Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to several types of stress. Genome enhancing of Pputida largely depends on homologous recombination occasions, assisted by helper plasmid-based expression of genes encoding DNA modifying enzymes. Plasmid curing from chosen isolates is probably the most tedious and time-consuming step of this process, and implementing generally used strategies to this finish in Pputida (e.g. temperature-sensitive replicons) is usually impractical.

To deal with this difficulty, we have now developed a toolbox for each target- and self-curing of plasmid DNA in Pseudomonas species. Our technique allows plasmid-curing in a easy cultivation step by combining in vivo digestion of vectors by the I-SceI homing nuclease with artificial management of plasmid replication, triggered by the addition of an inexpensive chemical inducer (3-methylbenzoate) to the medium. The system shows an effectivity of vector curing >90% and the screening of plasmid-free clones is drastically facilitated by means of fluorescent markers that may be chosen in accordance with the appliance supposed.

Moreover, fast genome engineering of Pputida utilizing self-curing plasmids is demonstrated via genome discount of the platform pressure EM42 by eliminating all genes encoding β-lactamases, the catabolic ben gene cluster, and the pyoverdine synthesis equipment. Physiological characterization of the ensuing streamlined pressure, Pputida SEM10, revealed advantageous options that could possibly be exploited for metabolic engineering.

The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.

For many years, various plasmid vectors have been repeatedly developed for molecular cloning of DNA fragment within the bacterial host cell Escherichia coli. Even with deliberate performances in vector preparation, the cloning approaches nonetheless face inevitable background colonies, or false optimistic clones, which may be arisen from intact or self-ligated plasmid molecules. To help in such drawback, two plasmids, pBS2ndd and pBS3ndd, which proof against ampicillin and kanamycin respectively, had been developed on this examine as extra advantageous cloning vector. The plasmids carry ndd, a deadly gene from bacteriophage T4 coding for nucleoid disruption protein that binds to the host chromosome and progressively kill the cell.

The lethal toxicity of Ndd inhibits host cells that receive intact or ndd-religated vector from rising, which leads to low background and dramatically reduces the trouble for choice of recombinants. Furthermore, their equivalent a number of cloning web site was designed to help varied cloning methods. Digestion of plasmids with XcmI permits for in vitro T/A ligation, whereas with EcoRV permits blunt-end ligation, with functionality of blue-white colony screening. In vivo homologous recombination cloning can be utilizable by amplification of insert fragments utilizing primers containing homology arms and transformation into succesful E. coli strains.

To reveal their benefits, the plasmids had been used to clone PCR product samples for DNA sequencing with low-background and versatile cloning methods. Such fast and cost-effective cloning procedures are additionally proposed right here. Lastly, the cloning for protein expression with blue-white choice was additionally potential utilizing egfp as a mannequin regulated by lac and T7 promoters on the plasmid or different build-in promoters with the insert.

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Building of a plasmid vector containing epidermal development issue receptor and C-Jun shRNA.

The target of this examine was to assemble a plasmid vector for EGFR-hm-1 and C-Junh-825 small interfering RNA (siRNA). EGFR-hm-1 and C-Jun-hm-825 oligonucleotide fragments had been synthesized and quick hairpin RNA (shRNA) had been amplified by PCR. Plasmids had been remoted from E. coli TOP10 bacterium by restriction enzyme digestion utilizing pst1 and BamH1 and oligonucleotide fragments had been cloned into the pSilencer plasmid containing the U6 promoter.

Recombinant clones had been generated by remodeling JM109 competent cells with plasmid vectors containing shRNA molecules. 58 base-paired EGFR-hm-1 and 59 base-paired C-Jun-hm-825 oligonucleotide fragments had been remoted. The fragments had been 100% homologous with human sequences out there on GenBank. The recombinant pSilencer1.zero vector containing a 58-bp EGFR-hm-1 and 59-bp C-Jun-hm-825 fragment was constructed. These vectors have the potential for use as remedy to fight pores and skin photoaging underneath UV publicity.

Characterization of a cryptic plasmid remoted from Lactobacillus casei CP002616 and development of shuttle vectors based mostly on its replicon.

