Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and excessive ranges of tolerance to several types of stress. Genome enhancing of P. putida largely depends on homologous recombination occasions, assisted by helper plasmid-based expression of genes encoding DNA modifying enzymes. Plasmid curing from chosen isolates is probably the most tedious and time-consuming step of this process, and implementing generally used strategies to this finish in P. putida (e.g. temperature-sensitive replicons) is usually impractical.
To deal with this difficulty, we have now developed a toolbox for each target- and self-curing of plasmid DNA in Pseudomonas species. Our technique allows plasmid-curing in a easy cultivation step by combining in vivo digestion of vectors by the I-SceI homing nuclease with artificial management of plasmid replication, triggered by the addition of an inexpensive chemical inducer (3-methylbenzoate) to the medium. The system shows an effectivity of vector curing >90% and the screening of plasmid-free clones is drastically facilitated by means of fluorescent markers that may be chosen in accordance with the appliance supposed.
Moreover, fast genome engineering of P. putida utilizing self-curing plasmids is demonstrated via genome discount of the platform pressure EM42 by eliminating all genes encoding β-lactamases, the catabolic ben gene cluster, and the pyoverdine synthesis equipment. Physiological characterization of the ensuing streamlined pressure, P. putida SEM10, revealed advantageous options that could possibly be exploited for metabolic engineering.
The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.
For many years, various plasmid vectors have been repeatedly developed for molecular cloning of DNA fragment within the bacterial host cell Escherichia coli. Even with deliberate performances in vector preparation, the cloning approaches nonetheless face inevitable background colonies, or false optimistic clones, which may be arisen from intact or self-ligated plasmid molecules. To help in such drawback, two plasmids, pBS2ndd and pBS3ndd, which proof against ampicillin and kanamycin respectively, had been developed on this examine as extra advantageous cloning vector. The plasmids carry ndd, a deadly gene from bacteriophage T4 coding for nucleoid disruption protein that binds to the host chromosome and progressively kill the cell.
The lethal toxicity of Ndd inhibits host cells that receive intact or ndd-religated vector from rising, which leads to low background and dramatically reduces the trouble for choice of recombinants. Furthermore, their equivalent a number of cloning web site was designed to help varied cloning methods. Digestion of plasmids with XcmI permits for in vitro T/A ligation, whereas with EcoRV permits blunt-end ligation, with functionality of blue-white colony screening. In vivo homologous recombination cloning can be utilizable by amplification of insert fragments utilizing primers containing homology arms and transformation into succesful E. coli strains.
To reveal their benefits, the plasmids had been used to clone PCR product samples for DNA sequencing with low-background and versatile cloning methods. Such fast and cost-effective cloning procedures are additionally proposed right here. Lastly, the cloning for protein expression with blue-white choice was additionally potential utilizing egfp as a mannequin regulated by lac and T7 promoters on the plasmid or different build-in promoters with the insert.
Building of a plasmid vector containing epidermal development issue receptor and C-Jun shRNA.
The target of this examine was to assemble a plasmid vector for EGFR-hm-1 and C-Junh-825 small interfering RNA (siRNA). EGFR-hm-1 and C-Jun-hm-825 oligonucleotide fragments had been synthesized and quick hairpin RNA (shRNA) had been amplified by PCR. Plasmids had been remoted from E. coli TOP10 bacterium by restriction enzyme digestion utilizing pst1 and BamH1 and oligonucleotide fragments had been cloned into the pSilencer plasmid containing the U6 promoter.
Recombinant clones had been generated by remodeling JM109 competent cells with plasmid vectors containing shRNA molecules. 58 base-paired EGFR-hm-1 and 59 base-paired C-Jun-hm-825 oligonucleotide fragments had been remoted. The fragments had been 100% homologous with human sequences out there on GenBank. The recombinant pSilencer1.zero vector containing a 58-bp EGFR-hm-1 and 59-bp C-Jun-hm-825 fragment was constructed. These vectors have the potential for use as remedy to fight pores and skin photoaging underneath UV publicity.
Characterization of a cryptic plasmid remoted from Lactobacillus casei CP002616 and development of shuttle vectors based mostly on its replicon.
