The obligate intracellular bacterium Chlamydia psittaci is a recognized avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to important mortality if inadequately recognized and handled. Though intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been troublesome to show host-pathogen interactions in C. psittaci an infection as a result of lack of easy-to-handle genetic manipulation instruments. Right here, we present the genetic transformation of C. psittaci utilizing a plasmid shuttle vector that accommodates a controllable gene induction system.
The 7,553-bp plasmid p01DC12 was ready from the nonavian C. psittaci pressure 01DC12. We constructed the shuttle vector pCps-Tet-mCherry utilizing the complete sequence of p01DC12 and the 4,449-bp fragment of Chlamydia trachomatis shuttle vector pBOMB4-Tet-mCherry. pCps-Tet-mCherry contains genes encoding the inexperienced fluorescent protein (GFP), mCherry, and ampicillin resistance (AmpR). Goal genes may be inserted at a a number of cloning website (MCS). Importantly, these genes may be regulated by a tetracycline-inducible (tet) promoter.
Utilizing the pCps-Tet-mCherry plasmid shuttle vector, we present the expression of GFP, in addition to the induction of mCherry expression, in C. psittaci pressure 02DC15, which belongs to the avian C. psittaci 6BC clade. Moreover, we demonstrated that pCps-Tet-mCherry was stably retained in C. psittaci transformants. Thus, our C. psittaci plasmid shuttle vector system represents a novel focused strategy that permits the elucidation of host-pathogen interactions. The appliance of the pCps-Tet-mCherry shuttle vector system allows a promising new strategy to research unknown gene features of this pathogen.
Psittacosis, brought on by avian C. psittaci, has a serious financial influence within the poultry business worldwide and represents a big threat for zoonotic transmission to people. Prior to now decade, the instruments of genetic manipulation have been improved for chlamydial molecular research. Whereas a number of genetic instruments have been primarily developed in Chlamydia trachomatis, a steady gene-inducible shuttle vector system has to not date been out there for C. psittaci On this examine, we tailored a C. trachomatis plasmid shuttle vector system to C. psittaci We constructed a C. psittaci plasmid spine shuttle vector referred to as pCps-Tet-mCherry. The assemble expresses GFP in C. psittaci Importantly, exogeneous genes may be inserted at an MCS and are regulated by a tet promoter.
Detection of A number of Transgene Fragments in a Mouse Mannequin of Gene Doping Primarily based on Plasmid Vector Utilizing TaqMan-qPCR Assay
The World Anti-Doping Company has prohibited gene doping within the context of progress in gene remedy. There’s a threat that the augmentation of genes utilizing plasmids could possibly be utilized for gene doping. Nevertheless, no gold customary technique to detect this has been established. Right here, we aimed to develop a way to detect a number of transgene fragments as proof of gene doping. Firstly, gene supply mannequin mice as a mimic of gene doping had been created by injecting firefly luciferase plasmid with polyethylenimine (PEI) into the belly cavity. The outcomes confirmed profitable institution of the mannequin, with enough luminescence upon in vivo imaging.
Subsequent, a number of transgene fragments within the mannequin had been detected in plasma cell-free (cf)DNA, blood-cell-fraction DNA, and stool DNA utilizing the TaqMan- quantitative real-time PCR(qPCR) assay, with the best ranges in plasma cfDNA. Utilizing only a single drop of entire blood from the mannequin, we additionally tried long-term detection. The outcomes confirmed that a number of transgene fragments had been detected till 11 days. These findings point out that the mix of plasma cfDNA or only one drop of entire blood with TaqMan-qPCR assay is possible to detect plasmid-PEI-based gene doping. Our findings might speed up the event of strategies for detecting gene doping in people.
With the rise of recent highly effective genome engineering applied sciences, reminiscent of CRISPR/Cas9, cell fashions may be engineered successfully to speed up primary and illness analysis. Probably the most crucial step on this process is the environment friendly supply of overseas nucleic acids into cells by mobile transfection. Because the vectors encoding the elements essential for CRISPR/Cas genome engineering are all the time giant (9-19 kb), they end in low transfection effectivity and cell viability, and thus subsequent choice or purification of optimistic cells is required.
