Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an vital goal for the event of therapeutic engineered micro organism, benefiting from the wealth of data of E. coli biology and ease of manipulation. Bacterial artificial biology tasks generally make the most of engineered plasmid vectors, that are easy to engineer and might reliably obtain excessive ranges of protein expression. Nonetheless, plasmids usually require antibiotics for upkeep, and the administration of an antibiotic is commonly incompatible with in vivo experimentation or therapy.
EcN natively accommodates plasmids pMUT1 and pMUT2, which haven’t any identified operate however are secure inside the micro organism. Right here, we describe the event of the pMUT plasmids into a strong platform for engineering EcN for in vivo experimentation, alongside a CRISPR-Cas9 system to take away the native plasmids. We systematically engineered each pMUT plasmids to include choice markers, fluorescent markers, temperature delicate expression, and curli secretion programs to export a customizable useful materials into the extracellular house.
We then show that the engineered plasmids have been maintained in micro organism because the engineered micro organism move by the mouse GI tract with out choice, and that the secretion system stays useful, exporting functionalized curli proteins into the intestine. Our plasmid system presents a platform for the speedy improvement of therapeutic EcN micro organism.
Amplification of EBNA-1 by a single-plasmid vector-based gene amplification system in HEK293 cells as an environment friendly transient gene expression system
Our earlier work confirmed that there’s a limitation in the usage of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification programs in dhfr-non-deficient HEK293 cells, as endogenous dhfr might intervene with the amplification course of. Within the current examine, we efficiently generated Epstein-Barr virus nuclear antigen-1 (EBNA-1)-amplified HEK293 cells in a dhfr-non-deficient HEK293 cell background utilizing a single-plasmid vector-based gene amplification system with shRNA concentrating on the three’-UTR of endogenous dhfr. The introduction of this shRNA effectively downregulated the expression of endogenous dhfr within the HEK293 cells with out affecting exogenous dhfr expression.
The downregulation of endogenous dhfr improved the effectivity of EBNA-1 amplification, as evidenced by a comparability with the amplification extent in cells missing shRNA expression on the similar MTX focus. The EBNA-1 expression ranges from the EBNA-1-amplified clones chosen on this examine have been larger than these obtained from EBNA-1-amplified clones that have been generated utilizing the traditional amplification in our earlier examine. In step with earlier research, EBNA-1 amplification improved the manufacturing of the Fc-fusion protein by a selected protein productiveness (qp)-enhancing impact, slightly than by bettering cell progress or transfection effectivity.
As well as, the N-glycan profiles within the Fc-fusion protein produced utilizing this transient gene expression (TGE) system weren’t affected by EBNA-1 amplification. These outcomes point out the potential utility of EBNA-1-amplified mammalian cells, developed utilizing a single-plasmid vector-based gene amplification system, for environment friendly protein manufacturing.
Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA
The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and subsequently are largely demanded within the biopharmaceutical manufacturing. Nonetheless, manufacturing of high-purity SC plasmid DNA would lead to excessive manufacturing price. The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this examine, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.
Plasmids DNA with totally different proportion of SC, open-circular, and linearized varieties have been ready utilizing the thermal denaturation technique, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%. Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer irrespective of the packaging cell line was.
Nonetheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This examine demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, at the least underneath our manufacturing course of.
Cloning in Plasmid Vectors: Blunt-Finish Cloning
This protocol describes procedures for cloning blunt-ended DNA fragments into linearized plasmid vectors. To acquire the utmost variety of “right” ligation merchandise when cloning blunt-ended goal fragments, the 2 parts of DNA within the ligation response should be current at an applicable ratio.
