Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an vital goal for the event of therapeutic engineered micro organism, benefiting from the wealth of data of E. coli biology and ease of manipulation. Bacterial artificial biology tasks generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nonetheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or therapy.
EcN natively accommodates plasmids pMUT1 and pMUT2, which haven’t any identified operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable useful materials into the extracellular house.
We then show that the engineered plasmids have been maintained in micro organism because the engineered micro organism move by the mouse GI tract with out choice, and that the secretion system stays useful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the speedy improvement of therapeutic EcN micro organism.
Amplification of EBNA-1 by a single-plasmid vector-based gene amplification system in HEK293 cells as an environment friendly transient gene expression system
Our earlier work confirmed that there’s a limitation in the usage of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification programs in dhfr-non-deficient HEK293 cells, as endogenous dhfr might intervene with the amplification course of. Within the current examine, we efficiently generated Epstein-Barr virus nuclear antigen-1 (EBNA-1)-amplified HEK293 cells in a dhfr-non-deficient HEK293 cell background utilizing a single-plasmid vector-based gene amplification system with shRNA concentrating on the three’-UTR of endogenous dhfr. The introduction of this shRNA effectively downregulated the expression of endogenous dhfr within the HEK293 cells with out affecting exogenous dhfr expression.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this examine have been larger than these obtained from EBNA-1-amplified clones that have been generated utilizing the traditional amplification in our earlier examine. In step with earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell progress or transfection effectivity.
As well as, the N-glycan profiles within the Fc-fusion protein produced utilizing this transient gene expression (TGE) system weren’t affected by EBNA-1 amplification. These outcomes point out the potential utility of EBNA-1-amplified mammalian cells, developed utilizing a single-plasmid vector-based gene amplification system, for environment friendly protein manufacturing.
Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA
The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and subsequently are largely demanded within the biopharmaceutical manufacturing. Nonetheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing price. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this examine, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.
Plasmids DNA with totally different proportion of SC, open-circular, and linearized varieties have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.
Nonetheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This examine demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, at the least underneath our manufacturing course of.
Cloning in Plasmid Vectors: Blunt-Finish Cloning
This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “right” ligation merchandise when cloning blunt-ended goal fragments, the 2 parts of DNA within the ligation response should be current at an applicable ratio.
