeast, cell culture)

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit
E3597-02	EURx	100preps	EUR 299.75
Description: Kit for isolation of total DNA/RNA/Protein from the same biological sample(bacteria, tissue, plant, yeast, cell culture)

Allprep RNA/DNA/miRNA/protein Universal Kit
2149A	Unibiotest	50 Tests	EUR 600

Allprep RNA/DNA/miRNA/protein Universal Kit
2149B	Unibiotest	4*50 Tests	EUR 2200

Methylamp Universal Methylated DNA Preparation Kit
P-1019	EpiGentek	40 µg	EUR 416.7

VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-01	Vazyme	24 rxn	EUR 529.2

VAHTS Universal DNA Library Prep Kit for Illumina V3
ND607-02	Vazyme	96 rxn	EUR 1686

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-01	Vazyme	24 rxns	EUR 294

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-01-24rxns	Vazyme	24 rxns	EUR 304.44

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-02	Vazyme	96 rxns	EUR 1100

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-02-96rxns	Vazyme	96 rxns	EUR 1139.05

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-03-24rxns	Vazyme	24 rxns	EUR 269.23

VAHTS Universal DNA Library Prep Kit for Illumina V4
ND610-04-96rxns	Vazyme	96 rxns	EUR 999.53

VAHTS Universal DNA Library Prep Kit for Ion Torrent
ND701-01	Vazyme	24 rxn	EUR 879.6

VAHTS Universal DNA Library Prep Kit for Ion Torrent
ND701-02	Vazyme	96 rxn	EUR 2563.2

AFTSpin Universal Plant Fast DNA Extraction Kit
RK30111	Abclonal	50RXN	EUR 68.13

Universal Genomic DNA Purification kit（Spin Column)
2546A	Unibiotest	50 Tests	EUR 100

Universal Genomic DNA Purification kit（Spin Column)
2546B	Unibiotest	100 Tests	EUR 150

EpiMag High Throughput DNA Isolation Universal Kit
P-1022	EpiGentek	96 Samples	EUR 208

EpiMag High Throughput DNA Isolation Universal Kit
P-1022-96	EpiGentek	96 Samples	EUR 227.7

Universal-1
OD308-1PK	EWC Diagnostics	1 unit	EUR 2.65
Description: Universal-1

Universal-1
OD308R-1PK	EWC Diagnostics	1 unit	EUR 2.65
Description: Universal-1

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2
ND702-01	Vazyme	24 rxns	EUR 294

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2
ND702-02	Vazyme	96 rxns	EUR 1100

Universal Kit for DNA isolation with GeDI Solution
E3765-01	EURx	25preps	EUR 59.95

Universal Kit for DNA isolation with GeDI Solution
E3765-02	EURx	100preps	EUR 210.37

VAHTS Universal Pro DNA Library Prep Kit for MGI
NDM608-01	Vazyme	24 rxn	EUR 907.2

VAHTS Universal Pro DNA Library Prep Kit for MGI
NDM608-02	Vazyme	96 rxn	EUR 2740.8

VAHTS Universal Plus DNA Library Prep Kit for MGI
NDM617-01	Vazyme	24 rxn	EUR 988.8

VAHTS Universal Plus DNA Library Prep Kit for MGI
NDM617-02	Vazyme	96 rxn	EUR 2889.6

VAHTS Universal Pro DNA Library Prep Kit for Illumina
ND608-01	Vazyme	24 rxn	EUR 558

VAHTS Universal Pro DNA Library Prep Kit for Illumina
ND608-02	Vazyme	96 rxn	EUR 1858.8

VAHTS Universal Plus DNA Library Prep Kit for MGI V2
NDM627-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal Plus DNA Library Prep Kit for MGI V2
NDM627-01	Vazyme	24 rxns	EUR 312.5

VAHTS Universal Plus DNA Library Prep Kit for MGI V2
NDM627-01-24rxns	Vazyme	24 rxns	EUR 323.59

VAHTS Universal Plus DNA Library Prep Kit for MGI V2
NDM627-02	Vazyme	96 rxns	EUR 1150