Genorise® Universal DNA Mini Kit 100 |
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| GR101076-100 | Genorise Scientific | 100 | EUR 357 |
Genorise® Universal DNA Mini Kit 200 |
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| GR101077 | Genorise Scientific | 200 | EUR 567 |
Genorise® Universal DNA Mini Kit 500 |
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| GR101077-500 | Genorise Scientific | 500 | EUR 1272 |
Methylamp Universal Methylated DNA Kit |
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| P-1011 | EpiGentek |
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VAHTS Universal DNA Library Prep Kit for MGI |
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| NDM607-01 | Vazyme | 24 rxn | EUR 879.6 |
VAHTS Universal DNA Library Prep Kit for MGI |
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| NDM607-02 | Vazyme | 96 rxn | EUR 2563.2 |
VAHTS Universal DNA library Prep Kit for MGI V4 |
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| NDM610-01 | Vazyme | 24 rxns | EUR 235.2 |
VAHTS Universal DNA library Prep Kit for MGI V4 |
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| NDM610-02 | Vazyme | 96 rxns | EUR 880 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 |
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| ND607-01 | Vazyme | 24 rxn | EUR 529.2 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 |
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| ND607-02 | Vazyme | 96 rxn | EUR 1686 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent |
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| ND701-01 | Vazyme | 24 rxn | EUR 879.6 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent |
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| ND701-02 | Vazyme | 96 rxn | EUR 2563.2 |
VAHTS Universal DNA Library Prep Kit for Illumina V4 |
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| ND610-01 | Vazyme | 24 rxns | EUR 235.2 |
VAHTS Universal DNA Library Prep Kit for Illumina V4 |
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| ND610-02 | Vazyme | 96 rxns | EUR 880 |
Methylamp Universal Methylated DNA Preparation Kit |
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| P-1019 | EpiGentek | 40 µg | EUR 416.7 |
EpiMag High Throughput DNA Isolation Universal Kit |
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| P-1022-96 | EpiGentek | 96 Samples | EUR 227.7 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent V2 |
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| ND702-01 | Vazyme | 24 rxns | EUR 235.2 |
VAHTS Universal DNA Library Prep Kit for Ion Torrent V2 |
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| ND702-02 | Vazyme | 96 rxns | EUR 880 |
VAHTS Universal Pro DNA Library Prep Kit for MGI |
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| NDM608-01 | Vazyme | 24 rxn | EUR 907.2 |
VAHTS Universal Pro DNA Library Prep Kit for MGI |
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| NDM608-02 | Vazyme | 96 rxn | EUR 2740.8 |
VAHTS Universal Plus DNA Library Prep Kit for MGI |
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| NDM617-01 | Vazyme | 24 rxn | EUR 988.8 |
VAHTS Universal Plus DNA Library Prep Kit for MGI |
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| NDM617-02 | Vazyme | 96 rxn | EUR 2889.6 |
VAHTS Universal Plus DNA Library Prep Kit for MGI V2 |
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| NDM627-01 | Vazyme | 24 rxns | EUR 250 |
VAHTS Universal Plus DNA Library Prep Kit for MGI V2 |
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| NDM627-02 | Vazyme | 96 rxns | EUR 920 |
VAHTS Universal Pro DNA Library Prep Kit for Illumina |
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| ND608-01 | Vazyme | 24 rxn | EUR 558 |
VAHTS Universal Pro DNA Library Prep Kit for Illumina |
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| ND608-02 | Vazyme | 96 rxn | EUR 1858.8 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina |
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| ND617-01 | Vazyme | 24 rxn | EUR 590.4 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina |
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| ND617-02 | Vazyme | 96 rxn | EUR 1926 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
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| ND607-03 | Vazyme | 24 rxn | EUR 514.8 |
VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free) |
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| ND607-04 | Vazyme | 96 rxn | EUR 1624.8 |
Universal platform |
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| INC1112 | Scientific Laboratory Supplies | EACH | EUR 1434 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 |
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| ND627-01 | Vazyme | 24 rxns | EUR 250 |
VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 |
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| ND627-02 | Vazyme | 96 rxns | EUR 920 |
Universal Attachment |
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| MIX1320 | Scientific Laboratory Supplies | EACH | EUR 286.8 |
Universal V1-Puck |
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| M-CP-111-021 | MiTeGen | 1 PUCK | EUR 350 |
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Description: Universal V1-Puck |
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Universal Oven UN30 |