To beat these obstacles, we right here present a non-toxic and non-viral supply technique that will increase transfection effectivity (as much as 40-fold) and cell viability (as much as 6-fold) in plenty of hard-to-transfect human most cancers cell strains and first blood cells. At its core, the approach is predicated on including exogenous small plasmids of an outlined dimension to the transfection combination.
The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene supply vector
Non-viral gene supply methods have nice potential for protected and environment friendly gene remedy, whereas inefficient mobile and nuclear uptake stay as the key hurdles. Novel approaches are wanted to boost the transfection effectivity of non-viral vectors. In accordance with this want, the target of this examine was to assemble a non-viral vector that would obtain gene supply with out utilizing further lipid-based transfection agent. We aimed to impart self-delivery property to a non-viral vector by utilizing the cell and nucleus penetrating properties of YopM proteins from the three Yersinia spp. (Y. pestis, Y. enterocolotica and Y. pseudotuberculosis). Plasmid DNA (pDNA) encoding inexperienced fluorescent protein (GFP) was labeled with quantum dots (QDs) by way of peptide-nucleic acid (PNA) recognition website.
Recombinant YopM protein was then connected to the conjugate by way of a second PNA recognition website. The YopM ̶ QDs ̶ pDNA conjugate was transfected into HeLa cells with out utilizing further transfection reagent. All three conjugates produced GFP fluorescence, indicating that the plasmid was efficiently delivered to the nucleus. As management, bare pDNA was transfected into the cells by utilizing a business transfection reagent.
 Ready? PCR Mix |
|
M1127-1000 |
Biovision |
each |
EUR 614.4 |
Ready? PCR Mix |
|
M1127-200 |
Biovision |
each |
EUR 229.2 |
 Rigor? PCR Mix |
|
M1132-200 |
Biovision |
each |
EUR 385.2 |
 Breeze? PCR Mix |
|
M1134-200 |
Biovision |
each |
EUR 320.4 |
 Distant? PCR Mix |
|
M1136-200 |
Biovision |
each |
EUR 352.8 |
 Advance? PCR Mix |
|
M1139-200 |
Biovision |
each |
EUR 451.2 |
 Egg Drop Syndrome RT PCR kit |
|
RTq-V026-100D |
Bioingentech |
100T |
EUR 860.4 |
Egg Drop Syndrome RT PCR kit |
|
RTq-V026-150D |
Bioingentech |
150T |
EUR 969.6 |
Egg Drop Syndrome RT PCR kit |
|
RTq-V026-50D |
Bioingentech |
50T |
EUR 717.6 |
 lyophilised real-time PCR Master Mix for dual labeled probes |
|
M-PCR-156L |
MiTeGen |
960 reactions x 20 µl |
EUR 900.68 |
|
|
|
Description: lyophilised real-time PCR Master Mix for dual labeled probes |
lyophilised real-time PCR Master Mix for dual labeled probes |
|
M-PCR-156S |
MiTeGen |
192 reactions x 20 µl |
EUR 225.3 |
|
|
|
Description: lyophilised real-time PCR Master Mix for dual labeled probes |
 Individual Reaction Mix 1 |
|
G065-1 |
ABM |
200 reactions |
EUR 200.4 |
) dNTP Mix (1 ml) |
|
9002-1 |
Biovision |
each |
EUR 183.6 |
 Methylamp PCR Enhancer |
|
R-1002 |
EpiGentek |
|
- 400 Reactions
- 400 Reactions
|
 PCR-EZ D-PCR MASTER MIX |
|
BS294 |
Bio Basic |
100RXN, 100prep |
EUR 112.2 |
|
|
 amfiSure PCR Master Mix |
|
P0311-010 |
GenDepot |
2x50 rxns |
EUR 151.2 |
amfiSure PCR Master Mix |
|
P0311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure PCR Master Mix |
|
P0311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure PCR Master Mix |
|
P0311-100 |
GenDepot |
10x1ml |
EUR 343.2 |
amfiSure PCR Master Mix |
|
P0311-125 |
GenDepot |
15X50 rxns |
EUR 333.6 |
amfiSure PCR Master Mix |
|
P0311-200 |
GenDepot |
20x1ml |
EUR 562.8 |
amfiSure PCR Master Mix |
|
P0311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure PCR Master Mix |
|
P0311-500 |