VAHTS Universal Plus DNA Library Prep Kit for MGI V2
NDM627-02-96rxns	Vazyme	96 rxns	EUR 1190.83

VAHTS Universal Plus DNA Library Prep Kit for Illumina
ND617-01	Vazyme	24 rxn	EUR 590.4

VAHTS Universal Plus DNA Library Prep Kit for Illumina
ND617-02	Vazyme	96 rxn	EUR 1926

Universal Holder
LBI10201	Westburg	each	EUR 161.87

Hexa Universal-2
HX038-1PK	EWC Diagnostics	1 unit	EUR 2.51
Description: Hexa Universal-2

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)
ND607-03	Vazyme	24 rxn	EUR 514.8

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)
ND607-04	Vazyme	96 rxn	EUR 1624.8

Universal Platform
SS-300-U	Avans Biotechnology Corp	each	EUR 250

Universal Indicator
I013-100ML	EWC Diagnostics	1 unit	EUR 2.65
Description: Universal Indicator

Universal Indicator
I013-500ML	EWC Diagnostics	1 unit	EUR 9.01
Description: Universal Indicator

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2
ND627-00-6rxns	Vazyme	6 rxns	EUR 1.09

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2
ND627-01	Vazyme	24 rxns	EUR 312.5

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2
ND627-01-24rxns	Vazyme	24 rxns	EUR 323.59

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2
ND627-02	Vazyme	96 rxns	EUR 1150

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2
ND627-02-96rxns	Vazyme	96 rxns	EUR 1190.83

Hexa Universal - 1
HX032-1PK	EWC Diagnostics	1 unit	EUR 2.51
Description: Hexa Universal - 1

Hexa Universal - 4
HX715-1PK	EWC Diagnostics	1 unit	EUR 2.51
Description: Hexa Universal - 4

Hexa Universal - 5
HX716-1PK	EWC Diagnostics	1 unit	EUR 2.51
Description: Hexa Universal - 5

Icosa Universal - 1
IC001-1PK	EWC Diagnostics	1 unit	EUR 9.32
Description: Icosa Universal - 1

Icosa Universal - 2
IC006-1PK	EWC Diagnostics	1 unit	EUR 9.32
Description: Icosa Universal - 2

Dodeca Universal -XI
DE026-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal -XI

Dodeca - Universal XV,
DE042-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca - Universal XV,

Universal V1-Puck
M-CP-111-021	MiTeGen	1 PUCK	EUR 350
Description: Universal V1-Puck

UNIVERSAL BEER AGAR
U21-100-10kg	Alphabiosciences	10 kg	EUR 1828.8

UNIVERSAL BEER AGAR
U21-100-2kg	Alphabiosciences	2kg	EUR 444

UNIVERSAL BEER AGAR
U21-100-500g	Alphabiosciences	500 g	EUR 164.4

Dodeca Universal-I
DE001-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-I

Dodeca Universal-V
DE013-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-V

Dodeca Universal -XII
DE027-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal -XII

Dodeca Universal XIV
DE035-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal XIV

Universal WB Toolkit
KTD106-EN-20T	Abbkine	20 T	EUR 59
Description: Abbkine has developed a Universal WB Toolkit. After careful design and research, one-stop operation can meet the needs of routine WB experiments and obtain excellent experimental results.

Universal WB Toolkit
KTD106-EN-each	Abbkine	each	Ask for price

Universal WB Toolkit
KTD106-EN-Null	Abbkine	Null	Ask for price

Universal WB Toolkit
MBS9719335-20Tests	MyBiosource	20Tests	EUR 195

Universal WB Toolkit
MBS9719335-5x20Tests	MyBiosource	5x20Tests	EUR 885

Dodeca Universal-II
DE007-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-II

Dodeca Universal-IV
DE012-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-IV

Dodeca Universal-VI
DE014-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-VI

Dodeca Universal-IX
DE017-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-IX

Dodeca Universal- XIII
DE028-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal- XIII

Dodeca Universal-III
DE008-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-III