100 BP DNA LADDER, 500UL PER KIT |
|||
| M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
|
Description: Bioscience Mol Bio; DNA Ladder |
|||
1 KB DNA LADDER, 500UL PER KIT |
|||
| M-DNA-1KB | CORNING | 1/pk | EUR 84 |
|
Description: Bioscience Mol Bio; DNA Ladder |
|||
QPCR Kit DNA Universal f Mmix |
|||
| MOL6608 | Scientific Laboratory Supplies | EACH | EUR 1127.46 |
QPCR Kit DNA Universal m Mmix |
|||
| MOL6612 | Scientific Laboratory Supplies | EACH | EUR 1127.46 |
Methylamp Universal Methylated DNA Preparation Kit |
|||
| P-1019 | EpiGentek | 40 µg | EUR 416.7 |
Genorise® Universal DNA Mini Kit 50 |
|||
| GR101076 | Genorise Scientific | 50 | EUR 267.6 |
Genorise® Universal DNA Mini Kit 100 |
|||
| GR101076-100 | Genorise Scientific | 100 | EUR 428.4 |
Genorise® Universal DNA Mini Kit 200 |
|||
| GR101077 | Genorise Scientific | 200 | EUR 680.4 |
Genorise® Universal DNA Mini Kit 500 |
|||
| GR101077-500 | Genorise Scientific | 500 | EUR 1526.4 |
EpiMag High Throughput DNA Isolation Universal Kit |
|||
| P-1022-96 | EpiGentek | 96 Samples | EUR 227.7 |
2K DNA Marker |
|||
| 20-abx098045 | Abbexa |
|
|
5K DNA Marker |
|||
| 20-abx098048 | Abbexa |
|
|
15K DNA Marker |
|||
| 20-abx098049 | Abbexa |
|
|
1Kb DNA Marker |
|||
| 20-abx098050 | Abbexa |
|
|
100bp DNA Marker |
|||
| 20-abx098052 | Abbexa |
|
|
100bp DNA Marker |
|||
| D1100-050 | GenDepot | 50㎍ | EUR 198 |
100bp DNA Marker |
|||
| D1100-250 | GenDepot | 5X50㎍ | EUR 660 |
1Kb DNA Marker |
|||
| D1101-050 | GenDepot | 50㎍ | EUR 157.2 |
1Kb DNA Marker |
|||
| D1101-250 | GenDepot | 5X50㎍ | EUR 360 |
DL5000 DNA Marker |
|||
| MD102-01 | Vazyme | 250 μl | EUR 128.4 |
DL5000 DNA Marker |
|||
| MD102-02 | Vazyme | 500 μl | EUR 135.6 |
DL15000 DNA Marker |
|||
| MD103-01 | Vazyme | 250 μl | EUR 130.8 |
DL15000 DNA Marker |
|||
| MD103-02 | Vazyme | 500 μl | EUR 138 |
VAHTS Universal DNA Library Prep Kit for MGI |
|||
| NDM607-01 | Vazyme | 24 rxn | EUR 879.6 |
VAHTS Universal DNA Library Prep Kit for MGI |
|||
| NDM607-02 | Vazyme | 96 rxn | EUR 2563.2 |
1Kb Plus DNA Marker |
|||
| 20-abx098051 | Abbexa |
|
|
DL2000 Plus DNA Marker |
|||
| MD101-01 | Vazyme | 250 μl | EUR 128.4 |
DL2000 Plus DNA Marker |
|||
| MD101-02 | Vazyme | 500 μl | EUR 135.6 |
Linear Kinetoplast DNA Marker |
|||
| TG2018-1 | TopoGen | 10 ug | EUR 214.8 |
Decatenated Kinetoplast DNA Marker |
|||
| TG2020-1 | TopoGen | 10 ug | EUR 228 |
Relaxed pRYG DNA Marker |
|||
| TG2025-1 | TopoGen | 10 ug | EUR 321.6 |
Linear pRYG DNA Marker |
|||
| TG2028-1 | TopoGen | 10 ug | EUR 214.8 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 |
|||
| ND607-01 | Vazyme | 24 rxn | EUR 529.2 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 |
|||
| ND607-02 | Vazyme | 96 rxn | EUR 1686 |
VAHTS Universal Pro DNA Library Prep Kit for Illumina |
|||
| ND608-01 | Vazyme | 24 rxn | EUR 558 |
VAHTS Universal Pro DNA Library Prep Kit for Illumina |
|||
| ND608-02 | Vazyme | 96 rxn | EUR 1858.8 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina |
|||
| ND617-01 | Vazyme | 24 rxn | EUR 590.4 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina |
|||
| ND617-02 | Vazyme | 96 rxn | EUR 1926 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent |
|||
| ND701-01 | Vazyme | 24 rxn | EUR 879.6 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent |