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| OVE8314 | Scientific Laboratory Supplies | EACH | EUR 1672.8 |
Universal Oven UN55 |
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| OVE8316 | Scientific Laboratory Supplies | EACH | EUR 1952.4 |
Universal Oven UN75 |
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| OVE8318 | Scientific Laboratory Supplies | EACH | EUR 2028 |
Universal Oven UF30 |
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| OVE8332 | Scientific Laboratory Supplies | EACH | EUR 1911.6 |
Universal Oven UF55 |
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| OVE8334 | Scientific Laboratory Supplies | EACH | EUR 2306.4 |
Universal Oven UF75 |
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| OVE8336 | Scientific Laboratory Supplies | EACH | EUR 2433.6 |
UNIVERSAL BEER AGAR |
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| U21-100-10kg | Alphabiosciences | 10 kg | EUR 1828.8 |
UNIVERSAL BEER AGAR |
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| U21-100-2kg | Alphabiosciences | 2kg | EUR 444 |
UNIVERSAL BEER AGAR |
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| U21-100-500g | Alphabiosciences | 500 g | EUR 164.4 |
Grant Universal Tray |
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| BAT4622 | Scientific Laboratory Supplies | EACH | EUR 314.4 |
Universal AC adapter |
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| PIP3214 | Scientific Laboratory Supplies | EACH | EUR 78 |
Universal Oven UN110 |
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| OVE8320 | Scientific Laboratory Supplies | EACH | EUR 2598 |
Universal Oven UN160 |
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| OVE8322 | Scientific Laboratory Supplies | EACH | EUR 3133.2 |
Universal Oven UN260 |
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| OVE8324 | Scientific Laboratory Supplies | EACH | EUR 4148.4 |
Universal Oven UN450 |
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| OVE8326 | Scientific Laboratory Supplies | EACH | EUR 5490 |
Universal Oven UN750 |
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| OVE8328 | Scientific Laboratory Supplies | EACH | EUR 7356 |
Universal Oven UF110 |
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| OVE8338 | Scientific Laboratory Supplies | EACH | EUR 2876.4 |
Universal Oven UF160 |
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| OVE8340 | Scientific Laboratory Supplies | EACH | EUR 3740.4 |
Universal Oven UF260 |
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| OVE8342 | Scientific Laboratory Supplies | EACH | EUR 4768.8 |
Universal Oven UF450 |
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| OVE8344 | Scientific Laboratory Supplies | EACH | EUR 6244.8 |
Universal Oven UF750 |
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| OVE8346 | Scientific Laboratory Supplies | EACH | EUR 8275.2 |
Universal Spring Tray |
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| BAT7177 | Scientific Laboratory Supplies | EACH | EUR 298.8 |
Universal Oven UF1060 |
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| OVE8348 | Scientific Laboratory Supplies | EACH | EUR 15282 |
NBS Universal Platform |
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| SHA4050 | Scientific Laboratory Supplies | EACH | EUR 1459.2 |
NBS Universal Platform |
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| SHA4070 | Scientific Laboratory Supplies | EACH | EUR 1059.6 |
NBS Universal Platform |
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| SHA4232 | Scientific Laboratory Supplies | EACH | EUR 580.8 |
NBS Universal Platform |
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| SHA4256 | Scientific Laboratory Supplies | EACH | EUR 711.6 |
NBS Universal Platform |
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| SHA4272 | Scientific Laboratory Supplies | EACH | EUR 1129.2 |
NBS Universal Platform |
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| SHA4290 | Scientific Laboratory Supplies | EACH | EUR 1519.2 |
Universal qPCR Mixture |
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| QPCR1000-UNIV | Bio Basic | 4X1.25ml | EUR 331.44 |
Mains adaptor (universal) |
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| ARG1248 | Scientific Laboratory Supplies | EACH | EUR 135.78 |
Universal Oven UN30plus |
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| OVE8350 | Scientific Laboratory Supplies | EACH | EUR 2390.4 |
Universal Oven UN55plus |
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| OVE8352 | Scientific Laboratory Supplies | EACH | EUR 2608.8 |
Universal Oven UN75plus |
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| OVE8354 | Scientific Laboratory Supplies | EACH | EUR 2902.8 |
Universal Oven UF30plus |
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| OVE8366 | Scientific Laboratory Supplies | EACH | EUR 2523.6 |
Universal Oven UF55plus |
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| OVE8368 | Scientific Laboratory Supplies | EACH | EUR 2752.8 |
Universal Oven UF75plus |
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| OVE8370 | Scientific Laboratory Supplies | EACH | EUR 3300 |
Loopster Universal Disc |
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| MIX1080 | Scientific Laboratory Supplies | EACH | EUR 180 |
Universal Oven UN110plus |
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| OVE8356 | Scientific Laboratory Supplies | EACH | EUR 3327.6 |