Dodeca Universal-VII
DE015-1PK	EWC Diagnostics	1 unit	EUR 3.96
Description: Dodeca Universal-VII

Universal Basal Medium
cAP-01C	Angio Proteomie	500ml	EUR 64.35

APP Antibody (universal)
GWB-C298C6	GenWay Biotech	0.1 ml	Ask for price

Universal Block Buffer
TBS5013	Tribioscience	100 ml	EUR 60

NBS Universal Platform
SHA4070	Scientific Laboratory Supplies	EACH	EUR 708.93

NBS Universal Platform
SHA4232	Scientific Laboratory Supplies	EACH	EUR 388.8

NBS Universal Platform
SHA4256	Scientific Laboratory Supplies	EACH	EUR 476.61

NBS Universal Platform
SHA4272	Scientific Laboratory Supplies	EACH	EUR 756.04

NBS Universal Platform
SHA4290	Scientific Laboratory Supplies	EACH	EUR 1016.3

Universal qPCR Mixture
QPCR1000-UNIV	Bio Basic	4X1.25ml	EUR 331.44

Universal Growth Medium
cAP-01B	Angio Proteomie	500ml	EUR 89.1

Universal platform - EACH
INC1112	Scientific Laboratory Supplies	EACH	EUR 1613.25

Universal vessel holder
LBI10230	Westburg	each	EUR 156.96

*Universal Liquid Medium
M1332-500G	EWC Diagnostics	1 unit	EUR 25.78
Description: *Universal Liquid Medium

