Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to several types of stress. Genome enhancing of Pputida largely depends on homologous recombination occasions, assisted by helper plasmid-based expression of genes encoding DNA modifying enzymes. Plasmid curing from chosen isolates is probably the most tedious and time-consuming step of this process, and implementing generally used strategies to this finish in Pputida (e.g. temperature-sensitive replicons) is usually impractical.

To deal with this difficulty, we have now developed a toolbox for each target- and self-curing of plasmid DNA in Pseudomonas species. Our technique allows plasmid-curing in a easy cultivation step by combining in vivo digestion of vectors by the I-SceI homing nuclease with artificial management of plasmid replication, triggered by the addition of an inexpensive chemical inducer (3-methylbenzoate) to the medium. The system shows an effectivity of vector curing >90% and the screening of plasmid-free clones is drastically facilitated by means of fluorescent markers that may be chosen in accordance with the appliance supposed.

Moreover, fast genome engineering of Pputida utilizing self-curing plasmids is demonstrated via genome discount of the platform pressure EM42 by eliminating all genes encoding β-lactamases, the catabolic ben gene cluster, and the pyoverdine synthesis equipment. Physiological characterization of the ensuing streamlined pressure, Pputida SEM10, revealed advantageous options that could possibly be exploited for metabolic engineering.

The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.

For many years, various plasmid vectors have been repeatedly developed for molecular cloning of DNA fragment within the bacterial host cell Escherichia coli. Even with deliberate performances in vector preparation, the cloning approaches nonetheless face inevitable background colonies, or false optimistic clones, which may be arisen from intact or self-ligated plasmid molecules. To help in such drawback, two plasmids, pBS2ndd and pBS3ndd, which proof against ampicillin and kanamycin respectively, had been developed on this examine as extra advantageous cloning vector. The plasmids carry ndd, a deadly gene from bacteriophage T4 coding for nucleoid disruption protein that binds to the host chromosome and progressively kill the cell.

The lethal toxicity of Ndd inhibits host cells that receive intact or ndd-religated vector from rising, which leads to low background and dramatically reduces the trouble for choice of recombinants. Furthermore, their equivalent a number of cloning web site was designed to help varied cloning methods. Digestion of plasmids with XcmI permits for in vitro T/A ligation, whereas with EcoRV permits blunt-end ligation, with functionality of blue-white colony screening. In vivo homologous recombination cloning can be utilizable by amplification of insert fragments utilizing primers containing homology arms and transformation into succesful E. coli strains.

To reveal their benefits, the plasmids had been used to clone PCR product samples for DNA sequencing with low-background and versatile cloning methods. Such fast and cost-effective cloning procedures are additionally proposed right here. Lastly, the cloning for protein expression with blue-white choice was additionally potential utilizing egfp as a mannequin regulated by lac and T7 promoters on the plasmid or different build-in promoters with the insert.

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.

Building of a plasmid vector containing epidermal development issue receptor and C-Jun shRNA.

The target of this examine was to assemble a plasmid vector for EGFR-hm-1 and C-Junh-825 small interfering RNA (siRNA). EGFR-hm-1 and C-Jun-hm-825 oligonucleotide fragments had been synthesized and quick hairpin RNA (shRNA) had been amplified by PCR. Plasmids had been remoted from E. coli TOP10 bacterium by restriction enzyme digestion utilizing pst1 and BamH1 and oligonucleotide fragments had been cloned into the pSilencer plasmid containing the U6 promoter.

Recombinant clones had been generated by remodeling JM109 competent cells with plasmid vectors containing shRNA molecules. 58 base-paired EGFR-hm-1 and 59 base-paired C-Jun-hm-825 oligonucleotide fragments had been remoted. The fragments had been 100% homologous with human sequences out there on GenBank. The recombinant pSilencer1.zero vector containing a 58-bp EGFR-hm-1 and 59-bp C-Jun-hm-825 fragment was constructed. These vectors have the potential for use as remedy to fight pores and skin photoaging underneath UV publicity.