|||
| ND701-02 | Vazyme | 96 rxn | EUR 2563.2 |
VAHTS Universal Pro DNA Library Prep Kit for MGI |
|||
| NDM608-01 | Vazyme | 24 rxn | EUR 907.2 |
VAHTS Universal Pro DNA Library Prep Kit for MGI |
|||
| NDM608-02 | Vazyme | 96 rxn | EUR 2740.8 |
VAHTS Universal Plus DNA Library Prep Kit for MGI |
|||
| NDM617-01 | Vazyme | 24 rxn | EUR 988.8 |
VAHTS Universal Plus DNA Library Prep Kit for MGI |
|||
| NDM617-02 | Vazyme | 96 rxn | EUR 2889.6 |
1 Kb Ladder DNA Marker |
|||
| FYD001-500UL | Yeastern Biotech | 20 µg/ 500 µl | Ask for price |
100 bp Ladder DNA Marker |
|||
| FYD002-500UL | Yeastern Biotech | 30 µg/ 500 µl | Ask for price |
50 bp Ladder DNA Marker |
|||
| FYD004-500UL | Yeastern Biotech | 32.5 µg/ 500 µl | Ask for price |
Universal platform |
|||
| INC1112 | Scientific Laboratory Supplies | EACH | EUR 1265.4 |
Universal Attachment |
|||
| MIX1320 | Scientific Laboratory Supplies | EACH | EUR 272.46 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
|||
| ND607-03 | Vazyme | 24 rxn | EUR 514.8 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
|||
| ND607-04 | Vazyme | 96 rxn | EUR 1624.8 |
500-10000bp DNA Marker, Original Form |
|||
| M101O-1 | Bio Basic | 100loading, 100prep | EUR 110.11 |
500-10000bp DNA Marker, Original Form |
|||
| M101O-2 | Bio Basic | 5x100loading, 500prep | EUR 263.58 |
100-1500bp DNA Marker, Original Form |
|||
| M102O-1 | Bio Basic | 100loading, 100prep | EUR 108.02 |
100-1500bp DNA Marker, Original Form |
|||
| M102O-2 | Bio Basic | 5x100loading, 500prep | EUR 258.36 |
100-1500bp DNA Marker, Original Form |
|||
| M107O-1 | Bio Basic | 100loading, 100prep | EUR 110.11 |
100-1500bp DNA Marker, Original Form |
|||
| M107O-2 | Bio Basic | 5x100loading, 500prep | EUR 263.58 |
Image Green? 100 bp DNA Marker |
|||
| M1155-500 | Biovision | EUR 352.8 | |
Image Green? 1 kb DNA Marker |
|||
| M1156-500 | Biovision | EUR 352.8 | |
50-500bp Low Range DNA Marker |
|||
| GM305 | Bio Basic | 200loading, 200prep | EUR 137.78 |
2K Plus DNA Marker (8 linear double-stranded DNA bands) |
|||
| 20-abx098046 | Abbexa |
|
|
2K Plus DNA Marker (9 linear double-stranded DNA bands) |
|||
| 20-abx098047 | Abbexa |
|
|
100bp Plus DNA Marker (12 linear double-stranded DNA bands) |
|||
| 20-abx098053 | Abbexa |
|
|
100bp Plus DNA Marker (14 linear double-stranded DNA bands) |
|||
| 20-abx098054 | Abbexa |
|
|
Universal Detachment Solution |
|||
| AD002 | Neuromics | 100 ml | EUR 177.6 |
Universal Neutralization Buffer |
|||
| NB001 | Neuromics | 100 ml | EUR 162 |
Universal Spring Platform |
|||
| H1000-P-SP | Benchmark Scientific | 1 PC | EUR 388.07 |
Universal qPCR Mixture |
|||
| QPCR1000-UNIV | Bio Basic | 4X1.25ml | EUR 331.44 |
UNIVERSAL BEER AGAR |
|||
| U21-100-10kg | Alphabiosciences | 10 kg | EUR 1828.8 |
UNIVERSAL BEER AGAR |
|||
| U21-100-2kg | Alphabiosciences | 2kg | EUR 444 |
UNIVERSAL BEER AGAR |
|||
| U21-100-500g | Alphabiosciences | 500 g | EUR 164.4 |
UNIVERSAL PREENRICHMENT BROTH |
|||
| U21-101-10kg | Alphabiosciences | 10 kg | EUR 898.8 |
UNIVERSAL PREENRICHMENT BROTH |
|||
| U21-101-2Kg | Alphabiosciences | 2 Kg | EUR 242.4 |
UNIVERSAL PREENRICHMENT BROTH |
|||
| U21-101-500g | Alphabiosciences | 500 g | EUR 109.2 |
Mains adaptor (universal) |
|||
| ARG1248 | Scientific Laboratory Supplies | EACH | EUR 118.56 |
Grant Universal Tray |
|||