The cryptic plasmid pLC2W was remoted from Lactobacillus casei CP002616. Nucleotide sequence evaluation revealed that four putative open studying frames (ORF) had been answerable for DNA replication. 4 Escherichia coli-Lactobacillus shuttle vectors had been constructed utilizing completely different lengths of the pLC2W replicon to establish the shortest practical replicon. The size of the pLC2W replicon didn’t have an effect on the soundness of the plasmids. Inexperienced fluorescent protein (GFP) as a reporter was expressed efficiently in a number of lactobacilli utilizing our constructed vectors.

The outcomes urged that the expression vectors pUE-F0GFP and pUE-F1GFP are potential molecular instruments for heterologous gene cloning and expression in lactobacilli. Furthermore, 2 plasmid-curing strategies had been used to remove pLC2W from L. casei. We detected no distinction between L. casei CP002616 and L. casei CP002616 pLC2WΔ-IC (mutant pressure cured by plasmid incompatibility technique) in manufacturing of exopolysaccharide (EPS) or acid.

Protein A Magnetic Beads

6507-1
EUR 314.4

Protein G Magnetic Beads

6517-1
EUR 314.4

Protein L Magnetic Beads

6537-1
EUR 352.8

Anti-Flag magnetic beads

B26101 1 mL
EUR 579.6
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26102 5 mL
EUR 1742.4
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-HA magnetic beads

B26201 1 mL
EUR 579.6
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA magnetic beads

B26202 5 mL
EUR 1742.4
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-Myc magnetic beads

B26301 1 mL
EUR 579.6
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-Myc magnetic beads

B26302 5 mL
EUR 1742.4
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-HA Magnetic Beads

HY-K0201 1 mL
EUR 334.8

Protein A Magnetic Beads

HY-K0203 1 mL
EUR 148.8

Protein G Magnetic Beads

HY-K0204 5 mL
EUR 445.2

Anti-Flag Magnetic Beads

HY-K0207 1 mL
EUR 570

Protein A/G Magnetic Beads

6527-1
EUR 352.8

Protein A/G Magnetic Beads

HY-K0202 1 mL
EUR 157.2

Anti-c-Myc Magnetic Beads

HY-K0206 5 mL
EUR 1368

Size Selection DNA Beads

20-abx298001
  • EUR 260.40
  • EUR 477.60
  • EUR 1663.20
  • 1 ml
  • 5 ml
  • 60 ml

VAHTS DNA Clean Beads

N411-01 5 ml
EUR 259.2

VAHTS DNA Clean Beads

N411-02 60 ml
EUR 634.8

VAHTS DNA Clean Beads

N411-03 450 ml
EUR 3055.2

Protein A/G/L Magnetic Beads

6547-1
EUR 405.6

Protein A/G Magnetic Beads for IP

B23201 1 ml
EUR 158.4
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A/G Magnetic Beads for IP

B23202 5 ml
EUR 558
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A Beads (1-2mg/ml)

B0001-10 10ml
EUR 486

Protein A Beads (1-2mg/ml)

B0001-25 25ml
EUR 973.2

Protein A Beads (1-2mg/ml)

B0001-5 5ml
EUR 291.6

Protein G Beads (1-2mg/ml)

B0002-10 10ml
EUR 729.6

Protein G Beads (1-2mg/ml)

B0002-25 25ml
EUR 1472.4

Protein G Beads (1-2mg/ml)

B0002-5 5ml
EUR 426

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Bottle Centrifuge Pp 450 Ml

3141-0500 PK4
EUR 152.76

Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb

AE037 50 ul
EUR 211.2

Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)

M-CO-501 24 glass beads
EUR 71
Description: Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube)

FAB & Beads(Ballotini Beads)

MED1042 PK50
EUR 91.2

Goat Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0003-10 10ml
EUR 681.6

Goat Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0003-25 25ml
EUR 1228.8

Goat Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0003-5 5ml
EUR 426

Goat Anti-Mouse IgG Beads (1-1.5mg/ml)