Characterization of a cryptic plasmid remoted from Lactobacillus casei CP002616 and development of shuttle vectors based mostly on its replicon.

The cryptic plasmid pLC2W was remoted from Lactobacillus casei CP002616. Nucleotide sequence evaluation revealed that four putative open studying frames (ORF) had been answerable for DNA replication. 4 Escherichia coli-Lactobacillus shuttle vectors had been constructed utilizing completely different lengths of the pLC2W replicon to establish the shortest practical replicon. The size of the pLC2W replicon didn’t have an effect on the soundness of the plasmids. Inexperienced fluorescent protein (GFP) as a reporter was expressed efficiently in a number of lactobacilli utilizing our constructed vectors.

The outcomes urged that the expression vectors pUE-F0GFP and pUE-F1GFP are potential molecular instruments for heterologous gene cloning and expression in lactobacilli. Furthermore, 2 plasmid-curing strategies had been used to remove pLC2W from L. casei. We detected no distinction between L. casei CP002616 and L. casei CP002616 pLC2WΔ-IC (mutant pressure cured by plasmid incompatibility technique) in manufacturing of exopolysaccharide (EPS) or acid.

Magnetic Beads DNA Extraction Kit (Food)

MF004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Food)

MF048 each
EUR 55

Magnetic Beads DNA Extraction Kit (Food)

MF096 each
EUR 99

Magnetic Beads DNA Extraction Kit (Blood)

MB004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Blood)

MB048 each
EUR 40

Magnetic Beads DNA Extraction Kit (Blood)

MB096 each
EUR 75

Magnetic Beads DNA Extraction Kit (Plant)

MP004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Plant)

MP048 each
EUR 55

Magnetic Beads DNA Extraction Kit (Plant)

MP096 each
EUR 99

Magnetic Beads for DNA Purification

M1502-5 5 ml
EUR 332.4

Magnetic Beads DNA Extraction Kit (Bacteria)

ME004 each
EUR 3

Magnetic Beads DNA Extraction Kit (Bacteria)

ME048 each
EUR 60

Magnetic Beads DNA Extraction Kit (Bacteria)

ME096 each
EUR 110

Magnetic Beads for DNA size selection

AG0103 5 ml, 60 ml Ask for price
Description: The Magnetic Beads are ideal for DNA purification, concentration and size selection, along with next-generation sequencing library preparation kits.

Magnetic Beads-Based Plant Genomic DNA Kit

abx460019-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Plant Genomic DNA Kit

abx460019-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Plant Genomic DNA Kit

abx460019-96tests 96 tests
EUR 437.5

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-96tests 96 tests
EUR 437.5

FFPE Tissue DNA Extraction Kit - Magnetic Beads

K5011450 1 kit
EUR 261

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Tissue Genomic DNA Kit

abx460016-96tests 96 tests
EUR 437.5

Magnetic Beads Soil Genomic DNA Extraction Kit

MS004 each
EUR 3.5

Magnetic Beads Soil Genomic DNA Extraction Kit

MS048 each
EUR 65

Magnetic Beads Soil Genomic DNA Extraction Kit

MS096 each
EUR 120

Magnetic Beads-Based Bacteria Genomic DNA Kit

abx460017-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Bacteria Genomic DNA Kit

abx460017-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Bacteria Genomic DNA Kit

abx460017-96tests 96 tests
EUR 437.5

Magnetic Beads Viral DNA/RNA Extraction Kit II

MV004 each
EUR 3

Magnetic Beads Viral DNA/RNA Extraction Kit II

MV048 each
EUR 72

Magnetic Beads Viral DNA/RNA Extraction Kit II

MV096 each
EUR 128

Magnetic Beads Viral DNA/RNA Extraction Kit II

MV480 each
EUR 590

Magnetic Beads-Based Buffy Coat Genomic DNA Kit

abx460015-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Buffy Coat Genomic DNA Kit

abx460015-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Buffy Coat Genomic DNA Kit

abx460015-96tests 96 tests
EUR 437.5

Magnetic Beads (RNA)

AG0104 5 ml, 60 ml Ask for price
Description: The Magnetic Beads (RNA)</strong> are automated for highly efficient RNA purification by specific adsorption.

