Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an vital goal for the event of therapeutic engineered micro organism, benefiting from the wealth of data of E. coli biology and ease of manipulation. Bacterial artificial biology tasks generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nonetheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or therapy.
EcN natively accommodates plasmids pMUT1 and pMUT2, which haven’t any identified operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable useful materials into the extracellular house.
We then show that the engineered plasmids have been maintained in micro organism because the engineered micro organism move by the mouse GI tract with out choice, and that the secretion system stays useful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the speedy improvement of therapeutic EcN micro organism.
Amplification of EBNA-1 by a single-plasmid vector-based gene amplification system in HEK293 cells as an environment friendly transient gene expression system
Our earlier work confirmed that there’s a limitation in the usage of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification programs in dhfr-non-deficient HEK293 cells, as endogenous dhfr might intervene with the amplification course of. Within the current examine, we efficiently generated Epstein-Barr virus nuclear antigen-1 (EBNA-1)-amplified HEK293 cells in a dhfr-non-deficient HEK293 cell background utilizing a single-plasmid vector-based gene amplification system with shRNA concentrating on the three’-UTR of endogenous dhfr. The introduction of this shRNA effectively downregulated the expression of endogenous dhfr within the HEK293 cells with out affecting exogenous dhfr expression.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this examine have been larger than these obtained from EBNA-1-amplified clones that have been generated utilizing the traditional amplification in our earlier examine. In step with earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell progress or transfection effectivity.
As well as, the N-glycan profiles within the Fc-fusion protein produced utilizing this transient gene expression (TGE) system weren’t affected by EBNA-1 amplification. These outcomes point out the potential utility of EBNA-1-amplified mammalian cells, developed utilizing a single-plasmid vector-based gene amplification system, for environment friendly protein manufacturing.
Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA
The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and subsequently are largely demanded within the biopharmaceutical manufacturing. Nonetheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing price. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this examine, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.
Plasmids DNA with totally different proportion of SC, open-circular, and linearized varieties have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.
Nonetheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This examine demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, at the least underneath our manufacturing course of.
Cloning in Plasmid Vectors: Blunt-Finish Cloning
This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “right” ligation merchandise when cloning blunt-ended goal fragments, the 2 parts of DNA within the ligation response should be current at an applicable ratio.
 Methylamp Universal Methylated DNA Preparation Kit |
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| P-1019 | EpiGentek | 40 µg | EUR 347.25 |
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Description: The best epigenetics products |
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 100 BP DNA LADDER, 500UL PER KIT |
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| M-DNA-100BP | CORNING | 1/pk | EUR 73 |
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Description: Bioscience Mol Bio; DNA Ladder |
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 1 KB DNA LADDER, 500UL PER KIT |
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| M-DNA-1KB | CORNING | 1/pk | EUR 70 |
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Description: Bioscience Mol Bio; DNA Ladder |
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 VAHTS Universal DNA Library Prep Kit for MGI |
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| NDM607-01 | Vazyme | 24 rxn | EUR 733 |
VAHTS Universal DNA Library Prep Kit for MGI |
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| NDM607-02 | Vazyme | 96 rxn | EUR 2136 |
 2K DNA Marker |
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| 20-abx098045 | Abbexa |
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 5K DNA Marker |
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| 20-abx098048 | Abbexa |
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 15K DNA Marker |
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| 20-abx098049 | Abbexa |
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 1Kb DNA Marker |
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| 20-abx098050 | Abbexa |
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 100bp DNA Marker |
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| 20-abx098052 | Abbexa |
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100bp DNA Marker |
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| D1100-050 | GenDepot | 50㎍ | EUR 165 |
100bp DNA Marker |
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| D1100-250 | GenDepot | 5X50㎍ | EUR 550 |
1Kb DNA Marker |
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| D1101-050 | GenDepot | 50㎍ | EUR 131 |
1Kb DNA Marker |
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| D1101-250 | GenDepot | 5X50㎍ | EUR 300 |