B0004-10 10ml
EUR 644.4

Goat Anti-Mouse IgG Beads (1-1.5mg/ml)

B0004-25 25ml
EUR 1228.8

Goat Anti-Mouse IgG Beads (1-1.5mg/ml)

B0004-5 5ml
EUR 400.8

Rabbit Anti-Human IgG Beads (1-1.5mg/ml)

B0005-10 10ml
EUR 949.2

Rabbit Anti-Human IgG Beads (1-1.5mg/ml)

B0005-25 25ml
EUR 2008.8

Rabbit Anti-Human IgG Beads (1-1.5mg/ml)

B0005-5 5ml
EUR 547.2

Rabbit Anti-Rat IgG Beads (1-1.5mg/ml)

B0006-10 10ml
EUR 949.2

Rabbit Anti-Rat IgG Beads (1-1.5mg/ml)

B0006-25 25ml
EUR 2008.8

Rabbit Anti-Rat IgG Beads (1-1.5mg/ml)

B0006-5 5ml
EUR 547.2

Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0007-10 10ml
EUR 644.4

Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0007-25 25ml
EUR 1228.8

Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml)

B0007-5 5ml
EUR 400.8

Mouse Anti-Human IgG Beads (1-1.5mg/ml)

B0008-10 10ml
EUR 949.2

Mouse Anti-Human IgG Beads (1-1.5mg/ml)

B0008-25 25ml
EUR 2008.8

Mouse Anti-Human IgG Beads (1-1.5mg/ml)

B0008-5 5ml
EUR 547.2

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

Circulating DNA Kit (with Magnetic Stand)

abx098869-50rxns 50 rxns
EUR 510

Circulating DNA Kit (with Magnetic Stand)

abx298026-50rxns 50 rxns
EUR 510

Cyanine7 tetrazine, 25 mg

450 25 mg
EUR 615.6

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns 50 rxns
EUR 360

Viral DNA / RNA Kit (with Magnetic Stand)

abx098871-50rxns 50 rxns
EUR 477.6

Viral DNA/RNA Kit (with Magnetic Stand)

abx298029-50rxns 50 rxns
EUR 477.6

Bulk Beads

D1131-01 1 PC
EUR 285.76

Bulk Beads

D1131-05 1 PC
EUR 285.76

Bulk Beads

D1131-10 1 PC
EUR 492.47

Bulk Beads

D1132-01TP 1 PC
EUR 492.47

Bulk Beads

D1132-05TP 1 PC
EUR 492.47

Bulk Beads

D1132-10TP 1 PC
EUR 492.47

Bulk Beads

D1132-15TP 1 PC
EUR 492.47

Bulk Beads

D1132-30TP 1 PC
EUR 492.47

Bulk Beads

D1132-60 1 PC
EUR 289.93

Bulk Beads

D1133-28 1 PC
EUR 112.45

Exorose Beads

EXOB-100 100 ml
EUR 127.2

Exorose Beads

EXOB-25 25 ml
EUR 69.6

Exorose Beads

EXOB-250 250 ml
EUR 225.6

Latex Beads

abx291003-1L 1 L
EUR 2798.4

RNA Beads

20-abx298002
  • EUR 260.40
  • EUR 477.60
  • EUR 1663.20
  • 1 ml
  • 5 ml
  • 60 ml

Saline & Beads

MED1064 PK50
EUR 91.2

T4 DNA Polymerase (5,000 U/ml)