The cryptic plasmid pLC2W was remoted from Lactobacillus casei CP002616. Nucleotide sequence evaluation revealed that four putative open studying frames (ORF) had been answerable for DNA replication. 4 Escherichia coli-Lactobacillus shuttle vectors had been constructed utilizing completely different lengths of the pLC2W replicon to establish the shortest practical replicon. The size of the pLC2W replicon didn’t have an effect on the soundness of the plasmids. Inexperienced fluorescent protein (GFP) as a reporter was expressed efficiently in a number of lactobacilli utilizing our constructed vectors.
The outcomes urged that the expression vectors pUE-F0GFP and pUE-F1GFP are potential molecular instruments for heterologous gene cloning and expression in lactobacilli. Furthermore, 2 plasmid-curing strategies had been used to remove pLC2W from L. casei. We detected no distinction between L. casei CP002616 and L. casei CP002616 pLC2WΔ-IC (mutant pressure cured by plasmid incompatibility technique) in manufacturing of exopolysaccharide (EPS) or acid.
 Anti-Myc magnetic beads |
|
B26301 |
Bimake |
1 mL |
EUR 579.6 |
|
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
Anti-Myc magnetic beads |
|
B26302 |
Bimake |
5 mL |
EUR 1742.4 |
|
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
 Protein A Magnetic Beads |
|
6507-1 |
Biovision |
each |
EUR 314.4 |
 Protein G Magnetic Beads |
|
6517-1 |
Biovision |
each |
EUR 314.4 |
 Protein L Magnetic Beads |
|
6537-1 |
Biovision |
each |
EUR 352.8 |
 Protein A/G Magnetic Beads |
|
6527-1 |
Biovision |
each |
EUR 352.8 |
 Size Selection DNA Beads |
|
20-abx298001 |
Abbexa |
-
EUR 260.40
-
EUR 477.60
-
EUR 1663.20
|
|
|
|
 VAHTS DNA Clean Beads |
|
N411-01 |
Vazyme |
5 ml |
EUR 259.2 |
VAHTS DNA Clean Beads |
|
N411-02 |
Vazyme |
60 ml |
EUR 634.8 |
VAHTS DNA Clean Beads |
|
N411-03 |
Vazyme |
450 ml |
EUR 3055.2 |
 Protein A/G/L Magnetic Beads |
|
6547-1 |
Biovision |
each |
EUR 405.6 |
 Protein A/G Magnetic Beads for IP |
|
B23201 |
Bimake |
1 ml |
EUR 158.4 |
|
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
Protein A/G Magnetic Beads for IP |
|
B23202 |
Bimake |
5 ml |
EUR 558 |
|
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
) Protein A Beads (1-2mg/ml) |
|
B0001-10 |
BosterBio |
10ml |
EUR 486 |
Protein A Beads (1-2mg/ml) |
|
B0001-25 |
BosterBio |
25ml |
EUR 973.2 |
Protein A Beads (1-2mg/ml) |
|
B0001-5 |
BosterBio |
5ml |
EUR 291.6 |
) Protein G Beads (1-2mg/ml) |
|
B0002-10 |
BosterBio |
10ml |
EUR 729.6 |
Protein G Beads (1-2mg/ml) |
|
B0002-25 |
BosterBio |
25ml |
EUR 1472.4 |
Protein G Beads (1-2mg/ml) |
|
B0002-5 |
BosterBio |
5ml |
EUR 426 |
 100 BP DNA LADDER, 500UL PER KIT |
|
M-DNA-100BP |
CORNING |
1/pk |
EUR 87.6 |
|
Description: Bioscience Mol Bio; DNA Ladder |
 1 KB DNA LADDER, 500UL PER KIT |
|
M-DNA-1KB |
CORNING |
1/pk |
EUR 84 |
|
Description: Bioscience Mol Bio; DNA Ladder |
 Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb |
|
AE037 |
Abclonal |
50 ul |
EUR 211.2 |
) Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube) |
|
M-CO-501 |
MiTeGen |
24 glass beads |
EUR 71 |
|
|
|
Description: Beads-for-Seeds (24 glass beads, each in a 1.5 ml tube) |
) Goat Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0003-10 |
BosterBio |
10ml |
EUR 681.6 |
Goat Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0003-25 |
BosterBio |
25ml |
EUR 1228.8 |
Goat Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0003-5 |
BosterBio |
5ml |