GenDepot |
100x50 rxns |
EUR 1509.6 |
 amfiXpand PCR Master Mix |
|
P0331-010 |
GenDepot |
2X50 rxns |
EUR 181.2 |
amfiXpand PCR Master Mix |
|
P0331-025 |
GenDepot |
5X50 rxns |
EUR 327.6 |
amfiXpand PCR Master Mix |
|
P0331-050 |
GenDepot |
10X50 rxns |
EUR 537.6 |
amfiXpand PCR Master Mix |
|
P0331-250 |
GenDepot |
50x50 rxns |
EUR 2247.6 |
 Ready? PCR Mix-Dye |
|
M1128-1000 |
Biovision |
each |
EUR 614.4 |
Ready? PCR Mix-Dye |
|
M1128-200 |
Biovision |
each |
EUR 229.2 |
 Image Ready? PCR Mix |
|
M1129-200 |
Biovision |
each |
EUR 352.8 |
 Robust Ready? PCR Mix |
|
M1130-1000 |
Biovision |
each |
EUR 738 |
Robust Ready? PCR Mix |
|
M1130-200 |
Biovision |
each |
EUR 255.6 |
 Rigor? PCR Mix-Dye |
|
M1133-200 |
Biovision |
each |
EUR 385.2 |
 Breeze? PCR Mix-Dye |
|
M1135-200 |
Biovision |
each |
EUR 320.4 |
 Distant? PCR Mix-Dye |
|
M1137-200 |
Biovision |
each |
EUR 352.8 |
 Image Distant? PCR Mix |
|
M1138-200 |
Biovision |
each |
EUR 385.2 |
 Advance? PCR Mix-Dye |
|
M1140-200 |
Biovision |
each |
EUR 451.2 |
 Fire Start? PCR Mix |
|
M1141-200 |
Biovision |
each |
EUR 451.2 |
 Whole Blood PCR Mix |
|
M1143-200 |
Biovision |
each |
EUR 385.2 |
 Egg Drop Syndrome One-Step PCR kit |
|
Oneq-V026-100D |
Bioingentech |
100T |
EUR 1039.2 |
Egg Drop Syndrome One-Step PCR kit |
|
Oneq-V026-150D |
Bioingentech |
150T |
EUR 1177.2 |
Egg Drop Syndrome One-Step PCR kit |
|
Oneq-V026-50D |
Bioingentech |
50T |
EUR 861.6 |
 RT PCR kit) The egg drop syndrome (76) RT PCR kit |
|
RTq-V044-100D |
Bioingentech |
100T |
EUR 860.4 |
The egg drop syndrome (76) RT PCR kit |
|
RTq-V044-150D |
Bioingentech |
150T |
EUR 969.6 |
The egg drop syndrome (76) RT PCR kit |
|
RTq-V044-50D |
Bioingentech |
50T |
EUR 717.6 |
 lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 789.36 |
|
|
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 197.34 |
|
|
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
 PRICE DROP |
|
110-012 |
GeneOn |
4 x 1000 µl |
EUR 159.6 |
PRICE DROP |
|
110-012L |
GeneOn |
5x 4 x 1000 µl |
EUR 621.6 |
PRICE DROP |
|
110-012XL |
GeneOn |
10x 4 x 1000 µl |
EUR 1126.8 |
 MyTaq Blood PCR Mix, 2x |
|
BIO-25053/S |
Bioline |
Sample |
Ask for price |
MyTaq Blood PCR Mix, 2x |
|
BIO-25054 |
Bioline |
250 Reactions |
Ask for price |
 amfiSure Prime PCR Master Mix |
|
P1311-025 |
GenDepot |
5X50 rxns |
EUR 193.2 |
amfiSure Prime PCR Master Mix |
|
P1311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure Prime PCR Master Mix |
|
P1311-125 |
GenDepot |
15X50 rxns |
EUR 327.6 |
amfiSure Prime PCR Master Mix |
|
P1311-500 |
GenDepot |
100x50 rxns |
EUR 1485.6 |
 amfiSure Advanced PCR Master Mix |
|
P2311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure Advanced PCR Master Mix |
|
P2311-050 |
GenDepot |
10X50 rxns |
EUR 267.6 |
amfiSure Advanced PCR Master Mix |
|
P2311-125 |
GenDepot |
15X50 rxns |
EUR 338.4 |
amfiSure Advanced PCR Master Mix |
|
P2311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure Advanced PCR Master Mix |
|
P2311-500 |
GenDepot |
100x50 rxns |
EUR 1534.8 |
) mRNAExpress Tail PCR mix (5uM) |
|
MR-TAIL-PR |
SBI |
100 ul |
EUR 196.8 |
|
|
 Robust Ready? PCR Mix-Dye |
|
M1131-1000 |
Biovision |
each |
EUR 738 |
Robust Ready? PCR Mix-Dye |
|
M1131-200 |
Biovision |
each |
EUR 255.6 |
 Fire Start? PCR Mix-Dye |
|
M1142-200 |
Biovision |
each |
EUR 451.2 |
) AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE) |
|
MAG-PCR-NM-1 |
CORNING |
1/pk |
EUR 87.6 |
|
Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
 One-Step PCR kit) The egg drop syndrome (76) One-Step PCR kit |
|
Oneq-V044-100D |
Bioingentech |
100T |
EUR 1039.2 |
The egg drop syndrome (76) One-Step PCR kit |
|
Oneq-V044-150D |