RK20539 25 U Ask for price

V5 agarose beads

AE029-100ul 100 ul Ask for price

V5 agarose beads

AE029-200ul 200 ul
EUR 273.6

V5 agarose beads

AE029-20ul 20 ul Ask for price

V5 agarose beads

AE029-50ul 50 ul Ask for price

Streptavidin-Sepharose Beads

6565-10
EUR 836.4

Streptavidin-Sepharose Beads

6565-100 Ask for price

Streptavidin-Sepharose Beads

6565-2
EUR 274.8

Streptavidin-Sepharose Beads

6565-5
EUR 483.6

EZEnrich? Polyubiquitin Beads

6568-300
EUR 444

Thrombin Sepharose Beads

7925-1
EUR 261.6

Thrombin Sepharose Beads

7925-25
EUR 2114.4

Thrombin Sepharose Beads

7925-5
EUR 836.4

Plasmin Sepharose Beads

7926-1
EUR 261.6

Plasmin Sepharose Beads

7926-25
EUR 2114.4

Plasmin Sepharose Beads

7926-5
EUR 836.4

Urokinase Sepharose Beads

7927-1
EUR 261.6

Urokinase Sepharose Beads

7927-25
EUR 2114.4

Urokinase Sepharose Beads

7927-5
EUR 836.4

Immobilized Catalase Beads

7931-1
EUR 183.6

Immobilized Catalase Beads

7931-10
EUR 705.6

Calmodulin-Sepharose Beads

7934-1
EUR 183.6

Calmodulin-Sepharose Beads

7934-10
EUR 574.8

Calmodulin-Sepharose Beads

7934-5
EUR 405.6

Refill Glass Beads

B1201-BEAD 1 PC
EUR 141.34

Glass Beads 0.1mm

GB01 1pack
EUR 129.6
Description: Glass beads. 0.1mm. 1 lb. Non-sterile.

Glass Beads 0.5mm

GB05 1pack
EUR 123.6
Description: Glass beads. 0.5mm. 1 lb. Non-sterile.

Glass Beads 1.0mm

GB10 1pack
EUR 123.6
Description: Glass beads. 1.0mm. 1 lb. Non-sterile.

Sodium hydroxide, Beads

SB6789 500g
EUR 73.21

RanBP1 Agarose Beads

STA-421 400 µg
EUR 686.4
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Protect beads green

SWA1151 PK80
EUR 103.74

Protect Beads White

SWA1155 PK80
EUR 103.74

Protect Beads Blue

SWA1158 PK80
EUR 103.74

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

illustra Ready-To-Go RT-PCR Beads 0.5 ml tubes 100 rxns

27926601 EACH
EUR 414.96

illustra Ready-To-Go RT-PCR Beads 0.2 ml tubes 96 rxns

27926701 EACH
EUR 386.46

illustra PuReTaq Ready-To-Go PCR Beads 0.5 ml tubes 100 rxns

27955801 EACH
EUR 159.6

Calibration Beads for MoFlo

B28479
EUR 346.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

VAHTS mRNA Capture Beads

N401-01 24 rxn
EUR 171.6

VAHTS mRNA Capture Beads

N401-02 96 rxn
EUR 306

VAHTS RNA Clean Beads

N412-01 5 ml
EUR 286.8

VAHTS RNA Clean Beads

N412-02 40 ml
EUR 495.6

VAHTS RNA Clean Beads

N412-03 450 ml
EUR 4860

Stainless Steel Beads 0.2mm

SSB02 1pack
EUR 229.2
Description: Stainless steel beads. 0.2mm. 1 lb. Non-sterile.

Stainless Steel Beads 0.5mm

SSB05 1pack
EUR 229.2
Description: Stainless steel beads. 0.5mm. 1 lb. Non-sterile.

Stainless Steel Beads 1.6mm

SSB16 1pack
EUR 304.8
Description: Stainless steel beads. 1.6mm. 1 lb. Non-sterile.

Stainless Steel Beads 2.3mm

SSB23 1pack
EUR 260.4
Description: Stainless steel beads. 2.3mm. 1 lb. Non-sterile.

Stainless Steel Beads 3.2mm

SSB32 1pack
EUR 220.8
Description: Stainless steel beads. 3.2mm. 1 lb. Non-sterile.

Stainless Steel Beads 4.8mm

SSB48 1pack
EUR 220.8
Description: Stainless steel beads. 4.8mm. 1 lb. Non-sterile.

Stainless Steel Beads 6.0mm

SSB60 1pack
EUR 223.2
Description: Stainless steel beads. 6.0mm. 1 lb. Non-sterile.

