Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium, generally employed to deal with sure gastrointestinal issues. It’s quick rising as an vital goal for the event of therapeutic engineered micro
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Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaciDevelopment of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci
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The obligate intracellular bacterium Chlamydia psittaci is a recognized avian pathogen inflicting psittacosis in birds and is able to zoonotic transmission. In human pulmonary infections, C. psittaci could cause pneumonia related to important mortality
![[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]](https://molvisindex.org/kg/uploads/2021/08/IMG-20210817-WA0017.jpg)
[Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene][Maintenance of Plasmid Expression in vivo Depends Primarily on the CpG Contents of the Vector and Transgene]
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Plasmid-mediated gene remedy, being a protected and comparatively cheap therapeutic technique, is stricken by a quick silencing of transgene expression. The silencing severely reduces the long-term effectivity of plasmid vectors.
Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida.
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Genome engineering of non-conventional microorganisms requires the event of devoted artificial biology instruments. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium broadly used for metabolic engineering owing to its versatile metabolism and
Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.
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Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the operate of a repABC operon and have been categorised into 4 incompatibility teams, particularly, incRh1, incRh2, incRh3,