Bioingentech |
150T |
EUR 1177.2 |
The egg drop syndrome (76) One-Step PCR kit |
|
Oneq-V044-50D |
Bioingentech |
50T |
EUR 861.6 |
) AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE) |
|
MAG-PCR-CL-1 |
CORNING |
1/pk |
EUR 69.6 |
|
Description: Bioscience Mag Beads; Magnetic PCR Clean Up |
) PCR Tubes (50) |
|
PCR-50 |
BioAssay Systems |
50 |
EUR 15 |
|
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes. |
 Bluetongue virus 1 PCR kit |
|
PCR-V226-48R |
Bioingentech |
50T |
EUR 987.6 |
Bluetongue virus 1 PCR kit |
|
PCR-V226-96R |
Bioingentech |
100T |
EUR 1335.6 |
 PCR kit) Poliovirus 1 (PV1) PCR kit |
|
PCR-H659-48R |
Bioingentech |
50T |
EUR 987.6 |
Poliovirus 1 (PV1) PCR kit |
|
PCR-H659-96R |
Bioingentech |
100T |
EUR 1335.6 |
 Psittacid herpesvirus 1 PCR kit |
|
PCR-V014-48D |
Bioingentech |
50T |
EUR 543.6 |
Psittacid herpesvirus 1 PCR kit |
|
PCR-V014-96D |
Bioingentech |
100T |
EUR 686.4 |
 Equine herpesvirus 1 PCR kit |
|
PCR-V144-48D |
Bioingentech |
50T |
EUR 543.6 |
Equine herpesvirus 1 PCR kit |
|
PCR-V144-96D |
Bioingentech |
100T |
EUR 686.4 |
 Bovine herpesvirus 1 PCR kit |
|
PCR-V182-48D |
Bioingentech |
50T |
EUR 543.6 |
Bovine herpesvirus 1 PCR kit |
|
PCR-V182-96D |
Bioingentech |
100T |
EUR 686.4 |
 Taq 5x PCR Mix 12.5 mM |
|
BT10402 |
Bioatlas |
250rxn |
EUR 130.8 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
 Taq 2x PCR Mix 100 rxn |
|
BA01501 |
Bioatlas |
100rxn |
EUR 112.8 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
 HotTaq 2x PCR Mix 100 rxn |
|
BA01503 |
Bioatlas |
100rxn |
EUR 144 |
|
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
 RedTaq 2x PCR Mix 100 rxn |
|
BA01507 |
Bioatlas |
100rxn |
EUR 175.2 |
|
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
) amfiSure ONE PCR Master Mix(2X) |
|
P7000-005 |
GenDepot |
5x1 ml |
EUR 262.8 |
amfiSure ONE PCR Master Mix(2X) |
|
P7000-010 |
GenDepot |
10x1 ml |
EUR 380.4 |
amfiSure ONE PCR Master Mix(2X) |
|
P7000-050 |
GenDepot |
50x1 ml |
EUR 1453.2 |
amfiSure ONE PCR Master Mix(2X) |
|
P7000-100 |
GenDepot |
100x1 ml |
EUR 2522.4 |
 qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-360L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
|
|
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-360S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
|
|
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
 qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-362L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
|
|
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-362S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
|
|
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service |
 TuMinute PCR Clean-Up Kit |
|
P-1005 |
EpiGentek |
-
EUR 303.60
-
EUR 90.20
-
EUR 171.60
|
- 100 Samples
- 50 Samples
- 100 Samples
|
 qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-361L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
|
|
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-361S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
|
|
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
 qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-363L |
MiTeGen |
10 x 1,25 ml |
EUR 864.5 |
|
|
|
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-363S |
MiTeGen |
2 x 1,25 ml |
EUR 216.19 |
|
|
|
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
 qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|
M-PCR-321L |
MiTeGen |
10 x 1,25 ml |
EUR 1438.81 |
|
|
|
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|
M-PCR-321S |
MiTeGen |
2 x 1,25 ml |
EUR 359.77 |
|
|
|
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
 MULTIPLEX KIT PCR MASTITIS PCR kit |
|
PCR-MPX218-48D |