Universal Oven UN260plus |
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| OVE8358 | Scientific Laboratory Supplies | EACH | EUR 4956 |
Universal Oven UN450plus |
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| OVE8360 | Scientific Laboratory Supplies | EACH | EUR 6390 |
Universal Oven UN750plus |
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| OVE8362 | Scientific Laboratory Supplies | EACH | EUR 8228.4 |
Universal Oven UF110plus |
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| OVE8372 | Scientific Laboratory Supplies | EACH | EUR 3807.6 |
Universal Oven UF160plus |
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| OVE8374 | Scientific Laboratory Supplies | EACH | EUR 4743.6 |
Universal Oven UF260plus |
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| OVE8376 | Scientific Laboratory Supplies | EACH | EUR 5660.4 |
Universal Oven UF450plus |
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| OVE8378 | Scientific Laboratory Supplies | EACH | EUR 7177.2 |
Universal Oven UF750plus |
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| OVE8380 | Scientific Laboratory Supplies | EACH | EUR 9790.8 |
IKA Universal Attachment |
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| SHA2016 | Scientific Laboratory Supplies | EACH | EUR 997.2 |
Universal attachment IKA |
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| SHA2210 | Scientific Laboratory Supplies | EACH | EUR 783.6 |
Universal Ohaus Plug GB |
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| 46001779 | Scientific Laboratory Supplies | EACH | EUR 16.8 |
Universal Stratus Adapter |
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| MIC6036 | Scientific Laboratory Supplies | EACH | EUR 592.8 |
Universal Oven UF1060plus |
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| OVE8382 | Scientific Laboratory Supplies | EACH | EUR 16224 |
Universal Spring Platform |
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| MIX7184 | Scientific Laboratory Supplies | EACH | EUR 885.6 |
Universal Spring Platform |
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| H1000-P-SP | Benchmark Scientific | 1 PC | EUR 640.9 |
Universal Spring Platform |
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| H1010-P-SP | Benchmark Scientific | 1 each | EUR 677.5 |
Universal tray for LSB12 |
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| BAT1062 | Scientific Laboratory Supplies | EACH | EUR 300 |
Universal tray for LSB18 |
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| BAT1064 | Scientific Laboratory Supplies | EACH | EUR 314.4 |
Azo Universal spray 500ml |
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| CLE1996 | Scientific Laboratory Supplies | EACH | EUR 10.29 |
2 Universal Clip Plates |
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| MIX5190 | Scientific Laboratory Supplies | EACH | EUR 110.4 |
18mmMag Universal ScrewCap |
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| CHR3460 | Scientific Laboratory Supplies | PK1000 | EUR 1339.2 |
Universal (Aluminum) Platform |
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| MIX1322 | Scientific Laboratory Supplies | EACH | EUR 139.2 |
Universal Multichannel Stand |
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| F161417 | Scientific Laboratory Supplies | EACH | EUR 33.6 |
Universal Indicator Solution |
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| UN10025 | Scientific Laboratory Supplies | 2.5L | EUR 205.2 |
Universal Indicator Solution |
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| UN1005 | Scientific Laboratory Supplies | 50ML | EUR 21.31 |
Universal Indicator Solution |
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| UN101 | Scientific Laboratory Supplies | 100ML | EUR 22.8 |
Universal Indicator Solution |
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| UN1025 | Scientific Laboratory Supplies | 25L | EUR 812.08 |
Universal Indicator Solution |
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| UN105 | Scientific Laboratory Supplies | 500ML | EUR 111.6 |
Universal Indicator Solution |
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| UNB1010 | Scientific Laboratory Supplies | 10L | EUR 782.05 |
PCR plate-foil universal |
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| A28216 | GeneOn | 1x100 pcs | EUR 112.8 |
PCR plate-foil universal |
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| A28216L | GeneOn | 5x100 pcs | EUR 372 |
AED Universal Wall Bracket |
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| SAF1128 | Scientific Laboratory Supplies | EACH | EUR 107.06 |
Wire Rack 2x 6 Universal |
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| STA20020 | Scientific Laboratory Supplies | EACH | EUR 36 |
Universal Detachment Solution |
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| AD002 | Neuromics | 100 ml | EUR 177.6 |
UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-10kg | Alphabiosciences | 10 kg | EUR 898.8 |
UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-2Kg | Alphabiosciences | 2 Kg | EUR 242.4 |
UNIVERSAL PREENRICHMENT BROTH |
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| U21-101-500g | Alphabiosciences | 500 g | EUR 109.2 |
5min Universal Ligation Mix |
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| C311-01 | Vazyme | 50 rxn | EUR 256.8 |
5min Universal Ligation Mix |