EUR 426 |
) Goat Anti-Mouse IgG Beads (1-1.5mg/ml) |
|
B0004-10 |
BosterBio |
10ml |
EUR 644.4 |
Goat Anti-Mouse IgG Beads (1-1.5mg/ml) |
|
B0004-25 |
BosterBio |
25ml |
EUR 1228.8 |
Goat Anti-Mouse IgG Beads (1-1.5mg/ml) |
|
B0004-5 |
BosterBio |
5ml |
EUR 400.8 |
) Rabbit Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0005-10 |
BosterBio |
10ml |
EUR 949.2 |
Rabbit Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0005-25 |
BosterBio |
25ml |
EUR 2008.8 |
Rabbit Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0005-5 |
BosterBio |
5ml |
EUR 547.2 |
) Rabbit Anti-Rat IgG Beads (1-1.5mg/ml) |
|
B0006-10 |
BosterBio |
10ml |
EUR 949.2 |
Rabbit Anti-Rat IgG Beads (1-1.5mg/ml) |
|
B0006-25 |
BosterBio |
25ml |
EUR 2008.8 |
Rabbit Anti-Rat IgG Beads (1-1.5mg/ml) |
|
B0006-5 |
BosterBio |
5ml |
EUR 547.2 |
) Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0007-10 |
BosterBio |
10ml |
EUR 644.4 |
Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0007-25 |
BosterBio |
25ml |
EUR 1228.8 |
Mouse Anti-Rabbit IgG Beads (1-1.5mg/ml) |
|
B0007-5 |
BosterBio |
5ml |
EUR 400.8 |
) Mouse Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0008-10 |
BosterBio |
10ml |
EUR 949.2 |
Mouse Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0008-25 |
BosterBio |
25ml |
EUR 2008.8 |
Mouse Anti-Human IgG Beads (1-1.5mg/ml) |
|
B0008-5 |
BosterBio |
5ml |
EUR 547.2 |
) RNA/DNA Purification Kit (Magnetic Bead) |
|
DA0623 |
Daan Gene |
32 test/kit |
Ask for price |
) Circulating DNA Kit (with Magnetic Stand) |
|
abx098869-50rxns |
Abbexa |
50 rxns |
EUR 510 |
|
|
Circulating DNA Kit (with Magnetic Stand) |
|
abx298026-50rxns |
Abbexa |
50 rxns |
EUR 510 |
|
|
 Cyanine7 tetrazine, 25 mg |
|
450 |
Lumiprobe |
25 mg |
EUR 615.6 |
) Blood Genomic DNA Kit (with Magnetic Stand) |
|
abx098867-50rxns |
Abbexa |
50 rxns |
EUR 360 |
|
|
) Viral DNA / RNA Kit (with Magnetic Stand) |
|
abx098871-50rxns |
Abbexa |
50 rxns |
EUR 477.6 |
|
|
) Viral DNA/RNA Kit (with Magnetic Stand) |
|
abx298029-50rxns |
Abbexa |
50 rxns |
EUR 477.6 |
|
|
 Exorose Beads |
|
EXOB-100 |
ABTBeads |
100 ml |
EUR 127.2 |
Exorose Beads |
|
EXOB-25 |
ABTBeads |
25 ml |
EUR 69.6 |
Exorose Beads |
|
EXOB-250 |
ABTBeads |
250 ml |
EUR 225.6 |
 Latex Beads |
|
abx291003-1L |
Abbexa |
1 L |
EUR 2798.4 |
|
|
 RNA Beads |
|
20-abx298002 |
Abbexa |
-
EUR 260.40
-
EUR 477.60
-
EUR 1663.20
|
|
|
|
) T4 DNA Polymerase (5,000 U/ml) |
|
RK20539 |
Abclonal |
25 U |
Ask for price |
|
|
 V5 agarose beads |
|
AE029-100ul |
Abclonal |
100 ul |
Ask for price |
V5 agarose beads |
|
AE029-200ul |
Abclonal |
200 ul |
EUR 273.6 |
V5 agarose beads |
|
AE029-20ul |
Abclonal |
20 ul |
Ask for price |
V5 agarose beads |
|
AE029-50ul |
Abclonal |
50 ul |
Ask for price |
 Glass Beads 0.1mm |
|
GB01 |
Next Advance |
1pack |
EUR 129.6 |
|
Description: Glass beads. 0.1mm. 1 lb. Non-sterile. |
 Glass Beads 0.5mm |
|
GB05 |
Next Advance |
1pack |
EUR 123.6 |
|
Description: Glass beads. 0.5mm. 1 lb. Non-sterile. |
 Glass Beads 1.0mm |
|
GB10 |
Next Advance |
1pack |
EUR 123.6 |
|
Description: Glass beads. 1.0mm. 1 lb. Non-sterile. |
 Sodium hydroxide, Beads |
|
SB6789 |
Bio Basic |
500g |
EUR 73.21 |
|
|
 RanBP1 Agarose Beads |
|
STA-421 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Streptavidin-Sepharose Beads |
|
6565-10 |
Biovision |
each |
EUR 836.4 |
Streptavidin-Sepharose Beads |
|
6565-100 |
Biovision |
each |
Ask for price |
Streptavidin-Sepharose Beads |
|
6565-2 |
Biovision |