Streptavidin Magnetic Beads

HY-K0208 5 mL
EUR 645.6

Streptavidin Magnetic beads

EA-IP-011M 1mL
EUR 241
Description: Primary Antibodies

Anti-HA magnetic beads

B26201 1 mL
EUR 579.6
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA magnetic beads

B26202 5 mL
EUR 1742.4
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

SILICA MAGNETIC BEADS 1L

L5011001 1 liter
EUR 7260

Anti-HA Magnetic Beads

HY-K0201 1 mL
EUR 334.8

Protein A Magnetic Beads

6507-1 each
EUR 314.4

Protein G Magnetic Beads

6517-1 each
EUR 314.4

Protein L Magnetic Beads

6537-1 each
EUR 352.8

Anti-Myc magnetic beads

B26301 1 mL
EUR 579.6
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-Myc magnetic beads

B26302 5 mL
EUR 1742.4
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Protein A Magnetic Beads

HY-K0203 1 mL
EUR 148.8

Protein G Magnetic Beads

HY-K0204 5 mL
EUR 445.2

Protein A Magnetic beads

EA-IP-015M 1mL
EUR 105
Description: Primary Antibodies

Protein G Magnetic beads

EA-IP-016M 1mL
EUR 105
Description: Primary Antibodies

Protein L Magnetic beads

EA-IP-017M 1mL
EUR 105
Description: Primary Antibodies

Anti-Flag magnetic beads

B26101 1 mL
EUR 579.6
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26102 5 mL
EUR 1742.4
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

SILICA MAGNETIC BEADS 10ML

L5011010 10 ml
EUR 73

Anti-Flag Magnetic Beads

HY-K0207 1 mL
EUR 570

BlackPearl® (Magnetic Beads)

20161006-1 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-2 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-3 - Ask for price

Magnetic Beads™ Streptavidin

SMB-B01 5mg
EUR 149.8
Description: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA). The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly.Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Concanavalin A Magnetic beads

EA-IP-014M 1mL
EUR 159
Description: Primary Antibodies

IP/CoIP Kit (Magnetic Beads)

EA-IP-K007M 50T
EUR 700
Description: Antibody Application Reagents

Protein A/G Magnetic Beads

6527-1 each
EUR 352.8

Protein A/G Magnetic Beads

HY-K0202 1 mL
EUR 157.2

Protein A/G Magnetic Beads

EA-IP-007M 1mL
EUR 80
Description: Primary Antibodies

Anti-c-Myc Magnetic Beads

HY-K0206 5 mL
EUR 1368

Anti-tGFP magnetic beads, 1ml

TA150039 1 ml Ask for price

Anti-tGFP magnetic beads, 5ml

TA150040 5 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150044 1 ml Ask for price

9E10, Anti-Myc Magnetic beads

TA150045 5 ml Ask for price

Magnetic Beads PCR Cleanup Kit

MC004 each
EUR 3

Magnetic Beads PCR Cleanup Kit

MC048 each
EUR 25

Magnetic Beads PCR Cleanup Kit

MC096 each
EUR 45

Anti-HA (Nanobody) Magnetic Beads

AE109 500μL
EUR 403

Anti-GFP (Nanobody) Magnetic Beads

AE079 500μL
EUR 312

Anti-Myc (Nanobody) Magnetic Beads

AE107 1000μL
EUR 213.2

Protein A/G/L Magnetic Beads

6547-1 each
EUR 405.6

Human CD33-coupled Magnetic Beads

MBC-K008 2mg
EUR 2707.1
Description: Human CD33 protein is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259(Accession # P20138-1(R69G)).

Human CD38-coupled Magnetic Beads

MBC-K010 2mg
EUR 2707.1
Description: Human CD38 protein is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300(Accession # P28907-1).

Human EGFR-coupled Magnetic Beads

MBE-K012 2mg
EUR 2707.1
Description: Human EGFR protein is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645(Accession # P00533-1).