 DL5000 DNA Marker |
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| MD102-01 | Vazyme | 250 μl | EUR 107 |
DL5000 DNA Marker |
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| MD102-02 | Vazyme | 500 μl | EUR 113 |
 DL15000 DNA Marker |
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| MD103-01 | Vazyme | 250 μl | EUR 109 |
DL15000 DNA Marker |
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| MD103-02 | Vazyme | 500 μl | EUR 115 |
 VAHTS Universal DNA Library Prep Kit for Illumina V3 |
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| ND607-01 | Vazyme | 24 rxn | EUR 441 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 |
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| ND607-02 | Vazyme | 96 rxn | EUR 1405 |
 VAHTS Universal Pro DNA Library Prep Kit for Illumina |
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| ND608-01 | Vazyme | 24 rxn | EUR 465 |
VAHTS Universal Pro DNA Library Prep Kit for Illumina |
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| ND608-02 | Vazyme | 96 rxn | EUR 1549 |
 VAHTS Universal Plus DNA Library Prep Kit for Illumina |
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| ND617-01 | Vazyme | 24 rxn | EUR 492 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina |
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| ND617-02 | Vazyme | 96 rxn | EUR 1605 |
 VAHTS Universal DNA Library Prep Kit for Ion Torrent |
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| ND701-01 | Vazyme | 24 rxn | EUR 733 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent |
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| ND701-02 | Vazyme | 96 rxn | EUR 2136 |
 VAHTS Universal Pro DNA Library Prep Kit for MGI |
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| NDM608-01 | Vazyme | 24 rxn | EUR 756 |
VAHTS Universal Pro DNA Library Prep Kit for MGI |
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| NDM608-02 | Vazyme | 96 rxn | EUR 2284 |
 VAHTS Universal Plus DNA Library Prep Kit for MGI |
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| NDM617-01 | Vazyme | 24 rxn | EUR 824 |
VAHTS Universal Plus DNA Library Prep Kit for MGI |
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| NDM617-02 | Vazyme | 96 rxn | EUR 2408 |
 UNIVERSAL PLATFORM |
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| 480144 | CORNING | 1/pk | EUR 269 |
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Description: Lab Equipment; Constant Temperature Equipment |
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 1Kb Plus DNA Marker |
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| 20-abx098051 | Abbexa |
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 DL2000 Plus DNA Marker |
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| MD101-01 | Vazyme | 250 μl | EUR 107 |
DL2000 Plus DNA Marker |
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| MD101-02 | Vazyme | 500 μl | EUR 113 |
 Linear Kinetoplast DNA Marker |
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| TG2018-1 | TopoGen | 10 ug | EUR 179 |
 Decatenated Kinetoplast DNA Marker |
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| TG2020-1 | TopoGen | 10 ug | EUR 190 |
 Relaxed pRYG DNA Marker |
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| TG2025-1 | TopoGen | 10 ug | EUR 268 |
 Linear pRYG DNA Marker |
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| TG2028-1 | TopoGen | 10 ug | EUR 179 |
 1 Kb Ladder DNA Marker |
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| FYD001-500UL | Yeastern Biotech | 20 µg/ 500 µl | Ask for price |
 100 bp Ladder DNA Marker |
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| FYD002-500UL | Yeastern Biotech | 30 µg/ 500 µl | Ask for price |
 50 bp Ladder DNA Marker |
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| FYD004-500UL | Yeastern Biotech | 32.5 µg/ 500 µl | Ask for price |
 Universal Detachment Solution |
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| AD002 | Neuromics | 100 ml | EUR 148 |
 Universal Spring Platform |
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| H1000-P-SP | BenchMark | 1 PC | EUR 323.39 |
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 Universal Coating Solution |
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| AC002 | Neuromics | 20 ml | EUR 297 |
 Universal Neutralization Buffer |
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| NB001 | Neuromics | 100 ml | EUR 135 |
 UNIVERSAL BEER AGAR |
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| U21-100-10kg | Alphabiosciences | 10 kg | EUR 1524 |
UNIVERSAL BEER AGAR |
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| U21-100-2kg | Alphabiosciences | 2kg | EUR 370 |
UNIVERSAL BEER AGAR |
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| U21-100-500g | Alphabiosciences | 500 g | EUR 137 |
 UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-10kg | Alphabiosciences | 10 kg | EUR 749 |
UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-2Kg | Alphabiosciences | 2 Kg | EUR 202 |
UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-500g | Alphabiosciences | 500 g | EUR 91 |
 Universal qPCR Mixture |
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| QPCR1000-UNIV | Bio Basic | 4X1.25ml | EUR 276.2 |
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) VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
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| ND607-03 | Vazyme | 24 rxn | EUR 429 |
) VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
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| ND607-04 | Vazyme | 96 rxn | EUR 1354 |
 Image Green? 100 bp DNA Marker |
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| M1155-500 | Biovision | EUR 294 | |
 Image Green? 1 kb DNA Marker |