| BAT4622 | Scientific Laboratory Supplies | EACH | EUR 275.88 |
Universal Spring Tray |
|||
| BAT7177 | Scientific Laboratory Supplies | EACH | EUR 259.92 |
Universal Stratus Adapter |
|||
| MIC6036 | Scientific Laboratory Supplies | EACH | EUR 445.74 |
Loopster Universal Disc |
|||
| MIX1080 | Scientific Laboratory Supplies | EACH | EUR 171 |
Universal (Aluminum) Platform |
|||
| MIX1322 | Scientific Laboratory Supplies | EACH | EUR 132.24 |
Universal Spring Platform |
|||
| MIX7184 | Scientific Laboratory Supplies | EACH | EUR 738.72 |
Universal attachment IKA |
|||
| SHA2210 | Scientific Laboratory Supplies | EACH | EUR 744.42 |
NBS Universal Platform |
|||
| SHA4050 | Scientific Laboratory Supplies | EACH | EUR 1288.2 |
NBS Universal Platform |
|||
| SHA4070 | Scientific Laboratory Supplies | EACH | EUR 934.8 |
NBS Universal Platform |
|||
| SHA4232 | Scientific Laboratory Supplies | EACH | EUR 513 |
NBS Universal Platform |
|||
| SHA4256 | Scientific Laboratory Supplies | EACH | EUR 628.14 |
NBS Universal Platform |
|||
| SHA4272 | Scientific Laboratory Supplies | EACH | EUR 996.36 |
NBS Universal Platform |
|||
| SHA4290 | Scientific Laboratory Supplies | EACH | EUR 1340.64 |
Universal AC adapter |
|||
| PIP3214 | Scientific Laboratory Supplies | EACH | EUR 62.93 |
Universal Multichannel Stand |
|||
| F161417 | Scientific Laboratory Supplies | EACH | EUR 28.5 |
Universal Oven UN30 |
|||
| OVE8314 | Scientific Laboratory Supplies | EACH | EUR 1381.68 |
Universal Oven UN55 |
|||
| OVE8316 | Scientific Laboratory Supplies | EACH | EUR 1648.44 |
Universal Oven UN75 |
|||
| OVE8318 | Scientific Laboratory Supplies | EACH | EUR 1714.56 |
Universal Oven UN110 |
|||
| OVE8320 | Scientific Laboratory Supplies | EACH | EUR 2194.5 |
Universal Oven UN160 |
|||
| OVE8322 | Scientific Laboratory Supplies | EACH | EUR 2648.22 |
Universal Oven UN260 |
|||
| OVE8324 | Scientific Laboratory Supplies | EACH | EUR 3505.5 |
Universal Oven UN450 |
|||
| OVE8326 | Scientific Laboratory Supplies | EACH | EUR 4639.8 |
Universal Oven UN750 |
|||
| OVE8328 | Scientific Laboratory Supplies | EACH | EUR 6305.34 |
Universal Oven UF30 |
|||
| OVE8332 | Scientific Laboratory Supplies | EACH | EUR 1614.24 |
Universal Oven UF55 |
|||
| OVE8334 | Scientific Laboratory Supplies | EACH | EUR 1948.26 |
Universal Oven UF75 |
|||
| OVE8336 | Scientific Laboratory Supplies | EACH | EUR 2056.56 |
Universal Oven UF110 |
|||
| OVE8338 | Scientific Laboratory Supplies | EACH | EUR 2401.98 |
Universal Oven UF160 |
|||
| OVE8340 | Scientific Laboratory Supplies | EACH | EUR 3161.22 |
Universal Oven UF260 |
|||
| OVE8342 | Scientific Laboratory Supplies | EACH | EUR 4031.04 |
Universal Oven UF450 |
|||
| OVE8344 | Scientific Laboratory Supplies | EACH | EUR 5278.2 |
Universal Oven UF750 |
|||
| OVE8346 | Scientific Laboratory Supplies | EACH | EUR 7125 |
Universal Oven UF1060 |
|||
| OVE8348 | Scientific Laboratory Supplies | EACH | EUR 12519.48 |
Universal Oven UN30plus |
|||
| OVE8350 | Scientific Laboratory Supplies | EACH | EUR 2020.08 |
Universal Oven UN55plus |
|||
| OVE8352 | Scientific Laboratory Supplies | EACH | EUR 2204.76 |
Universal Oven UN75plus |
|||
| OVE8354 | Scientific Laboratory Supplies | EACH | EUR 2453.28 |
Universal Oven UN110plus |
|||
| OVE8356 | Scientific Laboratory Supplies | EACH | EUR 2812.38 |
Universal Oven UN260plus |
|||