PAK1 PBD Agarose Beads

STA-411 400 ?g
EUR 686.4
Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Rhotekin RBD Agarose Beads

STA-412 400 ?g
EUR 686.4
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

RalGDS RBD Agarose Beads

STA-418 400 µg
EUR 686.4
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

GGA3 PBD Agarose Beads

STA-419 400 µg
EUR 686.4
Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

RalBP1 PBD Agarose Beads

STA-420 400 µg
EUR 686.4
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Zirconium Oxide Beads 0.15mm

ZrOB015 1pack
EUR 189.6
Description: Fused Zirconium Oxide beads . 0.15mm. 1 lb. Non-sterile.

Zirconium Oxide Beads 0.5mm

ZrOB05 1pack
EUR 189.6
Description: Zirconium Oxide beads . 0.5mm. 1 lb. Non-sterile.

Zirconium Oxide Beads 1.0mm

ZrOB10 1pack
EUR 189.6
Description: Zirconium Oxide beads . 1.0mm. 1 lb. Non-sterile.

Zirconium Oxide Beads 2.0mm

ZrOB20 1pack
EUR 189.6
Description: Zirconium Oxide beads . 2.0mm. 1 lb. Non-sterile.

Zirconium Oxide Beads 2.5mm

ZrOB25 1pack
EUR 189.6
Description: Zirconium Oxide beads . 2.5mm. 1 lb. Non-sterile.

Zirconium Silicate Beads 0.5mm

ZSB05 1pack
EUR 146.4
Description: Zirconium Silicate beads . 0.5mm. 1 lb. Non-sterile.

Zirconium Silicate Beads 1.0mm

ZSB10 1pack
EUR 146.4
Description: Zirconium Silicate beads . 1.0mm. 1 lb. Non-sterile.

Rtg Rapd Analysis Beads

27950001 EACH
EUR 804.84

Glass Calibration Beads 4mm

SV144 500G
EUR 14.71

Protect Beads Mixed Colours

SWA1147 PK80
EUR 103.74

Cot I human DNA conc. >=1,1 mg / ml

3001 500 µg
EUR 140.4

Magnetic Stirrer

abx725038-1Unit 1 Unit
EUR 518.4

Magnetic Stand

abx799001-1Unit 1 Unit
EUR 309.6

Magnetic Separator

B23803 2 mL/200 uL/15 mL
EUR 291.6
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time.

Magnetic Separator

MagSep1-1 1/pk
EUR 132

Magnetic Stand

HY-K0200 200 μL-2 mL-15 mL
EUR 456

Magnetic Stirrer

H3760-SE 1 PC
EUR 121.85

Magnetic Stirrer

H4000-S-E 1 PC
EUR 97.22

RNase-Free Calf Thymus DNA, 1 mg/mL (5x1mL)

9-31065
  • EUR 206.40
  • EUR 566.40
  • 1tube
  • 5tubes
Description: Minimum order quantity: 1 unit of 5tubes

cfPure® Cell Free DNA Extraction Kit, 100 ml

K5011610 1 kit
EUR 537.6

cfPure® Cell Free DNA Extraction Kit, 250 ml

K5011625 1 kit
EUR 897.6

Calibration Beads for MoFlo Astrios

B27034
EUR 346.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Glass Beads 0.1mm RNase Free

GB01-RNA 1pack
EUR 115.2
Description: Glass beads. 0.1mm. 4mL. RNase free.

Glass Beads 0.5mm RNase Free

GB05-RNA 1pack
EUR 115.2
Description: Glass beads. 0.5mm. 4mL. RNase free.

Stainless Steel UFO Beads 3.5mm

SSUFO35 1pack
EUR 229.2
Description: Stainless steel UFO beads. 3.5mm. 50mL. Non-sterile.

Stainless Steel UFO Beads 5.6mm

SSUFO56 1pack
EUR 225.6
Description: Stainless steel UFO beads. 5.6mm. 50mL. Non-sterile.

Raf-1 RBD Agarose Beads

STA-410 400 ?g
EUR 686.4
Description: Raf-1 RBD Agarose Beads selectively pull down the active form of Ras. Beads are colored to allow for a visual check. These are the same beads supplied with our Ras Activation Assays. 400 µg.