Universal Heating System
LBI10501	Westburg	each	EUR 11281.5

100-1250µL Universal Fit
2370	Biotix	1,000/PACK, 4/CASE	EUR 120.08

100-1250µL Universal Fit
2370.N	Biotix	1,000/PACK, 4/CASE	EUR 45.25

100-1250µL Universal Fit
2372	Biotix	8/PACK, 4/CASE	EUR 108.48

100-1250µL Universal Fit
2372.N	Biotix	8/PACK, 4/CASE	EUR 103.84

100-1250µL Universal Fit
2372.NS	Biotix	8/PACK, 4/CASE	EUR 108.48

100-1250µL Universal Fit
2372.S	Biotix	8/PACK, 4/CASE	EUR 115.44

100-1250µL Universal Fit
2377	Biotix	10/PACK, 5/CASE	EUR 120.08

100-1250µL Universal Fit
2377.N	Biotix	10/PACK, 5/CASE	EUR 116.02

20-300µL Universal Fit
2090.N	Biotix	1,000/PACK, 10/CASE	EUR 89.92

20-300µL Universal Fit
2092	Biotix	10/PACK, 5/CASE	EUR 127.04

20-300µL Universal Fit
2092.N	Biotix	10/PACK, 5/CASE	EUR 122.98

20-300µL Universal Fit
2092.NS	Biotix	8/PACK, 4/CASE	EUR 127.04

20-300µL Universal Fit
2092.S	Biotix	10/PACK, 5/CASE	EUR 134.58

20-300µL Universal Fit
2097.N	Biotix	10/PACK, 10/CASE	EUR 174.03

20-280µL Universal Fit
2152.96.N	Biotix	8/PACK, 4/CASE	EUR 519.77

10-280µL Universal Fit
2152.96.NS	Biotix	8/PACK, 4/CASE	EUR 555.74

100-1000µL Universal Fit
2160	Biotix	1,000/PACK, 4/CASE	EUR 45.25

100-1000µL Universal Fit
2162.96	Biotix	8/PACK, 4/CASE	EUR 130.52

100-1000µL Universal Fit
2162.96.S	Biotix	8/PACK, 4/CASE	EUR 108.48

100-1000µL Universal Fit
2167.96	Biotix	10/PACK, 5/CASE	EUR 92.82

0.1-10µL Universal Fit
2047	Biotix	20/PACK, 4/CASE	EUR 137.48

0.1-10µL Universal Fit
2047.N	Biotix	20/PACK, 4/CASE	EUR 138.64

20-200µL Universal Fit
2100	Biotix	1,000/PACK, 10/CASE	EUR 105

20-200µL Universal Fit
2100.N	Biotix	1,000/PACK, 10/CASE	EUR 89.92

20-200µL Universal Fit
2102.N	Biotix	10/PACK, 5/CASE	EUR 122.98

1-20µL Universal Fit
2102.NS	Biotix	10/PACK, 5/CASE	EUR 127.04

20-200µL Universal Fit
2102.S	Biotix	10/PACK, 5/CASE	EUR 134.58

20-200µL Universal Fit
2107.N	Biotix	10/PACK, 10/CASE	EUR 174.03

0.1-10µL Universal Fit
2040	Biotix	1,000/PACK, 20/CASE	EUR 240.16

0.1-10µL Universal Fit
2040.N	Biotix	1,000/PACK, 20/CASE	EUR 255.24

0.1-10µL Universal Fit
2042.N	Biotix	10/PACK, 5/CASE	EUR 122.98

0.1-10µL Universal Fit
2042.NS	Biotix	10/PACK, 5/CASE	EUR 127.04

0.1-10µL Universal Fit
2042.S	Biotix	10/PACK, 5/CASE	EUR 134.58

0.1-10µL Universal Fit
BT10	Biotix	10 trays of 96 / pack	EUR 231.46
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

0.1-10µL Universal Fit
BT10.N	Biotix	10/PACK, 5/CASE	EUR 224.5

10-100µL Universal Fit
BT100	Biotix	10 trays of 96 / pack	EUR 231.46
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

100-1000µL Universal Fit
BT1000.96	Biotix	8 trays of 96 / pack	EUR 203.62
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

100-1000µL Universal Fit
BT1000.96.N	Biotix	8/PACK, 4/CASE	EUR 195.49

100-1250µL Universal Fit
BT1250	Biotix	8 trays of 96 / pack	EUR 209.42
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

100-1250µL Universal Fit
BT1250.N	Biotix	8/PACK, 4/CASE	EUR 202.46

1-20µL Universal Fit
BT20	Biotix	10 trays of 96 / pack	EUR 231.46
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

20-200µL Universal Fit
BT200	Biotix	10 trays of 96 / pack	EUR 231.46
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

10-230µL Universal Fit
BT200XLT	Biotix	8/PACK, 4/CASE	EUR 718.16

20-300µL Universal Fit
BT300	Biotix	10 trays of 96 / pack	EUR 231.46
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Universal Spring Platform
H1000-P-SP	Benchmark Scientific	1 PC	EUR 660.13

Universal Spring Platform
H1010-P-SP	Benchmark Scientific	1 each	EUR 697.83

Universal Attachment - EACH
MIX1320	Scientific Laboratory Supplies	EACH	EUR 322.65

Universal Coating Solution
MBS556488-20mL	MyBiosource	20mL	EUR 310

Universal Coating Solution
MBS556488-5x20mL	MyBiosource	5x20mL	EUR 1235

Universal Oven UN30 - EACH
OVE8314	Scientific Laboratory Supplies	EACH	EUR 1881.9

Universal Oven UN55 - EACH
OVE8316	Scientific Laboratory Supplies	EACH	EUR 2196.45

Universal Oven UN75 - EACH
OVE8318	Scientific Laboratory Supplies	EACH	EUR 2281.5

Universal Oven UF30 - EACH
OVE8332	Scientific Laboratory Supplies	EACH	EUR 2150.55

Universal Oven UF55 - EACH
OVE8334	Scientific Laboratory Supplies	EACH	EUR 2594.7

Universal Oven UF75 - EACH
OVE8336	Scientific Laboratory Supplies	EACH	EUR 2737.8

Universal Restriction Buffer
EN-300	Jena Bioscience GmbH	5 x 1ml	EUR 17.16

Grant Universal Tray - EACH
BAT4622	Scientific Laboratory Supplies	EACH	EUR 353.7

Universal Oven UN110 - EACH
OVE8320	Scientific Laboratory Supplies	EACH	EUR 2922.75