Human PSMA-coupled Magnetic Beads

MBP-K007 2mg
EUR 3349.1
Description: Human PSMA protein is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750(Accession # Q04609-1).

Human ROR1-coupled Magnetic Beads

MBR-K009 2mg
EUR 2707.1
Description: Human ROR1 protein is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403(Accession # Q01973-1).

Human BCMA-coupled Magnetic Beads

MBS-K004 2mg
EUR 2707.1
Description: Human BCMA protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54(Accession # Q02223-1).

Human CD19-coupled Magnetic Beads

MBS-K005 2mg
EUR 4312.1
Description: Human CD19 protein is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291(Accession # P15391-1).

Human HER2-coupled Magnetic Beads

MBS-K006 500ug
EUR 2835.5
Description: Human HER2 protein is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652(Accession # P04626-1).

Human ACE2-coupled Magnetic Beads

MBS-K013 2mg
EUR 2707.1
Description: Human ACE2 protein is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740(Accession # Q9BYF1-1).

Human CD73-coupled Magnetic Beads

MBS-K022 2mg
EUR 3349.1
Description: Human CD73 Protein is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549(Accession # P21589-1).

Human CD47-coupled Magnetic Beads

MBS-K023 2mg
EUR 2707.1
Description: Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).

Human CD3E-coupled Magnetic Beads

MBS-K026 2mg
EUR 2707.1
Description: Human CD3E Protein is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126(Accession # P07766-1).

Human CD22-coupled Magnetic Beads

MBS-K052 2mg
EUR 4312.1
Description: Human CD22 Protein is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687(Accession # P20273-1).

Human DLL3-coupled Magnetic Beads

MBS-K053 2mg
EUR 2707.1
Description: Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

Human uPAR-coupled Magnetic Beads

MBS-K076 2mg
EUR 2707.1
Description: Tumors are the result of uncontrolled proliferation of cells in different organs. Tumor development is a multistage process, that includes the generation of primary tumors, separation of tumors from primary sites, degradation of extracellular matrix (ECM), and distant metastasis of tumors. A variety of genes play important roles in the development of tumors, including the cell surface receptor urokinase-type plasminogen activator receptor (uPAR). uPAR is highly expressed in a variety of tumor cells, and a variety of signals regulated by uPAR play significant roles in tumor cell proliferation and metastasis, tumor-related glycolysis, the tumor microenvironment and angiogenesis. Studies have found that some specific drugs and antibodies have unique inhibitory effects on uPAR.

DDDDK-Tag Antibody (Magnetic Beads)

abx005594-100l 100 µl
EUR 250

Amylose BlackPearl® (Magnetic Beads)

20161009-1 - Ask for price

Amylose BlackPearl® (Magnetic Beads)

20161009-2 - Ask for price

Human PCSK9-coupled Magnetic Beads

MBS-K054 2mg
EUR 2707.1
Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Human ASGR1-coupled Magnetic Beads

MBS-K055 2mg
EUR 2707.1
Description: ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.

Human ANGPT2-coupled Magnetic Beads

MBS-K035 2mg
EUR 2707.1
Description: Human ANGPT2 Protein is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496(Accession # O15123-1).

Human VEGF165-coupled Magnetic Beads

MBS-K036 2mg
EUR 2707.1
Description: Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).

Ni-NTA Magnetic Agarose Beads

20-512M1 2ml/Unit
EUR 116.39
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M2 5ml/Unit
EUR 263.6
Description: Suspension, 5% Magnetic Resin

Ni-NTA Magnetic Agarose Beads

20-512M3 10ml/Unit
EUR 477.13
Description: Suspension, 5% Magnetic Resin

Human EGFRvIII-coupled Magnetic Beads

MBS-K020 2mg
EUR 2707.1
Description: Human EGFRvIII Protein is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378(Accession # NP_001333870.1).

Human HGF R-coupled Magnetic Beads

MBS-K021 2mg
EUR 2707.1
Description: Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).