Bioingentech |
50T |
EUR 543.6 |
MULTIPLEX KIT PCR MASTITIS PCR kit |
|
PCR-MPX218-96D |
Bioingentech |
100T |
EUR 686.4 |
 Caprine herpes virus 1 PCR kit |
|
PCR-V312-48D |
Bioingentech |
50T |
EUR 543.6 |
Caprine herpes virus 1 PCR kit |
|
PCR-V312-96D |
Bioingentech |
100T |
EUR 686.4 |
 Mouse parvovirus type 1 PCR kit |
|
PCR-V324-48D |
Bioingentech |
50T |
EUR 543.6 |
Mouse parvovirus type 1 PCR kit |
|
PCR-V324-96D |
Bioingentech |
100T |
EUR 686.4 |
 Borna disease viruses 1 PCR kit |
|
PCR-V605-48R |
Bioingentech |
50T |
EUR 987.6 |
Borna disease viruses 1 PCR kit |
|
PCR-V605-96R |
Bioingentech |
100T |
EUR 1335.6 |
 Herpes simplex type 1 PCR kit |
|
PCR-H426-48D |
Bioingentech |
50T |
EUR 543.6 |
Herpes simplex type 1 PCR kit |
|
PCR-H426-96D |
Bioingentech |
100T |
EUR 686.4 |
 Dengue virus type 1 PCR kit |
|
PCR-H521-48R |
Bioingentech |
50T |
EUR 987.6 |
Dengue virus type 1 PCR kit |
|
PCR-H521-96R |
Bioingentech |
100T |
EUR 1335.6 |
 Canine adenovirus type 1 PCR kit |
|
PCR-V077-48D |
Bioingentech |
50T |
EUR 543.6 |
Canine adenovirus type 1 PCR kit |
|
PCR-V077-96D |
Bioingentech |
100T |
EUR 686.4 |
 qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-364L |
MiTeGen |
10 x 1,25 ml |
EUR 864.5 |
|
|
|
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-364S |
MiTeGen |
2 x 1,25 ml |
EUR 216.19 |
|
|
|
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
 qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-365L |
MiTeGen |
10 x 1,25 ml |
EUR 864.5 |
|
|
|
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
|
M-PCR-365S |
MiTeGen |
2 x 1,25 ml |
EUR 216.19 |
|
|
|
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service |
 qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|
M-PCR-322L |
MiTeGen |
10 x 1,25 ml |
EUR 1438.81 |
|
|
|
Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
|
M-PCR-322S |
MiTeGen |
2 x 1,25 ml |
EUR 359.77 |
|
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Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
 qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
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M-PCR-323L |
MiTeGen |
10 x 1,25 ml |
EUR 1438.81 |
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Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
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M-PCR-323S |
MiTeGen |
2 x 1,25 ml |
EUR 359.77 |
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Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service |
 MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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PCR-MPX401-48D |
Bioingentech |
50T |
EUR 543.6 |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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PCR-MPX401-96D |
Bioingentech |
100T |
EUR 686.4 |
) HotStart PCR Master mix (2X, Green Dye) |
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BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
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HotStart PCR Master mix (2X, Green Dye) |
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BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
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) Taq PCR Master Mix (2X, Blue Dye) |
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BS9295 |
Bio Basic |
1ml |
EUR 101.76 |
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The Y. pseudotuberculosis YopM-functionalized conjugate achieved the best GFP expression, in comparison with different two YopM proteins and the transfection reagent. To the very best of our data, YopM protein was used for the primary time in a non-viral gene supply vector.