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| M1156-500 | Biovision | EUR 294 | |
 50-500bp Low Range DNA Marker |
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| GM305 | Bio Basic | 200loading, 200prep | EUR 114.82 |
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 500-10000bp DNA Marker, Original Form |
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| M101O-1 | Bio Basic | 100loading, 100prep | EUR 91.76 |
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500-10000bp DNA Marker, Original Form |
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| M101O-2 | Bio Basic | 5x100loading, 500prep | EUR 219.65 |
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 100-1500bp DNA Marker, Original Form |
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| M102O-1 | Bio Basic | 100loading, 100prep | EUR 90.02 |
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100-1500bp DNA Marker, Original Form |
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| M102O-2 | Bio Basic | 5x100loading, 500prep | EUR 215.3 |
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100-1500bp DNA Marker, Original Form |
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| M107O-1 | Bio Basic | 100loading, 100prep | EUR 91.76 |
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100-1500bp DNA Marker, Original Form |
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| M107O-2 | Bio Basic | 5x100loading, 500prep | EUR 219.65 |
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) 2K Plus DNA Marker (8 linear double-stranded DNA bands) |
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| 20-abx098046 | Abbexa |
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) 2K Plus DNA Marker (9 linear double-stranded DNA bands) |
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| 20-abx098047 | Abbexa |
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) 100bp Plus DNA Marker (12 linear double-stranded DNA bands) |
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| 20-abx098053 | Abbexa |
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) 100bp Plus DNA Marker (14 linear double-stranded DNA bands) |
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| 20-abx098054 | Abbexa |
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 5min Universal Ligation Mix |
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| C311-01 | Vazyme | 50 rxn | EUR 214 |
5min Universal Ligation Mix |
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| C311-02 | Vazyme | 100 rxn | EUR 283 |
 Universal Casein Diluent/Blocker |
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| 85R-108 | Fitzgerald | 250 ml | EUR 230 |
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Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates |
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 PCR plate-foil universal |
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| A28216 | GeneOn | 1x100 pcs | EUR 94 |
PCR plate-foil universal |
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| A28216L | GeneOn | 5x100 pcs | EUR 310 |
 Universal Full Growth Medium |
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| TM001 | Neuromics | 500 ml | EUR 336 |
 Universal Protein Precipitating Reagent |
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| BSP012 | Bio Basic | 100preps | EUR 81.32 |
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 , DNA Aptamer, Biotinylated) Tumour Marker MUC1 (S1.1) , DNA Aptamer, Biotinylated |
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| AD-152-B | Alpha Diagnostics | Custom | Ask for price |
 , DNA Aptamer, unlabeled) Tumour Marker MUC1 (S1.1) , DNA Aptamer, unlabeled |
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| AD-152-U | Alpha Diagnostics | Custom | Ask for price |
 100-1200bp DNA Marker, Ready-to-use |
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| GM333 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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100-1200bp DNA Marker, Ready-to-use |
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| GM334 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 100-2000bp DNA Marker, Ready-to-use |
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| GM335 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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100-2000bp DNA Marker, Ready-to-use |
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| GM336 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 200-1500bp DNA Marker, Ready-to-use |
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| GM337 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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200-1500bp DNA Marker, Ready-to-use |
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| GM338 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 200-2000bp DNA Marker, Ready-to-use |
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| GM339 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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200-2000bp DNA Marker, Ready-to-use |
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| GM340 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 300-2500bp DNA Marker, Ready-to-use |
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| GM341 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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300-2500bp DNA Marker, Ready-to-use |
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| GM342 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 100-1000bp DNA Marker, Ready-to-use |
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| GM343 | Bio Basic | 50loading, 50prep | EUR 80.45 |
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100-1000bp DNA Marker, Ready-to-use |