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| C311-02 | Vazyme | 100 rxn | EUR 339.6 |
18mm Mag Universal ScrewCap |
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| CHR3466 | Scientific Laboratory Supplies | PK1000 | EUR 1266 |
18mm Mag Universal ScrewCap |
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| CHR3468 | Scientific Laboratory Supplies | PK1000 | EUR 954 |
Pack of 10 universal seals |
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| WAT4870 | Scientific Laboratory Supplies | PK10 | EUR 214.12 |
Universal Full Growth Medium |
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| TM001 | Neuromics | 500 ml | EUR 403.2 |
Universal V1-Puck; Qnty 7 |
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| M-CP-111-021-7 | MiTeGen | 7 PUCKS | EUR 2420 |
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Description: Universal V1-Puck; Qnty 7 |
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10 × Universal Primer Mix (UPM) |
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| RA102-01 | Vazyme | 100 rxns (5 μl/rxn) | EUR 58 |
Universal Neutralization Buffer |
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| NB001 | Neuromics | 100 ml | EUR 162 |
Glass universal 30ml sep cap |
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| 127030CS | Scientific Laboratory Supplies | PK500 | EUR 246 |
Universal Indicator pH 1-14 |
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| RRSP4455-D | Atom Scientific | 500ml | EUR 8.63 |
Universal Indicator pH 1-14 |
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| RRSP4455-E | Atom Scientific | 1L | EUR 15.86 |
Universal Indicator pH 1-14 |
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| RRSP4455-F | Atom Scientific | 2.5L | EUR 30.22 |
Finntip 250 Universal 0.5-250ul 1000 Per bag |
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| PIP3612 | Scientific Laboratory Supplies | PK1000 | EUR 94.8 |
Universal Platform Innova S44i |
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| SHA3072 | Scientific Laboratory Supplies | EACH | EUR 1449.6 |
Nunc Universal Lid PS Sterile |
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| 250002 | Scientific Laboratory Supplies | PK60 | EUR 294 |
Universal Platform 28 X 33cm |
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| SHA6028 | Scientific Laboratory Supplies | EACH | EUR 223.2 |
Universal Platform 33 X 33cm |
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| SHA6032 | Scientific Laboratory Supplies | EACH | EUR 319.2 |
Universal Platform 46 X 46cm |
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| SHA6034 | Scientific Laboratory Supplies | EACH | EUR 382.8 |
Universal Platform 46 X 61cm |
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| SHA6036 | Scientific Laboratory Supplies | EACH | EUR 430.8 |
Universal Platform 61 X 61cm |
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| SHA6038 | Scientific Laboratory Supplies | EACH | EUR 1034.4 |
Universal Platform 61 X 91cm |
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| SHA6062 | Scientific Laboratory Supplies | EACH | EUR 1310.4 |
A5 Boots Vinyl sole UNIVERSAL |
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| SAF4750 | Scientific Laboratory Supplies | 100PR | EUR 655.41 |
A5 Boots Vinyl foot UNIVERSAL |
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| SAF4756 | Scientific Laboratory Supplies | 100PR | EUR 825.14 |
Universal TT epitope P2 (830-844) |
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| 5-02048 | CHI Scientific | 4 x 1mg | Ask for price |
IKA AS4000.1 Universal Attachment |
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| SHA2612 | Scientific Laboratory Supplies | EACH | EUR 1251.6 |
SLS Pipette Tip Universal 1-200ul Loose |
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| PIP7812 | Scientific Laboratory Supplies | PK1000 | EUR 26.63 |
50UL UNIVERSAL FIT FILTER TIPS |
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| TF-50 | CORNING | 1000/pk | EUR 501.6 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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Universal Casein Diluent/Blocker |
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| 85R-108 | Fitzgerald | 250 ml | EUR 276 |
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Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates |
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Universal Active Charcoal Filter |
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| FUM1146 | Scientific Laboratory Supplies | EACH | EUR 637.2 |
UNIVERSAL LINEAR 4 PLACE STAND |
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| AP-USD4 | CORNING | 1/pk | EUR 84 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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Sterile universal 30ml no label |
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| 128A/IRR | Scientific Laboratory Supplies | PK400 | EUR 196.8 |
HotTaq Probe qPCR Universal Mix |
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| BT11004 | Bioatlas | 250rxn | EUR 147.6 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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Universal scissors length 150 mm |
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| DD76147 | Scientific Laboratory Supplies | EACH | EUR 36 |
10 x Universal Blocking Buffer |
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| GR103005 | Genorise Scientific | 200 mL | EUR 129 |