500GM Glass beads 2mm 500gm

GS/2 500G
EUR 15.28

Flexi-Block + Beads Double Width

BLO2162 EACH
EUR 283.86

Flexi-Block + Beads Triple Width

BLO2163 EACH
EUR 428.64

Protect Beads Green PP Tray

SWA1164 PK100
EUR 166.44

Protect Beads Red PP Tray

SWA1166 PK100
EUR 166.44

Protect Beads White PP Tray

SWA1168 PK100
EUR 166.44

Protect Beads Blue PP Tray

SWA1170 PK100
EUR 166.44

Protect Beads Yellow Bulk Pack

SWA1174 PK1000
EUR 956.46

Protect Beads Green Bulk Pack

SWA1176 PK1000
EUR 956.46

Protect Beads Red Bulk Pack

SWA1178 PK1000
EUR 956.46

Protect Beads White Bulk Pack

SWA1180 PK1000
EUR 956.46

Protect Beads Blue Bulk Pack

SWA1182 PK1000
EUR 956.46

VEGF-D Recombinant Protein

91-450 0.05 mg
EUR 651.3
Description: Vascular endothelial growth factor D (VEGF-D) is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It is highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. VEGF-D is growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. It may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. It undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3 receptors.

HLADG Recombinant Protein

92-450 0.05 mg
EUR 588.3
Description: Mouse HLA class II histocompatibility antigen gamma chain (CD74), is a single-pass type II membrane protein that in humans is encoded by the CD74 gene. It contains 1 thyroglobulin type-1 domain. CD74 Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

Her2 / ErbB2 Recombinant Protein

11-450 0.1 mg
EUR 651.3
Description: Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

CSF3 Recombinant Protein

90-450 10 ug
EUR 437.1
Description: G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage in vitro. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Filter for Accuris Microplate Reader

MR9600-450 1 PC
EUR 241.56

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Recombinant (CHO cells) Hepatitis B Surface Antigen adr subtype (HBsAg)

RP-450 20 ug
EUR 196.8

Benzo(j)fluoranthene

S-450 1ML
EUR 70.68

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 181.2

Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b)

MCD004-ML 100 ug
EUR 489.6

Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1)

MCD025-ML 100 ug
EUR 489.6

Paritaprevir(ABT-450)

20-abx184297
  • EUR 1496.40
  • EUR 1095.60
  • 10 mg
  • 5 mg

illustra Ready-To-Go RT-PCR Beads 0.2 ml tubes with hinged caps 96 rxns

27925901 EACH
EUR 372.78

illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml tubes/plate 5 x 96 rxns

27955702 EACH
EUR 656.64

illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml hinged tubes with cap 96 rxns

27955901 EACH
EUR 148.2

cfPure® V2 Cell Free DNA Extraction Kit, 100 ml

K5011610-V2 1 kit
EUR 537.6

cfPure® V2 Cell Free DNA Extraction Kit, 250 ml

K5011625-V2 1 kit
EUR 897.6

Carboxyl Magnetic Particles

CM-025-10 10 mL
EUR 249.6
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-025-100H 100mL
EUR 1765.2
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-025-10H 10mL
EUR 237.6
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-05-10 10 mL
EUR 249.6
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-05-10H 10mL
EUR 249.6
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-08-10 10 mL
EUR 249.6
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-10-10 10 mL
EUR 254.4
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-10-100 100 mL
EUR 1618.8
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-100-10 10 mL
EUR 408
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-1000-10 10 mL
EUR 511.2
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-15-10 10 mL
EUR 254.4
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Carboxyl Magnetic Particles

CM-15-100 100 mL
EUR 1618.8
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Nonetheless, EPS and acid manufacturing had been each diminished in L. casei CP002616 pLC2WΔ-HT (mutant pressure cured by high-temperature warmth remedy technique), demonstrating a distinction between these 2 curing strategies. Sequence evaluation of pLC2W and plasmid curing information recommend that plasmid pLC2W shouldn’t be concerned in EPS synthesis.

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