each |
EUR 274.8 |
Streptavidin-Sepharose Beads |
|
6565-5 |
Biovision |
each |
EUR 483.6 |
 EZEnrich? Polyubiquitin Beads |
|
6568-300 |
Biovision |
each |
EUR 444 |
 Thrombin Sepharose Beads |
|
7925-1 |
Biovision |
each |
EUR 261.6 |
Thrombin Sepharose Beads |
|
7925-25 |
Biovision |
each |
EUR 2114.4 |
Thrombin Sepharose Beads |
|
7925-5 |
Biovision |
each |
EUR 836.4 |
 Plasmin Sepharose Beads |
|
7926-1 |
Biovision |
each |
EUR 261.6 |
Plasmin Sepharose Beads |
|
7926-25 |
Biovision |
each |
EUR 2114.4 |
Plasmin Sepharose Beads |
|
7926-5 |
Biovision |
each |
EUR 836.4 |
 Urokinase Sepharose Beads |
|
7927-1 |
Biovision |
each |
EUR 261.6 |
Urokinase Sepharose Beads |
|
7927-25 |
Biovision |
each |
EUR 2114.4 |
Urokinase Sepharose Beads |
|
7927-5 |
Biovision |
each |
EUR 836.4 |
 Immobilized Catalase Beads |
|
7931-1 |
Biovision |
each |
EUR 183.6 |
Immobilized Catalase Beads |
|
7931-10 |
Biovision |
each |
EUR 705.6 |
 Calmodulin-Sepharose Beads |
|
7934-1 |
Biovision |
each |
EUR 183.6 |
Calmodulin-Sepharose Beads |
|
7934-10 |
Biovision |
each |
EUR 574.8 |
Calmodulin-Sepharose Beads |
|
7934-5 |
Biovision |
each |
EUR 405.6 |
) Blood Genomic DNA Kit (0.1-20 ml) |
|
abx098074-For200mlBlood |
Abbexa |
For 200 ml Blood |
EUR 427.2 |
|
|
Blood Genomic DNA Kit (0.1-20 ml) |
|
abx098074-For50mlBlood |
Abbexa |
For 50 ml Blood |
EUR 260.4 |
|
|
 Cot I human DNA conc. >=1,1 mg / ml |
|
3001 |
GeneOn |
500 µg |
EUR 140.4 |
 VAHTS mRNA Capture Beads |
|
N401-01 |
Vazyme |
24 rxn |
EUR 171.6 |
VAHTS mRNA Capture Beads |
|
N401-02 |
Vazyme |
96 rxn |
EUR 306 |
 VAHTS RNA Clean Beads |
|
N412-01 |
Vazyme |
5 ml |
EUR 286.8 |
VAHTS RNA Clean Beads |
|
N412-02 |
Vazyme |
40 ml |
EUR 495.6 |
VAHTS RNA Clean Beads |
|
N412-03 |
Vazyme |
450 ml |
EUR 4860 |
 Stainless Steel Beads 0.2mm |
|
SSB02 |
Next Advance |
1pack |
EUR 229.2 |
|
Description: Stainless steel beads. 0.2mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 0.5mm |
|
SSB05 |
Next Advance |
1pack |
EUR 229.2 |
|
Description: Stainless steel beads. 0.5mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 1.6mm |
|
SSB16 |
Next Advance |
1pack |
EUR 304.8 |
|
Description: Stainless steel beads. 1.6mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 2.3mm |
|
SSB23 |
Next Advance |
1pack |
EUR 260.4 |
|
Description: Stainless steel beads. 2.3mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 3.2mm |
|
SSB32 |
Next Advance |
1pack |
EUR 220.8 |
|
Description: Stainless steel beads. 3.2mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 4.8mm |
|
SSB48 |
Next Advance |
1pack |
EUR 220.8 |
|
Description: Stainless steel beads. 4.8mm. 1 lb. Non-sterile. |
 Stainless Steel Beads 6.0mm |
|
SSB60 |
Next Advance |
1pack |
EUR 223.2 |
|
Description: Stainless steel beads. 6.0mm. 1 lb. Non-sterile. |
 PAK1 PBD Agarose Beads |
|
STA-411 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
|
Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Rhotekin RBD Agarose Beads |
|
STA-412 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
|
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 RalGDS RBD Agarose Beads |
|
STA-418 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 GGA3 PBD Agarose Beads |
|
STA-419 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 RalBP1 PBD Agarose Beads |
|
STA-420 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Zirconium Oxide Beads 0.15mm |
|
ZrOB015 |
Next Advance |
1pack |
EUR 189.6 |
|