Streptavidin BlackPearl® (Magnetic Beads)

20161004-1 - Ask for price

Streptavidin BlackPearl® (Magnetic Beads)

20161004-2 - Ask for price

Human Mesothelin-coupled Magnetic Beads

MBS-K024 2mg
EUR 2707.1
Description: Human Mesothelin Protein is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580(Accession # Q13421-3(R309P)).

Human CTLA-4 coupled Magnetic Beads

MBS-K042 2mg
EUR 2707.1
Description: Human CTLA-4 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162(Accession # Q6GR94-1).

Protein A/G Magnetic Beads for IP

B23201 1 ml
EUR 158.4
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A/G Magnetic Beads for IP

B23202 5 ml
EUR 558
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A BlackPearl® (Magnetic Beads)

20161000-1 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-2 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-3 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-1 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-2 - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-1 - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-2 - Ask for price

Human TROP-2-coupled Magnetic Beads

MBS-K028 2mg
EUR 2707.1
Description: Human TROP-2 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274(Accession # P09758-1).

Magnetic Beads Micro gDNA Extraction Kit

MM004 each
EUR 3

Magnetic Beads Micro gDNA Extraction Kit

MM048 each
EUR 63

Magnetic Beads Micro gDNA Extraction Kit

MM096 each
EUR 113

Anti-mCherry (Nanobody) Magnetic Beads Ab

AE094 1000μL
EUR 213.2

Human Nectin-4-coupled Magnetic Beads

MBS-K039 2mg
EUR 2707.1
Description: Human Nectin-4 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349(Accession # Q96NY8-1).

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-1 - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-2 - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-3 - Ask for price

Human B7-H3(4Ig)-coupled Magnetic Beads

MBS-K040 2mg
EUR 2707.1
Description: Human B7-H3 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461(Accession # Q5ZPR3-1).

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-1 - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-2 - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-3 - Ask for price

Mouse monoclonal eRFP antibody, Magnetic beads

TA183034 1 ml Ask for price

Mouse monoclonal eCFP antibody, Magnetic beads

TA183036 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183041 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183042 1 ml Ask for price

Mouse monoclonal mRFP antibody, Magnetic beads

TA183043 1 ml Ask for price

Mouse monoclonal mPlum antibody, Magnetic beads

TA183037 1 ml Ask for price

Clone OTI4C5, Anti-DDK (FLAG) Magnetic beads

TA150036 5 ml Ask for price

Clone OTI4C5, Anti-DDK (FLAG) Magnetic beads

TA150042 1 ml Ask for price

Universal IP/Co-IP Toolkit (Magnetic Beads)

KTD104-EN-20T 20 T
EUR 169
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

Mouse monoclonal DsRed2 antibody, Magnetic beads

TA183025 1 ml Ask for price

Mouse monoclonal AsRed2 antibody, Magnetic beads

TA183045 1 ml Ask for price

Mouse monoclonal mCherry antibody, Magnetic beads

TA183030 1 ml Ask for price

Mouse monoclonal mBanana antibody, Magnetic beads

TA183032 1 ml Ask for price

Mouse monoclonal Dendra2 antibody, Magnetic beads

TA183044 1 ml Ask for price

Mouse monoclonal tdTomato antibody, Magnetic beads

TA183027 1 ml Ask for price

Magnetic Beads Virus DNA/RNA Extraction Plate Kit (6 plates, 16 tests/plate, 96 rxns)

MVP096 each
EUR 132.5

Mouse monoclonal ZsYellow1 antibody, Magnetic beads

TA183024 1 ml Ask for price

PE-labeled Human CD47-coupled Magnetic Beads

MBS-K059 2.5mg
EUR 4536.8
Description: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Mouse monoclonal mStrawberry antibody, Magnetic beads

TA183033 1 ml Ask for price

APC-labeled Human CD47-coupled Magnetic Beads

MBS-K060 0.5mg
EUR 3028.1
Description: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

SARS-CoV-2 Spike S1-coupled Magnetic Beads

MBS-K001 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike S1 protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685(Accession # QHD43416.1).