30ml Universal PS Printed Label |
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| SLE7502 | Scientific Laboratory Supplies | PK400 | EUR 116.4 |
100UL UNIVERSAL FIT FILTER TIPS |
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| TF-100 | CORNING | 1000/pk | EUR 501.6 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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1000UL UNIVERSAL FIT FILTER TIPS |
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| TF-1000 | CORNING | 1000/pk | EUR 278.4 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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200UL UNIVERSAL FIT FILTER TIPS |
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| TF-200 | CORNING | 1000/pk | EUR 501.6 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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300UL UNIVERSAL FIT FILTER TIPS |
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| TF-350 | CORNING | 1000/pk | EUR 501.6 |
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Description: Filter Tip; Filter Tips Pipette - Axygen |
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Universal Pipette Holder Assorted |
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| PIP0164 | Scientific Laboratory Supplies | EACH | EUR 55.44 |
Universal Attachment for SHA20702 |
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| SHA2074 | Scientific Laboratory Supplies | EACH | EUR 2494.8 |
Grant Universal platform 270x195mm |
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| SHA7932 | Scientific Laboratory Supplies | EACH | EUR 176.4 |
Universal Lids N Ste w/oRings |
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| PCR0380 | Scientific Laboratory Supplies | PK100 | EUR 226.8 |
Finnpipette F3 1-10ul Universal |
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| PIP0101 | Scientific Laboratory Supplies | EACH | EUR 427.2 |
Finnpipette F3 2-20ul Universal |
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| PIP0103 | Scientific Laboratory Supplies | EACH | EUR 427.2 |
Universal Pipette Stand Clear 4 |
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| PIP0386 | Scientific Laboratory Supplies | EACH | EUR 63.6 |
Universal Pipette Stand Clear 6 |
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| PIP0396 | Scientific Laboratory Supplies | EACH | EUR 88.8 |
Platform universal flat-bed 4 |
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| SHA7952 | Scientific Laboratory Supplies | EACH | EUR 638.4 |
Universal RNA - Rat Normal Tissues |
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| R4434567 | Biochain | 4x100 ug | EUR 635 |
Universal RNA - Rat Normal Tissues |
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| R4434567-1 | Biochain | 2x100 ug | EUR 347 |
Universal RNA - Dog Normal Tissues |
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| R4734565 | Biochain | 4x100 ug | EUR 766 |
Universal RNA - Dog Normal Tissues |
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| R4734565-1 | Biochain | 2x100 ug | EUR 450 |
Universal tiered rack blue colour |
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| DD64012 | Scientific Laboratory Supplies | EACH | EUR 37.2 |
Universal 3' miRNA Reverse Primer |
|||
| MPH00000 | ABM | 150 ul / 10 uM | EUR 84 |
Universal Pipette Stand Orange 4 |
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| PIP0384 | Scientific Laboratory Supplies | EACH | EUR 63.6 |
Ohaus Support Stand Universal-H |
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| STI2048 | Scientific Laboratory Supplies | EACH | EUR 308.4 |
Universal control tissue microarray |
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| CTRL141 | TissueArray | each | EUR 54 |
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Description: Universal control tissue microarray, 7 types of Immune System normal tissues, 7 cases/14 cores |
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Nunc Universal Lid PS Non Sterile |
|||
| 250003 | Scientific Laboratory Supplies | PK60 | EUR 260.4 |
Universal miRNA qPCR Primer (10 µM) |
|||
| abx298006-200ul | Abbexa | 200 ul | EUR 226.8 |
Select 30ml Universal PS No Label |
|||
| SLE7500 | Scientific Laboratory Supplies | PK400 | EUR 96 |
HotTaq EvaGreen qPCR Universal Mix |
|||
| BT11104 | Bioatlas | 250rxn | EUR 165.6 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
|||
Universal tiered rack green colour |
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| DD64013 | Scientific Laboratory Supplies | EACH | EUR 37.2 |
Adapter plates for Universal rotor |
|||
| E5910753004 | Scientific Laboratory Supplies | PK2 | EUR 296.4 |
Universal RNA - Human Normal Tissues |
|||
| R4234565 | Biochain | 4x100 ug | EUR 766 |
Universal RNA - Human Normal Tissues |
|||
| R4234565-1 | Biochain | 2x100 ug | EUR 450 |
If the molar ratio of plasmid vector to focus on DNA is simply too excessive, then the ligation response might generate an undesirable variety of round empty plasmids, each monomeric and polymeric; if too low, the ligation response might generate an extra of linear and round homopolymers and heteropolymers of various sizes, orientations, and compositions. Because of this, the orientation of the international DNA and the variety of inserts in every recombinant clone should all the time be validated by restriction endonuclease mapping or another means.