Description: Fused Zirconium Oxide beads . 0.15mm. 1 lb. Non-sterile. |
 Zirconium Oxide Beads 0.5mm |
|
ZrOB05 |
Next Advance |
1pack |
EUR 189.6 |
|
Description: Zirconium Oxide beads . 0.5mm. 1 lb. Non-sterile. |
 Zirconium Oxide Beads 1.0mm |
|
ZrOB10 |
Next Advance |
1pack |
EUR 189.6 |
|
Description: Zirconium Oxide beads . 1.0mm. 1 lb. Non-sterile. |
 Zirconium Oxide Beads 2.0mm |
|
ZrOB20 |
Next Advance |
1pack |
EUR 189.6 |
|
Description: Zirconium Oxide beads . 2.0mm. 1 lb. Non-sterile. |
 Zirconium Oxide Beads 2.5mm |
|
ZrOB25 |
Next Advance |
1pack |
EUR 189.6 |
|
Description: Zirconium Oxide beads . 2.5mm. 1 lb. Non-sterile. |
 Zirconium Silicate Beads 0.5mm |
|
ZSB05 |
Next Advance |
1pack |
EUR 146.4 |
|
Description: Zirconium Silicate beads . 0.5mm. 1 lb. Non-sterile. |
 Zirconium Silicate Beads 1.0mm |
|
ZSB10 |
Next Advance |
1pack |
EUR 146.4 |
|
Description: Zirconium Silicate beads . 1.0mm. 1 lb. Non-sterile. |
 Calibration Beads for MoFlo |
|
B28479 |
Spherotech |
each |
EUR 346.8 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
 Magnetic Stirrer |
|
abx725038-1Unit |
Abbexa |
1 Unit |
EUR 518.4 |
|
|
 Magnetic Stand |
|
abx799001-1Unit |
Abbexa |
1 Unit |
EUR 309.6 |
|
|
 Magnetic Separator |
|
B23803 |
Bimake |
2 mL/200 uL/15 mL |
EUR 291.6 |
|
Description: This Magnetic Separator is a proprietary machine, supportinga the magnetic beads series of products. A core tenent is the use of a superior magnetic, to ensure that magnetic separation step can be completed in a short time. |
Magnetic Separator |
|
MagSep1-1 |
ABTBeads |
1/pk |
EUR 132 |
) RNase-Free Calf Thymus DNA, 1 mg/mL (5x1mL) |
|
9-31065 |
Biotium |
|
|
|
Description: Minimum order quantity: 1 unit of 5tubes |
 cfPure® Cell Free DNA Extraction Kit, 100 ml |
|
K5011610 |
Biochain |
1 kit |
EUR 537.6 |
 cfPure® Cell Free DNA Extraction Kit, 250 ml |
|
K5011625 |
Biochain |
1 kit |
EUR 897.6 |
 VEGF-D Recombinant Protein |
|
91-450 |
ProSci |
0.05 mg |
EUR 651.3 |
|
Description: Vascular endothelial growth factor D (VEGF-D) is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It is highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. VEGF-D is growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. It may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. It undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3 receptors. |
 HLADG Recombinant Protein |
|
92-450 |
ProSci |
0.05 mg |
EUR 588.3 |
|
Description: Mouse HLA class II histocompatibility antigen gamma chain (CD74), is a single-pass type II membrane protein that in humans is encoded by the CD74 gene. It contains 1 thyroglobulin type-1 domain. CD74 Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. |
 Her2 / ErbB2 Recombinant Protein |
|
11-450 |
ProSci |
0.1 mg |
EUR 651.3 |
|
Description: Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation . |
 CSF3 Recombinant Protein |
|
90-450 |
ProSci |
10 ug |
EUR 437.1 |
|
Description: G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage in vitro. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. |
 GFP-Rac1 Expression Vector Set |
|
STA-450 |
Cell Biolabs |
1 kit |
EUR 1939.2 |
|
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence. |
 Hepatitis B Surface Antigen adr subtype (HBsAg)) Recombinant (CHO cells) Hepatitis B Surface Antigen adr subtype (HBsAg) |
|
RP-450 |
Alpha Diagnostics |
20 ug |
EUR 196.8 |
) Paritaprevir(ABT-450) |
|
20-abx184297 |
Abbexa |
|
|
|
|
 Glass Beads 0.1mm RNase Free |
|