SARS-CoV-2 Spike S2-coupled Magnetic Beads

MBS-K018 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike S2 Protein is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1 (F817P, A892P, A899P, A942P,K986P,V987P)).

SARS-CoV-2 Spike RBD-coupled Magnetic Beads

MBS-K002 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541(Accession # QHD43416.1).

SARS-CoV-2 Spike NTD-coupled Magnetic Beads

MBS-K019 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike NTD Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Phe 318(Accession # QHD43416.1).

Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

MBS-K003 2mg
EUR 2707.1
Description: Human CD3E & CD3D Heterodimer protein is expressed from human 293 cells (HEK293).It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).

Mouse monoclonal E2-Crimson antibody, Magnetic beads

TA183040 1 ml Ask for price

Magnetic Beads-conjugated Rabbit IgG isotype control

AC047 100μL
EUR 87.75
Description: None

SARS-CoV-2 Spike Trimer-coupled Magnetic Beads

MBS-K015 2mg
EUR 4312.1
Description: SARS-CoV-2 Spike Trimer protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (R683A, R685A)).

Mouse monoclonal DsRed-Monomer antibody, Magnetic beads

TA183039 1 ml Ask for price

Magnetic Beads-Based Virus Genomic Nucleic Acid Kit

abx460018-1096tests 10 × 96 tests Ask for price

Magnetic Beads-Based Virus Genomic Nucleic Acid Kit

abx460018-596tests 5 × 96 tests Ask for price

Magnetic Beads-Based Virus Genomic Nucleic Acid Kit

abx460018-96tests 96 tests
EUR 437.5

Mouse monoclonal DsRed-Express2 antibody, Magnetic beads

TA183029 1 ml Ask for price

Mouse monoclonal mOrange/mOrange2 antibody, Magnetic beads

TA183035 1 ml Ask for price

Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb

AE037 50 ul
EUR 357.5

B2M mouse monoclonal antibody,clone 1A9, Magnetic Beads

TA802776JM 1 ml Ask for price

Magnetic Beads-conjugated Rabbit anti DDDDK-Tag mAb

AE097 1000μL
EUR 84.5

HA mouse monoclonal antibody, clone CB051, Magnetic Beads

TA183070 1 ml Ask for price

Mouse monoclonal mRaspberry/AmCyan1 antibody, Magnetic beads

TA183031 1 ml Ask for price

Protein A-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A001 10mg
EUR 866.7
Description: The Protein A-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein A. The beads can be used to capture the antibodies in Chemiluminescence procedures.The Protein A is a 42 kDa surface protein, It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family.The Anti-Protein A-coupled Magnetic Beads is easy to capture the antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Protein G-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A002 10mg
EUR 866.7
Description: The Protein G-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein G. The beads can be used to capture the antibodies in Chemiluminescence procedures.The Protein G binds the heavy chain within the Fc region of most immunoglobulins but not bind dog immunoglobulins.The Protein G-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Protein L-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A008 10mg
EUR 866.7
Description: The Protein L-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of Protein L. The beads can be used to capture the antibodies in Chemiluminescence procedures. The Protein L is a 45 kDa surface protein, and can bind almost all antibody types, including IgG, IgM, IgA, IgE and IgD. It binds to antibodies with appropriate Kappa light chains such as VkI, VkIII and VkIV, whereas no binding occurred with antibodies of the VkII or with any Lambda light chains. The Protein L-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Mouse monoclonal AcGFP1/PS-CFP2 antibody, Magnetic beads

TA183028 1 ml Ask for price

resDetect™ resDNA Sample Preparation Kit (Magnetic Beads)

OPA-R005 50preps
EUR 310.3
Description: DNA extraction is a critical step in many molecular biology techniques, such as PCR, sequencing, and cloning, as it is necessary to obtain pure and intact DNA that can be used in downstream applications. DNA extraction kits can simplify and standardize the DNA extraction process, allowing researchers to obtain high-quality DNA from a wide range of samples with reproducible results.

SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads

MBS-K032 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (B.1.351) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (K417N,E484K,N501Y)).