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| GM345 | Bio Basic | 50loading, 50prep | EUR 84.8 |
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 500-10000bp DNA Marker, Ready-to-use |
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| M101R-1 | Bio Basic | 100loading, 100prep | EUR 91.76 |
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500-10000bp DNA Marker, Ready-to-use |
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| M101R-2 | Bio Basic | 5x100loading, 500prep | EUR 219.65 |
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 100-1500bp DNA Marker, Ready-to-use |
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| M102R-1 | Bio Basic | 100loading, 100prep | EUR 90.02 |
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100-1500bp DNA Marker, Ready-to-use |
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| M102R-2 | Bio Basic | 5x100loading, 500prep | EUR 215.3 |
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 Lambda DNA/HindIII Marker, Ready-to-use |
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| M104R-1 | Bio Basic | 100loading, 100prep | EUR 65.66 |
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Lambda DNA/HindIII Marker, Ready-to-use |
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| M104R-2 | Bio Basic | 5x100loading, 500prep | EUR 124.82 |
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100-1500bp DNA Marker, Ready-to-use |
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| M107R-1 | Bio Basic | 100loading, 100prep | EUR 91.76 |
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100-1500bp DNA Marker, Ready-to-use |
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| M107R-2 | Bio Basic | 5x100loading, 500prep | EUR 219.65 |
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 50-1500bp DNA Marker, Ready-to-use |
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| M109-A | Bio Basic | 100loading, 100prep | EUR 91.76 |
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50-1500bp DNA Marker, Ready-to-use |
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| M109-B | Bio Basic | 5x100loading, 500prep | EUR 219.65 |
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100-1500bp DNA Marker, Ready-to-use |
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| SGM01 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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100-1500bp DNA Marker, Ready-to-use |
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| SGM02 | Bio Basic | 5x50loading, 250prep | EUR 128.3 |
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 UNIVERSAL LINEAR 4 PLACE STAND |
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| AP-USD4 | CORNING | 1/pk | EUR 70 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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 HotTaq Probe qPCR Universal Mix |
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| BT11004 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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 HotTaq EvaGreen qPCR Universal Mix |
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| BT11104 | Bioatlas | 250rxn | EUR 138 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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 Universal IHC Diluent/Blocker/Stabilizer |
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| 85R-1021 | Fitzgerald | 1 liter | EUR 692 |
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Description: Universal Diluent/Blocker/Stabilizer for use in IHC |
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 VAHTS Universal Plus Fragmentation Module |
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| N209-01 | Vazyme | 24 rxn | EUR 376 |
VAHTS Universal Plus Fragmentation Module |
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| N209-02 | Vazyme | 96 rxn | EUR 1022 |
 Universal 3' miRNA Reverse Primer |
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| MPH00000 | ABM | 150 ul / 10 uM | EUR 70 |
 100UL UNIVERSAL FIT FILTER TIPS |
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| TF-100 | CORNING | 1000/pk | EUR 418 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 1000UL UNIVERSAL FIT FILTER TIPS |
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| TF-1000 | CORNING | 1000/pk | EUR 232 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 200UL UNIVERSAL FIT FILTER TIPS |
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| TF-200 | CORNING | 1000/pk | EUR 418 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 300UL UNIVERSAL FIT FILTER TIPS |
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| TF-350 | CORNING | 1000/pk | EUR 418 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 50UL UNIVERSAL FIT FILTER TIPS |
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| TF-50 | CORNING | 1000/pk | EUR 418 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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 , DNA Aptamer, FITC labeled) Tumour Marker MUC1 (S1.1) , DNA Aptamer, FITC labeled |
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| AD-152-F | Alpha Diagnostics | Custom | Ask for price |
 100bp DNA Marker Plus-50, Ready-to-use |
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| D1103-050 | GenDepot | 500 ul | EUR 151 |
100bp DNA Marker Plus-50, Ready-to-use |
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| D1103-100 | GenDepot | 2x500 ul | EUR 192 |
 100bp PCR Ranger DNA Marker, 100bp-To-3Kb |
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| D1108-100 | GenDepot | 1ml | EUR 220 |
100bp PCR Ranger DNA Marker, 100bp-To-3Kb |
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| D1108-200 | GenDepot | 2x1ml | EUR 309 |
 1Kb PCR Ranger DNA Marker, 750bp-To-20Kb |