GB01-RNA |
Next Advance |
1pack |
EUR 115.2 |
|
Description: Glass beads. 0.1mm. 4mL. RNase free. |
 Glass Beads 0.5mm RNase Free |
|
GB05-RNA |
Next Advance |
1pack |
EUR 115.2 |
|
Description: Glass beads. 0.5mm. 4mL. RNase free. |
 Stainless Steel UFO Beads 3.5mm |
|
SSUFO35 |
Next Advance |
1pack |
EUR 229.2 |
|
Description: Stainless steel UFO beads. 3.5mm. 50mL. Non-sterile. |
 Stainless Steel UFO Beads 5.6mm |
|
SSUFO56 |
Next Advance |
1pack |
EUR 225.6 |
|
Description: Stainless steel UFO beads. 5.6mm. 50mL. Non-sterile. |
 Raf-1 RBD Agarose Beads |
|
STA-410 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
|
Description: Raf-1 RBD Agarose Beads selectively pull down the active form of Ras. Beads are colored to allow for a visual check. These are the same beads supplied with our Ras Activation Assays. 400 µg. |
 Calibration Beads for MoFlo Astrios |
|
B27034 |
Spherotech |
each |
EUR 346.8 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
 cfPure® V2 Cell Free DNA Extraction Kit, 100 ml |
|
K5011610-V2 |
Biochain |
1 kit |
EUR 537.6 |
 cfPure® V2 Cell Free DNA Extraction Kit, 250 ml |
|
K5011625-V2 |
Biochain |
1 kit |
EUR 897.6 |
 BrightGreen miRNA qPCR MasterMix-Low ROX |
|
MasterMix-mL |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 181.2 |
 (rat IgG2b)) Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b) |
|
MCD004-ML |
Alpha Diagnostics |
100 ug |
EUR 489.6 |
 (rat IgG1)) Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1) |
|
MCD025-ML |
Alpha Diagnostics |
100 ug |
EUR 489.6 |
 Carboxyl Magnetic Particles |
|
CM-025-10 |
Spherotech |
10 mL |
EUR 249.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-025-100H |
Spherotech |
100mL |
EUR 1765.2 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-025-10H |
Spherotech |
10mL |
EUR 237.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-05-10 |
Spherotech |
10 mL |
EUR 249.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-05-10H |
Spherotech |
10mL |
EUR 249.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-08-10 |
Spherotech |
10 mL |
EUR 249.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-10-10 |
Spherotech |
10 mL |
EUR 254.4 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-10-100 |
Spherotech |
100 mL |
EUR 1618.8 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-100-10 |
Spherotech |
10 mL |
EUR 408 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-1000-10 |
Spherotech |
10 mL |
EUR 511.2 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-15-10 |
Spherotech |
10 mL |
EUR 254.4 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-15-100 |
Spherotech |
100 mL |
EUR 1618.8 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-150-10 |
Spherotech |
10 mL |
EUR 444 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-20-10 |
Spherotech |
10 mL |
EUR 261.6 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-20-100 |
Spherotech |
100 mL |
EUR 1668 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Carboxyl Magnetic Particles |
|
CM-200-10 |
Spherotech |
10 mL |
EUR 469.2 |
|
Description: Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
Nonetheless, EPS and acid manufacturing had been each diminished in L. casei CP002616 pLC2WΔ-HT (mutant pressure cured by high-temperature warmth remedy technique), demonstrating a distinction between these 2 curing strategies. Sequence evaluation of pLC2W and plasmid curing information recommend that plasmid pLC2W shouldn’t be concerned in EPS synthesis.