| OVE8358 | Scientific Laboratory Supplies | EACH | EUR 4189.5 |
Universal Oven UN450plus |
|||
| OVE8360 | Scientific Laboratory Supplies | EACH | EUR 5400.18 |
Universal Oven UN750plus |
|||
| OVE8362 | Scientific Laboratory Supplies | EACH | EUR 7083.96 |
Universal Oven UF30plus |
|||
| OVE8366 | Scientific Laboratory Supplies | EACH | EUR 2131.8 |
Universal Oven UF55plus |
|||
| OVE8368 | Scientific Laboratory Supplies | EACH | EUR 2530.8 |
Universal Oven UF75plus |
|||
| OVE8370 | Scientific Laboratory Supplies | EACH | EUR 2789.58 |
Universal Oven UF110plus |
|||
| OVE8372 | Scientific Laboratory Supplies | EACH | EUR 3218.22 |
Universal Oven UF160plus |
|||
| OVE8374 | Scientific Laboratory Supplies | EACH | EUR 4009.38 |
Universal Oven UF260plus |
|||
| OVE8376 | Scientific Laboratory Supplies | EACH | EUR 4753.8 |
Universal Oven UF450plus |
|||
| OVE8378 | Scientific Laboratory Supplies | EACH | EUR 6065.94 |
Universal Oven UF750plus |
|||
| OVE8380 | Scientific Laboratory Supplies | EACH | EUR 8708.46 |
Universal Oven UF1060plus |
|||
| OVE8382 | Scientific Laboratory Supplies | EACH | EUR 13331.16 |
18mmMag Universal ScrewCap |
|||
| CHR3460 | Scientific Laboratory Supplies | PK1000 | EUR 950.76 |
Universal Indicator Solution |
|||
| UN10025 | Scientific Laboratory Supplies | 2.5L | EUR 165.3 |
Universal Indicator Solution |
|||
| UN1005 | Scientific Laboratory Supplies | 50ML | EUR 17.1 |
Universal Indicator Solution |
|||
| UN101 | Scientific Laboratory Supplies | 100ML | EUR 18.24 |
Universal Indicator Solution |
|||
| UN1025 | Scientific Laboratory Supplies | 25L | EUR 650.94 |
Universal Indicator Solution |
|||
| UN105 | Scientific Laboratory Supplies | 500ML | EUR 90.06 |
Universal Indicator Solution |
|||
| UNB1010 | Scientific Laboratory Supplies | 10L | EUR 627 |
IKA Universal Attachment |
|||
| SHA2016 | Scientific Laboratory Supplies | EACH | EUR 947.34 |
Universal V1-Puck |
|||
| M-CP-111-021 | MiTeGen | 1 PUCK | EUR 350 |
|
Description: Universal V1-Puck |
|||
Tumour Marker MUC1 (S1.1) , DNA Aptamer, Biotinylated |
|||
| AD-152-B | Alpha Diagnostics | Custom | Ask for price |
Tumour Marker MUC1 (S1.1) , DNA Aptamer, unlabeled |
|||
| AD-152-U | Alpha Diagnostics | Custom | Ask for price |
500-10000bp DNA Marker, Ready-to-use |
|||
| M101R-1 | Bio Basic | 100loading, 100prep | EUR 110.11 |
500-10000bp DNA Marker, Ready-to-use |
|||
| M101R-2 | Bio Basic | 5x100loading, 500prep | EUR 263.58 |
100-1500bp DNA Marker, Ready-to-use |
|||
| M102R-1 | Bio Basic | 100loading, 100prep | EUR 108.02 |
100-1500bp DNA Marker, Ready-to-use |
|||
| M102R-2 | Bio Basic | 5x100loading, 500prep | EUR 258.36 |
Lambda DNA/HindIII Marker, Ready-to-use |
|||
| M104R-1 | Bio Basic | 100loading, 100prep | EUR 78.79 |
Lambda DNA/HindIII Marker, Ready-to-use |
|||
| M104R-2 | Bio Basic | 5x100loading, 500prep | EUR 149.78 |
100-1500bp DNA Marker, Ready-to-use |
|||
| M107R-1 | Bio Basic | 100loading, 100prep | EUR 110.11 |
100-1500bp DNA Marker, Ready-to-use |
|||
| M107R-2 | Bio Basic | 5x100loading, 500prep | EUR 263.58 |
50-1500bp DNA Marker, Ready-to-use |
|||
| M109-A | Bio Basic | 100loading, 100prep | EUR 110.11 |
50-1500bp DNA Marker, Ready-to-use |
|||
| M109-B | Bio Basic | 5x100loading, 500prep | EUR 263.58 |
100-1200bp DNA Marker, Ready-to-use |
|||
| GM333 | Bio Basic | 50loading, 50prep | EUR 80.88 |
100-1200bp DNA Marker, Ready-to-use |