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| D1109-100 | GenDepot | 1ml | EUR 188 |
1Kb PCR Ranger DNA Marker, 750bp-To-20Kb |
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| D1109-400 | GenDepot | 4x1ml | EUR 388 |
 200-2000bp DNA Marker Plus, Ready-to-use |
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| GM401 | Bio Basic | 50loading, 50prep | EUR 60.44 |
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 100-5000bp DNA Marker Plus, Ready-to-use |
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| SGM03 | Bio Basic | 50loading, 50prep | EUR 67.4 |
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 UNIVERSAL STAND FOR FOUR PIPETTORS, RED |
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| AP-USD4-P | CORNING | 1/pk | EUR 69 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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 UNIVERSAL STAND FOR SIX PIPETTORS, TRANSPARENT |
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| AP-USD6 | CORNING | 1/pk | EUR 85 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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 Human Adult Tissues Protein lysate, Universal |
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| HUUL-4565 | Alpha Diagnostics | 1 mg | EUR 524 |
) Universal miRNA qPCR Primer (10 µM) |
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| abx298006-200ul | Abbexa | 200 ul | EUR 189 |
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 Antibody) Universal Stress Protein F (uspF) Antibody |
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| 20-abx300670 | Abbexa |
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 Enhanced Universal IHC Diluent/Blocker/Stabilizer |
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| 85R-1021E | Fitzgerald | 100 ml | EUR 187 |
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Description: Enhanced Universal Diluent/Blocker/Stabilizer for use in IHC |
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) Universal TT epitope P2 (830-844) |
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| 5-02048 | CHI Scientific | 4 x 1mg | Ask for price |
 UNIVERSAL CAROUSEL RACK FOR SIX PIPETTES |
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| 4078 | CORNING | 1/pk | EUR 132 |
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Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
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 UNIVERSAL LINEAR RACK FOR SIX PIPETTES |
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| 4079 | CORNING | 1/pk | EUR 70 |
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Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
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 STAT Universal Animal IHC KIT-small |
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| 329ANK-20 | Innovex | Small Kit | EUR 717 |
 STAT Universal Animal IHC KIT-large |
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| 329ANK-60 | Innovex | Large Kit | EUR 1088 |
 ADPsensor? Universal Kinase Activity Assay Kit |
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| K212-100 | Biovision | EUR 582 | |
 Dog Adult Tissues Protein lysate, Universal |
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| DOUL-4568 | Alpha Diagnostics | 1 mg | EUR 524 |
 Chicken Adult Tissues Protein lysate, Universal |
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| CHUL-4567 | Alpha Diagnostics | 1 mg | EUR 524 |
 UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET |
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| MOTO-SP-PWR | CORNING | 1/pk | EUR 61 |
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Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
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 Mouse Adult Tissues Protein lysate, Universal |
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| MOUL-4566 | Alpha Diagnostics | 1 mg | EUR 524 |
 Monkey Adult Tissues Protein lysate, Universal |
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| MKUL-4569 | Alpha Diagnostics | 1 mg | EUR 524 |
 Universal Protein Lysate: Human Normal Tissues |
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| P4234565 | Biochain | 4x0.5 mg | EUR 403 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Universal Protein Lysate: Human Normal Tissues |
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| P4234565-1 | Biochain | 2x0.5 mg | EUR 285 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Universal Protein Lysate: Mouse Normal Tissues |
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| P4334566 | Biochain | 4x0.5 mg | EUR 403 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Universal Protein Lysate: Mouse Normal Tissues |
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| P4334566-1 | Biochain | 2x0.5 mg | EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Universal Protein Lysate: Rat Normal Tissues |
|||
| P4434567 | Biochain | 4x0.5 mg | EUR 403 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
Universal Protein Lysate: Rat Normal Tissues |
|||
| P4434567-1 | Biochain | 2x0.5 mg | EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Universal Protein Lysate: Monkey Normal Tissues |
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| P4534565 | Biochain | 4x0.5 mg | EUR 403 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
Universal Protein Lysate: Monkey Normal Tissues |
|||
| P4534565-1 | Biochain | 2x0.5 mg | EUR 285 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
|||
 Universal Protein Lysate: Dog Normal Tissues |
|||
| P4734565 | Biochain | 4x0.5 mg | EUR 403 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Universal Protein Lysate: Dog Normal Tissues |
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| P4734565-1 | Biochain | 2x0.5 mg | EUR 285 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Universal Protein Lysate: Chicken Normal Tissues |
