Anti–BSA antibodies are a significant explanation for non-specific binding in insulin autoantibody radiobinding assays.
The affect of antibody purposeful affinity on the effector features concerned within the clearance of circulating immune complexes anti–BSA IgG/BSA.
Anti–BSA antibodies don’t cross-react with the 69-kDa islet cell autoantigen ICA69.
In distinction to a number of beforehand printed stories, we show by a wide range of antibody assays that bovine serum albumin (BSA) isn’t antigenically cross-reactive with the 69-kDa islet cell autoantigen (ICA69). Quick protein liquid chromatography purified BSA and extremely purified recombinant human ICA69 had been used to determine delicate Western blot and ELISA assays as a way to detect antibodies towards these two proteins.
The assays excluded BSA or powdered milk as blocking brokers, since these would intervene with antibody binding. A panel of sera from diabetic people, first diploma relations, and regular controls confirmed that almost all of people from every group had antibodies towards ICA69 as assayed by Western blots, whereas significantly fewer had anti-BSA antibodies on Western blots, and people with antibodies to each proteins occurred solely not often.
To discover this difficulty additional we immunized a complete of 16 particular person rats, representing 4 completely different strains (bio-breeding diabetes resistant and diabetes susceptible, Wistar-Furth, and Sprague-Dawley), with both BSA (n = 2 of every pressure) or with recombinant ICA69, and for every animal pre- and postimmune antibody titres towards BSA and towards ICA69 had been assayed individually by enzyme-linked immunoabsorbent assay.
In rats immunized with BSA, anti-BSA titres elevated about 100,000-fold over preimmune ranges, whereas anti-ICA69 reactive antibodies had been unchanged over preimmune ranges. Equally, in rats immunized with ICA69, anti-ICA69 titres rose about 100,000-fold over preimmune ranges, whereas anti-BSA antibodies had been unchanged over preimmune ranges. Thus we discover no proof for the existence of antibody cross-reactivity between ICA69 and BSA, both in people or in immunized rats.
Rat Anti acrosin antibodies ELISA kit |
|||
E02A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti acrosin antibodies ELISA kit |
|||
E02A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti acrosin antibodies ELISA kit |
|||
E02A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti acrosin antibodies ELISA kit |
|||
E08A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti acrosin antibodies ELISA kit |
|||
E08A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti acrosin antibodies ELISA kit |
|||
E08A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti acrosin antibodies ELISA kit |
|||
E07A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti acrosin antibodies ELISA kit |
|||
E07A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti acrosin antibodies ELISA kit |
|||
E07A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti acrosin antibodies ELISA kit |
|||
E06A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti acrosin antibodies ELISA kit |
|||
E06A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti acrosin antibodies ELISA kit |
|||
E06A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti acrosin antibodies ELISA kit |
|||
E03A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti acrosin antibodies ELISA kit |
|||
E03A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti acrosin antibodies ELISA kit |
|||
E03A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Anti acrosin antibodies ELISA kit |
|||
E01A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Human Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Anti acrosin antibodies ELISA kit |
|||
E01A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Human Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Anti acrosin antibodies ELISA kit |
|||
E01A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Human Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti acrosin antibodies ELISA kit |
|||
E09A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Monkey Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti acrosin antibodies ELISA kit |
|||
E09A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Monkey Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti acrosin antibodies ELISA kit |
|||
E09A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Monkey Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
|||
E04A0718-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
|||
E04A0718-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti acrosin antibodies ELISA kit |
|||
E04A0718-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti acrosin antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti Apolipoprotein Antibodies ELISA kit |
|||
E02A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti Apolipoprotein Antibodies ELISA kit |
|||
E02A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti Apolipoprotein Antibodies ELISA kit |
|||
E02A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti Apolipoprotein Antibodies ELISA kit |
|||
E07A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti Apolipoprotein Antibodies ELISA kit |
|||
E07A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti Apolipoprotein Antibodies ELISA kit |
|||
E07A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Porcine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti Apolipoprotein Antibodies ELISA kit |
|||
E08A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti Apolipoprotein Antibodies ELISA kit |
|||
E08A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti Apolipoprotein Antibodies ELISA kit |
|||
E08A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Canine Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse anti- prothrombins antibodies ELISA Kit |
|||
ELA-E0319m | Lifescience Market | 96 Tests | EUR 1038 |
Goat Anti Apolipoprotein Antibodies ELISA kit |
|||
E06A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti Apolipoprotein Antibodies ELISA kit |
|||
E06A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti Apolipoprotein Antibodies ELISA kit |
|||
E06A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Goat Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti Apolipoprotein Antibodies ELISA kit |
|||
E03A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti Apolipoprotein Antibodies ELISA kit |
|||
E03A0447-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti Apolipoprotein Antibodies ELISA kit |
|||
E03A0447-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Anti Apolipoprotein Antibodies ELISA kit |
|||
E01A0447-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Human Anti Apolipoprotein Antibodies in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
When our rat anti-BSA antisera had been used to probe Western blots made out of rat islets remoted within the strict absence of fetal calf serum, we had been unable to detect a 69-kDa band, even when the islets had been preincubated with gamma-interferon, a therapy which has been reported to induce the BSA cross-reactive islet antigen. We conclude that BSA isn’t antigenically cross-reactive with ICA69 on the antibody stage, and it’s extremely unlikely that BSA is antigenically cross-reactive with another 69 kD islet cell antigen.