SARS-CoV-2 Spike RBD (P.1) Coupled Magnetic Beads

MBS-K033 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (P.1) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (K417T,E484K,N501Y)).

SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads

MBS-K034 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1(N501Y)).

SARS-CoV-2 (Delta) Spike RBD-coupled Magnetic Beads

MBS-K037 2mg
EUR 2707.1
Description: SARS-CoV-2 (Delta) Spike RBD Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (L452R, T478K)).

SARS-CoV-2 Spike Trimer (D614G)-coupled Magnetic Beads

MBS-K016 2mg
EUR 4312.1
Description: SARS-CoV-2 Spike Trimer (D614G) protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (D614G, R683A, R685A)).

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)

KTI1010-EN-20T 20 T
EUR 139
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

Mouse monoclonal Timer antibody, clone OTI3C9, Magnetic beads

TA183026 1 ml Ask for price

SARS-CoV-2 (Omicron) Spike RBD-coupled Magnetic Beads

MBS-K043 2mg
EUR 2707.1
Description: SARS-CoV-2 (Omicron) Spike RBD Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H)).

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)

KTI1020-EN-20T 20 T
EUR 139
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

SARS-CoV-2 Nucleocapsid Protein-coupled Magnetic Beads

MBS-K017 2mg
EUR 2707.1
Description: SARS-CoV-2 Nucleocapsid Protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419(Accession # QHO62115.1).

SARS-CoV-2 Spike Trimer (B.1.1.7) Coupled Magnetic Beads

MBS-K029 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike Trimer (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (HV69-70del/Y144del//N501Y/A570D/D614G/P681H/T716I/S982A/D1118H/R683A/R685A/F817P/A892P/A899P/A942P/K986P/V987P)).

SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads

MBS-K030 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike Trimer (B.1.351) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V, R683A, R685A,F817P, A892P, A899P, A942P, K986P, V987P)).

SARS-CoV-2 Spike Trimer (P.1) Coupled Magnetic Beads

MBS-K031 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike Trimer (P.1) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F, R683A, R685A,F817P, A892P, A899P, A942P, K986P, V987P)).

SARS-CoV-2 (Delta) Spike Trimer-coupled Magnetic Beads

MBS-K038 2mg
EUR 4312.1
Description: SARS-CoV-2 (Delta) Spike Trimer Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)).

Mouse monoclonal ZsGreen1 antibody, clone OTI2C2, Magnetic beads

TA183023 1 ml Ask for price

Anti-Mouse IgG-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A003 10mg
EUR 1305.4
Description: The Anti-Mouse IgG-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity polyclonal goat anti-mouse IgG antibody. The beads can be used to capture the mouse IgG in Chemiluminescence procedures.The antibody reacts with the Fc or Fab portion of mouse IgG. No antibody was detected against mouse IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.The Anti-Mouse IgG-coupled Magnetic Beads is easy to capture the mouse IgG, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A004 10mg
EUR 984.4
Description: The Anti-Human IgG-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal mouse anti-human IgG antibody. The beads can be used to capture the human IgG in Chemiluminescence procedures.The antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, cynomolgus and bovine serum proteins, but it is not sure if the product cross-react with immunoglobulins from other species.The Anti-Human IgG-coupled Magnetic Beads is easy to capture the human IgG, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A010 100mg
EUR 107
Description: The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, Magnetic beads

TA183038 1 ml Ask for price

DiagNano™ PEG Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ012 150 ul
EUR 960

DiagNano™ CD4 Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ013 150 ul
EUR 960

DiagNano™ PEG Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ015 150 ul
EUR 960

DiagNano™ CD3 Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ001 20 tests
EUR 1160

DiagNano™ CD4 Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ002 20 tests
EUR 1160

Nonetheless, EPS and acid manufacturing had been each diminished in L. casei CP002616 pLC2WΔ-HT (mutant pressure cured by high-temperature warmth remedy technique), demonstrating a distinction between these 2 curing strategies. Sequence evaluation of pLC2W and plasmid curing information recommend that plasmid pLC2W shouldn’t be concerned in EPS synthesis.

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