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| P4C34565 | Biochain | 4x0.5 mg | EUR 403 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Universal Protein Lysate: Chicken Normal Tissues |
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| P4C34565-1 | Biochain | 2x0.5 mg | EUR 285 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Rat Adult Tissues Protein lysate, Universal |
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| RTUL-4570 | Alpha Diagnostics | 1 mg | EUR 524 |
 AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
 ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646 |
 Universal RNA from Human Normal Tissues |
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| R4234565 | Biochain | 4x100 ug | EUR 628 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Human Normal Tissues |
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| R4234565-1 | Biochain | 2x100 ug | EUR 390 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Universal RNA from Mouse Normal Tissues |
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| R4334566 | Biochain | 4x100 ug | EUR 530 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Mouse Normal Tissues |
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| R4334566-1 | Biochain | 2x100 ug | EUR 311 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Universal RNA from Rat Normal Tissues |
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| R4434567 | Biochain | 4x100 ug | EUR 530 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Rat Normal Tissues |
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| R4434567-1 | Biochain | 2x100 ug | EUR 311 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Universal RNA from Monkey Normal Tissues |
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| R4534565 | Biochain | 4x100 ug | EUR 628 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Monkey Normal Tissues |
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| R4534565-1 | Biochain | 2x100 ug | EUR 390 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Universal RNA from Dog Normal Tissues |
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| R4734565 | Biochain | 4x100 ug | EUR 628 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Dog Normal Tissues |
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| R4734565-1 | Biochain | 2x100 ug | EUR 390 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Universal RNA from Chicken Normal Tissues |
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| R4C34565 | Biochain | 4x100 ug | EUR 628 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Universal RNA from Chicken Normal Tissues |
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| R4C34565-1 | Biochain | 2x100 ug | EUR 390 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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) pSMPUW Universal Lentiviral Expression Vector (Promoterless) |
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| VPK-211 | Cell Biolabs | 10 µg | EUR 624 |
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Description: Clone your gene of interest and a gene-specific promoter into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293T or 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer. |
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 ViraSafe Universal Lentiviral Expression System, Ecotropic |
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| VPK-211-ECO | Cell Biolabs | 1 kit | EUR 1163 |
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Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
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 ViraSafe Universal Lentiviral Expression System, Pantropic |
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| VPK-211-PAN | Cell Biolabs | 1 kit | EUR 1163 |
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Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin. |
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 RAPAd® Universal Adenoviral Expression System |
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| VPK-250 | Cell Biolabs | 1 kit | EUR 1036 |
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Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression. |
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 100bp PCR Ranger DNA Marker, 100bp-To-3Kb, Concentrated |
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| D2108-500 | GenDepot | 10x50ug | EUR 901 |
 1.5 kb to 48.5 kb High Range DNA Marker |
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| E255 | Bio Basic | 150ug, 150ug | EUR 315.35 |
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 Antibody (HRP)) Universal Stress Protein F (USPF) Antibody (HRP) |
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| 20-abx300671 | Abbexa |
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 Antibody (FITC)) Universal Stress Protein F (USPF) Antibody (FITC) |
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| 20-abx300672 | Abbexa |
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 Antibody (Biotin)) Universal Stress Protein F (USPF) Antibody (Biotin) |
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| 20-abx300673 | Abbexa |
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 EZBlock? Universal Protease and Phosphatase Inhibitor Cocktail |
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| K283-1 | Biovision | EUR 218 | |
If the molar ratio of plasmid vector to focus on DNA is simply too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the international DNA and the variety of inserts in every recombinant clone should all the time be validated by restriction endonuclease mapping or another means.