|||
| GM334 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
100-2000bp DNA Marker, Ready-to-use |
|||
| GM335 | Bio Basic | 50loading, 50prep | EUR 80.88 |
100-2000bp DNA Marker, Ready-to-use |
|||
| GM336 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
200-1500bp DNA Marker, Ready-to-use |
|||
| GM337 | Bio Basic | 50loading, 50prep | EUR 80.88 |
200-1500bp DNA Marker, Ready-to-use |
|||
| GM338 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
200-2000bp DNA Marker, Ready-to-use |
|||
| GM339 | Bio Basic | 50loading, 50prep | EUR 80.88 |
200-2000bp DNA Marker, Ready-to-use |
|||
| GM340 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
300-2500bp DNA Marker, Ready-to-use |
|||
| GM341 | Bio Basic | 50loading, 50prep | EUR 80.88 |
300-2500bp DNA Marker, Ready-to-use |
|||
| GM342 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
100-1000bp DNA Marker, Ready-to-use |
|||
| GM343 | Bio Basic | 50loading, 50prep | EUR 96.54 |
100-1000bp DNA Marker, Ready-to-use |
|||
| GM345 | Bio Basic | 50loading, 50prep | EUR 101.76 |
100-1500bp DNA Marker, Ready-to-use |
|||
| SGM01 | Bio Basic | 50loading, 50prep | EUR 80.88 |
100-1500bp DNA Marker, Ready-to-use |
|||
| SGM02 | Bio Basic | 5x50loading, 250prep | EUR 153.96 |
PCR plate-foil universal |
|||
| A28216 | GeneOn | 1x100 pcs | EUR 112.8 |
PCR plate-foil universal |
|||
| A28216L | GeneOn | 5x100 pcs | EUR 372 |
Universal Casein Diluent/Blocker |
|||
| 85R-108 | Fitzgerald | 250 ml | EUR 276 |
|
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates |
|||
Universal Protein Precipitating Reagent |
|||
| BSP012 | Bio Basic | 100preps | EUR 97.58 |
5min Universal Ligation Mix |
|||
| C311-01 | Vazyme | 50 rxn | EUR 256.8 |
5min Universal Ligation Mix |
|||
| C311-02 | Vazyme | 100 rxn | EUR 339.6 |
Universal Full Growth Medium |
|||
| TM001 | Neuromics | 500 ml | EUR 403.2 |
Universal Ohaus Plug GB |
|||
| 46001779 | Scientific Laboratory Supplies | EACH | EUR 13.91 |
Universal tray for LSB12 |
|||
| BAT1062 | Scientific Laboratory Supplies | EACH | EUR 263.34 |
Universal tray for LSB18 |
|||
| BAT1064 | Scientific Laboratory Supplies | EACH | EUR 275.88 |
2 Universal Clip Plates |
|||
| MIX5190 | Scientific Laboratory Supplies | EACH | EUR 90.06 |
Azo Universal spray 500ml |
|||
| CLE1996 | Scientific Laboratory Supplies | EACH | EUR 5.18 |
Universal Active Charcoal Filter |
|||
| FUM1146 | Scientific Laboratory Supplies | EACH | EUR 465.12 |
Universal Attachment for SHA20702 |
|||
| SHA2074 | Scientific Laboratory Supplies | EACH | EUR 2370.06 |
IKA AS4000.1 Universal Attachment |
|||
| SHA2612 | Scientific Laboratory Supplies | EACH | EUR 1189.02 |
Universal Platform Innova S44i |
|||
| SHA3072 | Scientific Laboratory Supplies | EACH | EUR 1279.08 |
Grant Universal platform 270x195mm |
|||
| SHA7932 | Scientific Laboratory Supplies | EACH | EUR 159.6 |
Universal Pipette Holder Assorted |
|||
| PIP0164 | Scientific Laboratory Supplies | EACH | EUR 44.57 |
18mm Mag Universal ScrewCap |
|||
| CHR3466 | Scientific Laboratory Supplies | PK1000 | EUR 898.32 |
If the molar ratio of plasmid vector to focus on DNA is simply too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the international DNA and the variety of inserts in every recombinant clone should all the time be validated by restriction endonuclease mapping or another means.