Universal Oven UN160 - EACH
OVE8322	Scientific Laboratory Supplies	EACH	EUR 3524.85

Universal Oven UN260 - EACH
OVE8324	Scientific Laboratory Supplies	EACH	EUR 4666.95

Universal Oven UN450 - EACH
OVE8326	Scientific Laboratory Supplies	EACH	EUR 6176.25

Universal Oven UN750 - EACH
OVE8328	Scientific Laboratory Supplies	EACH	EUR 8275.5

Universal Oven UF110 - EACH
OVE8338	Scientific Laboratory Supplies	EACH	EUR 3235.95

Universal Oven UF160 - EACH
OVE8340	Scientific Laboratory Supplies	EACH	EUR 4207.95

Universal Oven UF260 - EACH
OVE8342	Scientific Laboratory Supplies	EACH	EUR 5364.9

Universal Oven UF450 - EACH
OVE8344	Scientific Laboratory Supplies	EACH	EUR 7025.4

Universal Oven UF750 - EACH
OVE8346	Scientific Laboratory Supplies	EACH	EUR 9309.6

If the molar ratio of plasmid vector to focus on DNA is simply too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the international DNA and the variety of inserts in every recombinant clone should all the time be validated by restriction endonuclease mapping or another means.

Amplification of EBNA-1 by a single-plasmid vector-based gene amplification system in HEK293 cells as an environment friendly transient gene expression system

Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA

Cloning in Plasmid Vectors: Blunt-Finish Cloning

Genorise® Universal DNA Mini Kit 100

Genorise® Universal DNA Mini Kit 200

Genorise® Universal DNA Mini Kit 500

Methylamp Universal Methylated DNA Kit

Allprep DNA/RNA/miRNA Universal Kit

Allprep DNA/RNA/miRNA Universal Kit

QPCR Kit DNA Universal f Mmix - EACH

QPCR Kit DNA Universal m Mmix - EACH

VAHTS Universal DNA Library Prep Kit for MGI

VAHTS Universal DNA Library Prep Kit for MGI

Universal Solution for DNA isolation (GeDI)

Universal Solution for DNA isolation (GeDI)

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V3

VAHTS Universal DNA library Prep Kit for MGI V4

VAHTS Universal DNA library Prep Kit for MGI V4

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit

GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit

Allprep RNA/DNA/miRNA/protein Universal Kit

Allprep RNA/DNA/miRNA/protein Universal Kit

Methylamp Universal Methylated DNA Preparation Kit

VAHTS Universal DNA Library Prep Kit for Illumina V3

VAHTS Universal DNA Library Prep Kit for Illumina V3

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Illumina V4

VAHTS Universal DNA Library Prep Kit for Ion Torrent

VAHTS Universal DNA Library Prep Kit for Ion Torrent

AFTSpin Universal Plant Fast DNA Extraction Kit

Universal Genomic DNA Purification kit（Spin Column)

Universal Genomic DNA Purification kit（Spin Column)

EpiMag High Throughput DNA Isolation Universal Kit

EpiMag High Throughput DNA Isolation Universal Kit

Universal-1

Universal-1

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

Universal Kit for DNA isolation with GeDI Solution

Universal Kit for DNA isolation with GeDI Solution

VAHTS Universal Pro DNA Library Prep Kit for MGI

VAHTS Universal Pro DNA Library Prep Kit for MGI

VAHTS Universal Plus DNA Library Prep Kit for MGI

VAHTS Universal Plus DNA Library Prep Kit for MGI

VAHTS Universal Pro DNA Library Prep Kit for Illumina

VAHTS Universal Pro DNA Library Prep Kit for Illumina

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina

VAHTS Universal Plus DNA Library Prep Kit for Illumina

Universal Holder

Hexa Universal-2

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

Universal Platform

Universal Indicator

Universal Indicator

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

Hexa Universal - 1

Hexa Universal - 4

Hexa Universal - 5

Icosa Universal - 1

Icosa Universal - 2

Dodeca Universal -XI

Dodeca - Universal XV,